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Showing: 11301 - 11350 of 32274 results

  3. shNTC

    Plasmid
    #73549
    Purpose
    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).
    Depositor
    Gerardo Ferbeyre
    Article
    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d
    Insert
    shNTC
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pPotef-cdc3CB16-NatR

    Plasmid
    #86465
    Purpose
    Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.
    Depositor
    Michael Feldbrügge
    Article
    Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10
    Insert
    Cdc3
    Use
    Ustilago maydis expression
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Potef
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    86465-plasmid-map-sequence-id-173986

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  17. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid
    #22931
    Purpose
    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)
    Depositor
    Alexandra Newton
    Article
    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.
    Insert
    PHLPP1-deltaC (PHLPP1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    PHLPP-deltaC, deletion of PDZ binding domain--the…
    Promoter
    CMV
    Available since
    Feb. 4, 2010
    Availability
    Academic Institutions and Nonprofits only

  18. pDBEcR/Bla

    Plasmid
    #201556
    Purpose
    Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201556-plasmid-map-sequence-id-400918

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  26. Tet-FRNK-GFP

    Plasmid
    #83472
    Purpose
    Lentiviral vector for doxycycline-inducible expression of FRNK in mammalian cells
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    FRNK (PTK2 Chicken)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    FRNK is the non-catalytic c-term domain of FAK ki…
    Promoter
    doxycycline-inducible promoter
    Available since
    Oct. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    83472-plasmid-map-sequence-id-159108

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  27. pQE80l B-CN-B

    Plasmid
    #196477
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
    Depositor
    Fei Sun
    Article
    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1
    Insert
    B-CN-B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196477-plasmid-map-sequence-id-387241

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  34. dCas9-NLS-gs10-cGR2176

    Plasmid
    #188258
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cGR2176
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188258-plasmid-map-sequence-id-377988

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  41. pJK503

    Plasmid
    #155380
    Purpose
    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    May 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    155380-plasmid-map-sequence-id-309009

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  43. pICE-EGFP-FLAG-Ku70siR-WT

    Plasmid
    #46961
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    human Ku70 (XRCC6 Human)
    Use
    Tags
    GFP-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Tet
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46961-plasmid-map-sequence-id-66851

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Showing: 11301 - 11350 of 32274 results