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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 40,651 results for: TRO

Showing: 10751 - 10800 of 40651 results

  1. pGAN147

    Plasmid
    #129202
    Purpose
    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone
    Depositor
    Ahmad Khalil
    Article
    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    129202-plasmid-map-sequence-id-255858

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  15. TR-Csy4-H29A

    Plasmid
    #80602
    Purpose
    Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    Csy4-H29A
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    His29 mutated to Ala
    Promoter
    CBA
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    80602-plasmid-map-sequence-id-152080

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  41. pTJV1Sc2-tetA

    Plasmid
    #166982
    Purpose
    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    CspRecT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166982-plasmid-map-sequence-id-331335

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  43. plentiCRISPR-sgGOT1

    Plasmid
    #72874
    Purpose
    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    sgGOT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only

  45. pCS2+/N-SNAPf

    Plasmid
    #184415
    Purpose
    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184415-plasmid-map-sequence-id-365684

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  46. pCS2+/C-SNAPf

    Plasmid
    #184416
    Purpose
    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184416-plasmid-map-sequence-id-362158

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  47. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid
    #200408
    Purpose
    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    iRFP720 (frameshift mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200408-plasmid-map-sequence-id-396809

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  49. pRDA_186

    Plasmid
    #133458
    Purpose
    U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133458-plasmid-map-sequence-id-261410

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Showing: 10751 - 10800 of 40651 results