We narrowed to 16,985 results for: She
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PD-1-miSFIT-4x
Plasmid#124678PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV_Cdx2 HMEJ donor
Plasmid#97319PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.DepositorInsertCdx2 HMEJ donor
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A-NG-nCas9
Plasmid#163536PurposeMammalian CG-to-GC base editingDepositorInserteA3A-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD1-EGFP
Plasmid#131206Purposemammalian expression, tet RDepositorInsertNOD1 (NOD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 XBB Spike
Plasmid#195287PurposeExpresses codon-optimized full length SARS-CoV-2 XBB SpikeDepositorInsertSARS-CoV-2 XBB Spike (S )
UseTagsExpressionMammalianMutationContains the following mutations: T19I, LPPA24-27…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceFeb. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJET-CMV-GFP-P2A-K20-P2A-mCherry
Plasmid#185618PurposeRibosomal stalling reporter: positive controlDepositorInsertGFP-K20(AAA)-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-K0-P2A-mCherry
Plasmid#185619PurposeRibosomal stalling reporter: emptyDepositorInsertGFP-empty-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-I15-P2A-mCherry
Plasmid#185620PurposeRibosomal stalling reporter: 15x isoleucineDepositorInsertGFP-I15-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_051_dCas9-KRAB-HA
Plasmid#228936PurposeAll-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNADepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-C1483F
Plasmid#69008Purposeactivating MTOR mutationDepositorInsertMTOR-C1483F (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Cysteine 1483 to PhenylalaninePromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE248 pCDNA3.1+zeo-VSV-KLHL12
Plasmid#16761DepositorInsertpCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)
UseTagsVSVExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP
Plasmid#163030PurposeExpression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianMutationPromoterAlb-AATAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-GFP-BRCA1
Plasmid#71116Purposemammalian expression of GFP-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWZ699
Plasmid#208117PurposePayload yeast assembly vector with TK-mActb on backboneDepositorTypeEmpty backboneUseSynthetic Biology; Copy number inducibleTagsExpressionBacterial, Mammalian, and Y…MutationPromoterHuman PGK1 for thymidine kinaseAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato
Plasmid#171027PurposeExpresses ChrimsonR-tdTomato fusion after Flp-dependent recombinationDepositorHas ServiceAAV1 and AAV9InsertChrimsonR-tdTomato
UseAAVTagsChrimsonR is fused to tdTomatoExpressionMutationPromoterEf1aAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1
Plasmid#71858Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD1
Plasmid#195839PurposeExpresses human FZD1 with an N-terminal HiBiTDepositorInsertFZD1 (FZD1 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV4Neo-murine IL-17RA-HA
Plasmid#46859Purposeexpresses mouse IL-17RADepositorInsertIL-17RA (Il17ra Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)
Plasmid#229828PurposeA Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBABE-HA-GOLPH3
Plasmid#21687DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_Luciferase_sgRNA
Plasmid#74190Purposelentiviral vector expressing sgRNA targeting LuciferaseDepositorInsertLuciferase sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-WPRE-bGH-DEST (JDW 1309)
Plasmid#229815PurposeA CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcriptsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF2
Plasmid#200385PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Anc689-NG-nCas9
Plasmid#163527PurposeMammalian CG-to-GC base editingDepositorInsertAnc689-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GRK3
Plasmid#32689DepositorInsertGRK3 (GRK3 Bovine)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-XL-AncBE4max
Plasmid#136254PurposeDoxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4maxDepositorInsertAncBE4max_GFP
UseCRISPR; Piggybac dna transposonTagsExpressionMammalianMutationThe AncBE4max_GFP insert was PCR amplified from a…PromoterTRE3GAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-dr-myl7-pro (JDW 1148)
Plasmid#229836PurposeA gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.DepositorInsertcmlc2/myl7
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)
Plasmid#214880PurposeLentiviral vector encoding RfxCas13d targeting CD46 guideDepositorInserthU6-crCD46-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_APOBEC1-UdgX-NG-nCas9
Plasmid#163533PurposeMammalian CG-to-GC base editingDepositorInsertAPOBEC1-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-nsp7-nsp8
Plasmid#159092PurposeCoexpression construct of Nsp7 with an N-terminal His-tag and Nsp8DepositorInsertsNSP7
NSP8
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF1
Plasmid#200384PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ403
Plasmid#208113PurposemSwAP-In marker cassette 1 with mScarlet-PuroTKDepositorInsertmScarlet-PuroTK
UseTagsP2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6f
Plasmid#128315PurposeExpresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorHas ServiceAAV1InsertGCaMP6f
UseAAVTagsHis TagExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9-UdgX
Plasmid#163566PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9-UdgX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD7
Plasmid#195845PurposeExpresses human FZD7 with an N-terminal HiBiTDepositorInsertFZD7 (FZD7 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE)
Plasmid#214883PurposeLentiviral vector encoding RfxCas13d targeting PURI guide arrayDepositorInserthU6-crPURI-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE)
Plasmid#214884PurposeLentiviral vector encoding RfxCas13d targeting GLY guide arrayDepositorInserthU6-crGLY-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5 bovine GRK5
Plasmid#14690DepositorInsertGRK5 (GRK5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-BRCA1
Plasmid#71117Purposemammalian expression of Flag-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV/TOAvailable sinceNov. 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-FLAG-MTOR-I2500F
Plasmid#69014Purposeactivating MTOR mutationDepositorInsertMTOR-I2500F (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Isoleucine 2500 to PhenylalaninePromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTargetF-FRT
Plasmid#113637PurposeDerivative of pTargetF with gRNA targeting FRT siteDepositorInsertsgRNA-FRT
UseCRISPRTagsExpressionMutationPromoterpJ23119Available sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eArch 3.0-EYFP
Plasmid#35516PurposeAAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.DepositorInsertArch3.0-EYFP
UseAAVTagsEYFPExpressionMammalianMutationEnhanced trafficking signal and ER export signal …PromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#113398Purposeexpresses gRNA for Cas9 FRT targettingDepositorInsertexo, beta, gam, sgRNA-FRT
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-3xNLS-mTagBFP2-Cre (JDW 918)
Plasmid#224517PurposeA Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusionDepositorInsert3xNLS-mTagBFP2-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATF6-(S1P-)
Plasmid#32956PurposeFor visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.DepositorInsertATF6 (ATF6 Human)
UseTagsEGFPExpressionMammalianMutationS1Protease site mutation: R415A/R416APromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ATF6 (1-373)m1
Plasmid#27174PurposeFor mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.DepositorInsertATF6 (ATF6 Human)
UseTagsHAExpressionMammalianMutationContains only aa 1-373 Amino acids 315–317 are c…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only
