161,570 results
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KHC068 BRD2-KI-GFP-2A-L387A
Plasmid#231713PurposeCRISPR mediated KI of BromoTag at N-terminus of BRD2-GFP-P2A-BromoTag, works well with KHC064 and KHC065DepositorInsertBrd2 (BRD2 Human)
UseCRISPRTagsBromoTag and GFPExpressionMammalianMutationL387APromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_HA-tNGFR_PuroR
Plasmid#212652PurposepiggyBac vector to express HA-tNGFR with puromycin resistanceDepositorInserttNGFR
UsePiggybacTagsHA tagExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-CAGGS (JDW 912)
Plasmid#224472PurposeGateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.DepositorInsertCAGGS Promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)
Plasmid#224490PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-iRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a (JDW 1164)
Plasmid#224473PurposeGateway compatible 5' entry clone containing the human EF1a promoterDepositorInsertEF1a promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-mito
Plasmid#214415PurposeMitochondria localization of low-affinity NFAST fused to mApple-mitoDepositorInsertRspA-N
UseTagsmApple-mitoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-6xHp
Plasmid#214416PurposeLipid droplet localization of CFAST fused to Halotag-6xHpDepositorInsertdeletion
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BILF1
Plasmid#195797PurposeGateway Entry Clone of Epstein-Barr virus BILF1DepositorInsertBILF1 (BILF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BILF2
Plasmid#195788PurposeGateway Entry Clone of Epstein-Barr virus BILF2DepositorInsertBILF2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_rHCaMP
Plasmid#187107PurposeExpression of rHCaMP in mammalian cellsDepositorInsertrHCaMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P CISD1_2
Plasmid#160774PurposeSuppress CISD1DepositorInsertshCISD1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046159
Plasmid#145509PurposeYeast Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-hphMX6-3nmt1
Plasmid#105162PurposeYeast gene targetingDepositorInserthphMX6-3nmt1
UseTags3nmt1ExpressionBacterial and YeastMutationPromoter3nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag FKHR
Plasmid#13507DepositorInsertFKHR (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21
Plasmid#105522PurposeT7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Lrrc59
Plasmid#226564PurposeEncodes Lrrc59 protein labeled with mNeonGreenDepositorInsertLrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-ER-5
Plasmid#170570PurposeExpresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.DepositorInsertAPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
Plasmid#84239PurposeExpresses ABA-inducible VPR-Sp dCas9DepositorInsertsABI-tagBFP-Sp dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC SNAP-DDX6
Plasmid#119948PurposeLentiviral vector expressing SNAP-DDX6 fusion proteinDepositorInsertDDX6 (DDX6 Human)
UseLentiviralTagsSNAPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDESTsplice
Plasmid#32484DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IQGAP1
Plasmid#30112DepositorInsertIQGAP1 (IQGAP1 Human)
UseTagstandem EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA
Plasmid#197044PurposeIn situ cell-surface proteome extraction by extracellular labeling (iPEEL)DepositorInsertspCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA
UseTagsHA, mycExpressionMammalianMutationPromoterpCAGAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAtC
Plasmid#78098PurposePlant expression of Cas9 and gRNA empty backbone; Hygro ResistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT413
Plasmid#8606DepositorInsertbarnase, barstar
UseTagsphoA signal sequence to barnaseExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Neo GFP-Progerin
Plasmid#118710PurposeLentiviral TET-ON inducible GFP-ProgerinDepositorInsertGFP-Progerin (LMNA Human)
UseLentiviral; Tet-on inducibleTagsGFPExpressionMammalianMutationPromoterCMV TRE3G (TET-ON)Available sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0000 (No LUC)
Plasmid#44465DepositorTypeEmpty backboneUsePlant transformationTagsExpressionMutationPromoterAvailable sinceMay 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-hCas3
Plasmid#134920PurposeComponents for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.DepositorInsertCas3 with bpNLS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFr.WPRE.SV40
Plasmid#98929PurposeAAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-humanTRAF6 (1093)
Plasmid#66929PurposeMammalian Expression of TRAF6DepositorInsertTRAF6 (TRAF6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTBP
Plasmid#171073PurposeExpresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBPDepositorInsertTATA-box-binding protein (TBP Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMF-TetTight
Plasmid#193288PurposeLentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgeneDepositorInsertrtTA-P2A-tTS
UseLentiviralTagsExpressionMammalianMutationPromoterEF‐1αAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-p50
Plasmid#107977PurposeExpresssion of NF-kB p50DepositorInsertNF-kB p50 (Nfkb1 Mouse)
UseTagsN/AExpressionMammalianMutationAmino acids VQACI added to C-terminusPromoterCMVAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII_TOPO_hNEAT1
Plasmid#61518Purposehuman NEAT1_v1 short isoform 3.7kb insert in general cloning vectorDepositorInsertNEAT1 (NEAT1 Human)
UseGeneral cloning vectorTagsExpressionMutation*see comment belowPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SETDB1_WT
Plasmid#82291PurposeGateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.DepositorInsertSETDB1 (SETDB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 wt
Plasmid#16434DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only
