We narrowed to 13,971 results for: EGFP
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GFP-Miro1 S156E
Plasmid#127589PurposePhospho-mimetic of S156DepositorAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-synaptoPAC-minWPRE
Plasmid#153085PurposeExpresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neuronsDepositorInsertsUseAAVTagsHis tag, mScarlet, and myc tagExpressionMammalianAvailable SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo_TAF3_Phd
Plasmid#179400PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_PhdDepositorInsertCBX7_Chromo - TAF3_Phd
TagsAviTag and EGFPExpressionMammalianPromoterCAGGSAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorInsertKif5c (Kif5c Rat)
TagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGMKOSiE
Plasmid#20865DepositorInsertmyelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
ExpressionMammalianAvailable SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MPRA-MluI-SpeI-EcoRI
Plasmid#190196PurposeEmpty vector for inserting MPRA libraries into AAVDepositorTypeEmpty backboneUseAAV; Massively parallel reporter assay (mpra)ExpressionMammalianAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-SBP-VAP-A*
Plasmid#120175PurposeExpresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-ADepositorInsertVAMP associated protein A (VAPA Human)
TagsEGFP and SBPExpressionMammalianMutationTruncated VAP-A: 118-249PromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Intersectin Short
Plasmid#47394DepositorAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC1GFP-Kir7.1
Plasmid#79118PurposeExpress N-terminal fused Kir7.1 protein in mammalian cellsDepositorInsertInwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-T2A-GFP
Plasmid#193781PurposeTricistronic expression of dCas9-EGFP-BSDDepositorInsertdead Cas9
UseLentiviralTagsFLAGExpressionMammalianPromoterIn frame with existing Cas9 promoterAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
hHS1-M7A,H102A
Plasmid#159171PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1-M7A,H102A
ExpressionMammalianMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterCMVAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMS-dsRed2-WT LMNA-GFP minigene
Plasmid#128230PurposeLamin A splicing reporterDepositorInsertlamin A (LMNA Human)
TagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP L273D-L276D
Plasmid#114182PurposeORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
c1GFP-W53XKir7.1
Plasmid#107183PurposeGenerates Nonsense mutant cloneDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M
Plasmid#114180PurposeORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS-shCHD5 #1
Plasmid#68876PurposeCHD5 shRNA expressed from Adeno-associated viral (AAV) vectorDepositorInsertCHD5
UseAAV and RNAiExpressionMammalianPromoterH1Available SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-nArgBP2ΔNSE
Plasmid#74515PurposeExpress GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.DepositorAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LpIA(wild-type)
Plasmid#61821PurposeEncodes a wild-type sequence of LplA and serves as the negative control for resorufin labelingDepositorInsertE. coli lipoic acid ligase
Tags6XHis, EGFP, and FlagExpressionMammalianPromoterCMVAvailable SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-WT (AF41)
Plasmid#21367DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
TagsEGFP and FLAGExpressionMammalianMutationwild-type sequence*Available SinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS-shCHD5 #2
Plasmid#68877PurposeCHD5 shRNA expressed from Adeno-associated viral (AAV) vectorDepositorInsertCHD5
UseAAV and RNAiExpressionMammalianPromoterH1Available SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-BLM DM
Plasmid#80071PurposeExpression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutationsDepositorInsertBLM (BLM Human)
TagsGFP-BLMExpressionMammalianMutationK317R (AAA>AGA) and K331R (AAA>AGA)Available SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)
Plasmid#174630PurposePoorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)DepositorInsertKIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
TagsGFP-SSPB(micro)ExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…PromoterChicken beta-actinAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-IP3KB
Plasmid#32528DepositorAvailable SinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
NgΔIQ FUNgDIQGW
Plasmid#92232PurposeLentiviral expression of human Ng with deletion mutationDepositorInsertNg (NRGN Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdelta IQPromoterhUbCAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSin-GFP-Fg
Plasmid#174307PurposeLentiviral expression of GFPDepositorAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-alpha-E-Catenin 3xFP4 (895)
Plasmid#67936PurposeMammalian expression of alpha E cateninDepositorInsertalpha E catenin (Ctnna1 Mouse)
Tags3xFP4 and EGFPExpressionMammalianMutationN-terminal domainPromoterCMVAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-121
Plasmid#131126PurposeA-to-G base editing reporterDepositorInsertmutant EGFP
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV promoterAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Cx43-GFP
Plasmid#176860PurposeExpresses Cx43 fused with GFP at the C-terminus in astrocytesDepositorAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-Anillin shRNA
Plasmid#187270PurposeLentiviral expression of shRNA targeting AnillinDepositorAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
SOX17-NLS-tdTomato-EPG
Plasmid#210466PurposeDonor plasmid for knock-in NLS-tdTomato into the human SOX17 locusDepositorInsertSOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
UseCRISPRAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-synaptoPAC-minWPRE
Plasmid#153100PurposeCre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neuronsDepositorInsertsUseAAVTagsHis tag, mScarlet, and myc tagExpressionMammalianAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102888PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP
Plasmid#126698Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertsgRNA targeting human SCGB3A2 locus
ExpressionMammalianPromoterHuman U6Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
P119 C-Check
Plasmid#66817PurposeDual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cellsDepositorInserttruncated Enhanced Green Fluorescent Protein and asRED
ExpressionMammalianPromoterPGK and CMVAvailable SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-112
Plasmid#131127PurposeC-to-T base editing reporterDepositorInsertMutant EGFP
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV promoterAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)
Plasmid#174631PurposeOpto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)DepositorInsertKIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
TagsGFP-SSPB(micro)ExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…PromoterChicken beta-actinAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-GFP-PKHD1
Plasmid#108408PurposeCan be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.DepositorAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29(+exo) (LM2759)
Plasmid#208964PurposeUnfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS1424
Plasmid#29220PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertPle155-EGFP-NLS
UseHigh copy number homologous recombination plasmid…ExpressionMammalianPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable SinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemExpressionMammalianMutationY64L (mutRFP)Available SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMS-dsRed2-C1824U LMNA-GFP minigene
Plasmid#128231PurposeC1824U mutation Lamin A splicing reporterDepositorInsertlamin A (LMNA Human)
TagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianMutationC1824UPromoterCMVAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB112
Plasmid#180173PurposepAAV construct GluN1WT (shRNA resistant)DepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(G137I)
Plasmid#133274PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
TagsHAExpressionMammalianMutationG137IPromoterCMVd2Available SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS423-mitoT
Plasmid#174525PurposeExpresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranesDepositorInsertYeast mitochondrial intermembrane tether
UseSynthetic BiologyTagseGFPExpressionYeastPromoterMET25Available SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mutant Human Cortactin-GFP
Plasmid#71365Purposemammalian expression of mutant human Cortactin-GFPDepositorInsertcortactin (CTTN Human)
TagsEGFPExpressionMammalianMutationsilent mutation present at 72nd nucleotidePromoterCMVAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
KCC2-pHext
Plasmid#104076PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
TagspHluorine tag inserted in the second putative tra…ExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-GFP-AnillinFL
Plasmid#187272PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full lengthDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsEGFPPromoterU6, CMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only
