164,127 results

1. pL-CRISPR.EFS.GFP

   Plasmid

   #57818

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EFS and hU6

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

   Plasmid

   #234883

   Purpose

   non-targeting CRISPRi control

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2631

   Plasmid

   #144140

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   INSM1 (INSM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPS2037

   Plasmid

   #1370

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   PGK1-U1A-PGK1 3' UTR (PGK1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pLEX-TCRb-FKBP-Blasticidin

   Plasmid

   #120723

   Purpose

   Expresses ER-targeted recruiter protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   T cell receptor β chain
   

   Use

   Lentiviral

   Tags

   FKBP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MEF2C-HA

   Plasmid

   #32515

   Depositor

   Andrew Lassar

   Article

   Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

   Insert

   Mef2c (Mef2c Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGLO-GFP-1UAG

   Plasmid

   #82500

   Purpose

   Expresses GFP with 1 UAG codon at the amino acid position 3

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Green Fluorescent Protein with 1 UAG codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Added an UAG codon at the amino acid position 3

   Promoter

   AraBAD

   Available Since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1-SypHer3s-dmito

   Plasmid

   #108119

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-dmito
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. APEX2-V5-G3BP1_pTRE-G418

    Plasmid

    #202013

    Purpose

    expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    APEX2-V5-G3BP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-Tight

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. His6-MBP-uTEV3

    Plasmid

    #135464

    Purpose

    Bacterial expression of uTEV3 (full-length) protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    His6-MBP-uTEV3
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability) , I138T/S153N…

    Promoter

    T5

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-DR30(SapI)-EFS-CasRx-pA

    Plasmid

    #192488

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    EFS

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-Osp.gRNA-MS2in

    Plasmid

    #229772

    Purpose

    Contains the U6 promoter and an optimized gRNA backbone inserted with two copies of the MS2 stem loop.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

    Insert

    gRNA cassette with two MS2 stem loops
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-2NLS-Cas9

    Plasmid

    #229773

    Purpose

    Expresses Cas9 driven by a CMV promoter

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. DVK_pBR322_AE

    Plasmid

    #217592

    Purpose

    Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-pcDNA3.1 Puro-CAG-Positron2

    Plasmid

    #239079

    Purpose

    Mammalian expression of positive-going voltage sensor

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R78K N81D D92N W178F

    Promoter

    CAG

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tad-CBE(GCC3)

    Plasmid

    #235078

    Purpose

    Expresses Tad-CBE(GCC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCC3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tad-CBE(GCG4)

    Plasmid

    #235080

    Purpose

    Expresses Tad-CBE(GCG4) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCG4)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tad-CBE(ACC2)

    Plasmid

    #235061

    Purpose

    Expresses Tad-CBE(ACC2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. Sc RFC5-pCDFDuet

    Plasmid

    #239202

    Purpose

    Overexpress Sc RFC5 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    RFC5 (RFC5 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 8061-E31

    Plasmid

    #234268

    Purpose

    Gateway ORF Entry clone of mouse Smad1 with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    Smad1 (Smad1 Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBX_RPL7a_smRuby2_HA

    Plasmid

    #236087

    Purpose

    Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    RPL7a-smRuby2-HA (RPL7A Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAIP4

    Plasmid

    #233686

    Purpose

    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

    Depositor

    Christopher Bahl

    Insert

    Smt3 (SMT3 )
    

    Use

    Tags

    HisTag

    Expression

    Mutation

    Promoter

    T7

    Available Since

    April 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pEW108

    Plasmid

    #232823

    Purpose

    Expresses yeast compass complex in insect cells

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    

    Use

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    Set1(762-1080)

    Promoter

    polyhedrin promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAIP4_9C74

    Plasmid

    #234238

    Purpose

    Heterologous protein expression of variant of super folded GFP in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    9C74
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. SaDHFR-pNIC-Bsa4

    Plasmid

    #231039

    Purpose

    Expresses Staphylococcus aureus Dihydrofolate reductase under T7 with HIS tag

    Depositor

    Josh Beckham

    Insert

    SaDHFR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MbCas12a_N1153_GPG_GR2

    Plasmid

    #231578

    Purpose

    Encodes a cortisol inducible variant of MbCas12a

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    MbCas12a with a GR2 insertion behind N1153
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO.1-mTagBFP2-rMPC1 shRNA

    Plasmid

    #229016

    Purpose

    Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Rat)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 18065-M35-412

    Plasmid

    #225670

    Purpose

    Lentiviral protein expression of a TGF-beta pathway reporter

    Depositor

    Dominic Esposito

    Insert

    dsEGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    S3x6-JunBp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. scFv-HaloTag-iLID

    Plasmid

    #227390

    Purpose

    Adhesion module, scFv binds SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    scFv-HaloTag-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BRD4dN-mCherry-SspB

    Plasmid

    #227387

    Purpose

    Condensate module, light-inducible oligomerization IDR

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    BRD4 IDR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHR-SNAP-PDL1

    Plasmid

    #223602

    Purpose

    Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 Ras-LOCKR-PL Key

    Plasmid

    #216503

    Purpose

    Ras activity dependent proximity labeler, Key only, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-PL Key
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3 Ras-LOCKR-PL Cage

    Plasmid

    #216502

    Purpose

    Ras activity dependent proximity labeler, Cage only, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-PL Cage
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEM8731

    Plasmid

    #213991

    Purpose

    PrpsM driven expression of mNeonGreen - sequence codon-optimized for Salmonella

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGG-TurboID-GFP

    Plasmid

    #209399

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid

    #169524

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA3_PKA-RIIα-sREACh

    Plasmid

    #168489

    Purpose

    Mammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    PKA regulatory subunit IIα C-terminally tagged by sREACh
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC57-attB2-SA(2)-CRY2-mCherry

    Plasmid

    #160441

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge