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20,201 results

Showing: 9201 - 9240 of 20201 results

  14. pCKmatBC

    Plasmid
    #138587
    Purpose
    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    matB contains native GUG start codon
    Promoter
    Plac promoter
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138587-plasmid-map-sequence-id-274478

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  15. pYC1640

    Plasmid
    #158719
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158719-plasmid-map-sequence-id-320840

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  27. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  31. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available Since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  32. pENN.AAV.EF1a.eGFP.WPRE.rBG

    Plasmid
    #105547
    Purpose
    AAV expression of EGFP from EF1a promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV8
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105547-plasmid-map-sequence-id-202477

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Showing: 9201 - 9240 of 20201 results