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159,258 results

Showing: 8801 - 8840 of 159258 results

  4. CCR10-DuET

    Plasmid
    #213197
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR10 (CCR10 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213197-plasmid-map-sequence-id-430761

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  5. pHB-3

    Plasmid
    #140956
    Purpose
    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.
    Depositor
    Kang Zhou
    Article
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    140956-plasmid-map-sequence-id-334291

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  8. ZsGreen1-N1

    Plasmid
    #54702
    Purpose
    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ZsGreen1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54702-plasmid-map-sequence-id-266653

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  12. PB-PEmax

    Plasmid
    #187893
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187893-plasmid-map-sequence-id-370734

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  14. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

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  15. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91130-plasmid-map-sequence-id-182254

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  16. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  18. mEmerald-Lifeact-7

    Plasmid
    #54148
    Purpose
    Localization: Actin, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. GRM5-DuET

    Plasmid
    #213303
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM5 (GRM5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213303-plasmid-map-sequence-id-431813

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  21. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid
    #224356
    Purpose
    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)
    Depositor
    Nicolas Charlet-Berguerand
    Article
    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.
    Insert
    uN2C with a GGC repeat expansion (100x)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Codon-optimized for expression in human, so no pu…
    Promoter
    CMV + chimeric intron (CAG)
    Available since
    Feb. 5, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224356-plasmid-map-sequence-id-460492

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  22. pLEX-MCS PGK promoter

    Plasmid
    #128044
    Purpose
    Lentiviral vector with PGK promoter
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128044-plasmid-map-sequence-id-311647

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  27. psPAX2-D64V-NC-MS2

    Plasmid
    #122944
    Purpose
    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
    Depositor
    Baisong Lu
    Article
    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531
    Insert
    NC-MCP (MS2 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D64V
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122944-plasmid-map-sequence-id-239322

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  36. miReporter-PGK

    Plasmid
    #82477
    Purpose
    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi
    Insert
    H2B-mCherry and H2B-Citrine. HygroR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82477-plasmid-map-sequence-id-163461

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  38. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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Showing: 8801 - 8840 of 159258 results