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179,521 results

Showing: 9041 - 9080 of 179521 results

  1. pSLC-217

    Plasmid
    #73163
    Purpose
    Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.
    Depositor
    Swaine Chen
    Publication
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-relE (relE E. coli (bacteria))
    Expression
    Bacterial
    Promoter
    PrhaB
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73163-plasmid-map-sequence-id-151344

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  3. pZ8-T_dCas9

    Plasmid
    #74062
    Purpose
    pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR
    Depositor
    Timothy Lu
    Publication
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74062-plasmid-map-sequence-id-137972

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  6. pUBC_V5_MS2-mCherry

    Plasmid
    #68420
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2
    Use
    Synthetic Biology
    Tags
    V5 and mCherry
    Expression
    Mammalian
    Mutation
    MS2: V75E/A81G
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68420-plasmid-map-sequence-id-511194

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  8. MIP 227 CoMiP 4in1 wo shRNA p53

    Plasmid
    #63727
    Purpose
    To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Publication
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV
    Available since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63727-plasmid-map-sequence-id-114454

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  10. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  11. Str-KDEL_ManII-SBP-EGFP

    Plasmid
    #65252
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65252-plasmid-map-sequence-id-120582

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  12. pCAGGS-FuG-B2

    Plasmid
    #67513
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Kazuto Kobayashi
    Publication
    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.
    Insert
    Fusion Glycoprotein type B2
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67513-plasmid-map-sequence-id-381535

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  17. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Publication
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Tags
    None
    Expression
    Mammalian
    Promoter
    CMV and U6/H1
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

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  18. pUbiGUSPlus

    Plasmid
    #64402
    Purpose
    Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.
    Depositor
    Claudia Vickers
    Publication
    Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E
    Insert
    ubiquitin (Ubi-1) promoter
    Tags
    GUSPlus reporter
    Expression
    Plant
    Promoter
    Ubi-1
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64402-plasmid-map-sequence-id-108183

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  20. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64710-plasmid-map-sequence-id-108923

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  24. pMF440

    Plasmid
    #62550
    Purpose
    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
    Depositor
    Michael Franklin
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    trc
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62550-plasmid-map-sequence-id-397042

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  27. PX852

    Plasmid
    #62884
    Purpose
    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62884-plasmid-map-sequence-id-105906

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  28. PX851

    Plasmid
    #62883
    Purpose
    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62883-plasmid-map-sequence-id-105905

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  29. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

    Plasmid
    #52889
    Purpose
    "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression
    Depositor
    Iswar Hariharan
    Publication
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Insert
    Gal4 (GAL4 Budding Yeast)
    Expression
    Insect
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52889-plasmid-map-sequence-id-99556

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  30. mTagBFP-Nucleus-7

    Plasmid
    #55265
    Purpose
    Localization: Nuclear Localization, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mTagBFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    55265-plasmid-map-sequence-id-475376

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  31. mCherry-FilaminA-N-9

    Plasmid
    #55047
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FilaminA (FLNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    D2626H
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  32. mCherry-Alpha-5-Integrin-12

    Plasmid
    #54970
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Alpha-5 Integrin (ITGA5 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54970-plasmid-map-sequence-id-218701

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Showing: 9041 - 9080 of 179521 results