We narrowed to 2,568 results for: PGK
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Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W
Plasmid#200504PurposeLentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2DepositorAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)
Plasmid#215009PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-Fluc-PGK-Rluc-mCherry
Plasmid#191863PurposeHeat inducible Fluc reporter with constitutive RlucDepositorInsertsFirefly luciferase Fluc
Renilla luciferase Rluc
UseLentiviralTagsP2A-mCherryExpressionMammalianPromoterHSPA7 promoter and PGK promoterAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-NLS-tdTomato-PGK-Puro
Plasmid#89993PurposeDonor template for generation of SOX9-ntdTomato reporter cell linesDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-GFP-PGK-Puro
Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
Plasmid#52549Purposetransgenic construct for expression of GFP and FlpoDepositorInsertsPhiC31o attB (full length)
GFP
Flpo recombinase
ExpressionMammalianAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP
Plasmid#195503PurposeSOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locusDepositorInsertSOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
UseCRISPR; Donor vectorTagsT2A-H2B-mCitrineExpressionMammalianAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-F2P-LoxP-PGK-PuroR-LoxP
Plasmid#227502PurposeInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassetteDepositorInsertPrdm8 promoter
UseAAV and Cre/LoxAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
Plasmid#118155PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNTK human MBD3 PGK-Neo-pA flox
Plasmid#52362PurposeTargeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.DepositorInsertMBD3 (MBD3 Human)
UseTargetingAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQR-P2A-GFP-PGK-Puro
Plasmid#110861PurposeLentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)DepositorInsertCas9-VQR
UseLentiviralTags3X FLAGMutationD1135V, R1335Q, T1337RPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9-PGK-Puro-IRES-GFP
Plasmid#75114PurposeRetroviral expression plasmid encoding wild type human BRD9DepositorInsertBRD9 (BRD9 Human)
UseRetroviralAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
FH1-CLGT#2-mCherry-_PGK-H2B-CFP
Plasmid#164106PurposeFluorescent reporter for genetic tracing of classical Glioblastoma cell-state.DepositorInsertCLGT#2-mCherry
UseLentiviral and Synthetic BiologyAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-LC3B G120A
Plasmid#123242PurposeExpression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterPGKAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast
Plasmid#205549PurposeExpress EPN24-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-3xFLAG-empty-pgk-hph
Plasmid#48754PurposePiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tagDepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterCAGAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT D554N (Puro)
Plasmid#154292PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationD554N mutation (Ser/Thr glycosylation-incompetent)PromoterPGKAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF3-3xHA-pGK-Pur
Plasmid#69053Purposelentiviral expression of IKZF3 with HA tagDepositorAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116
Plasmid#123243PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterPGKAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Tfe3::ERT2-pgk-hph
Plasmid#48756PurposePiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selectionDepositorAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR
Plasmid#126697Purposedonor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertTagBFP
ExpressionMammalianAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-miRFP670nano-PGK-Blast
Plasmid#196722PurposeTet-inducible expression of Cas9-T2A-miRFP670nanoDepositorInsertCas9-T2A-miRFP670nano
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSPromoterTRE, PGKAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)
Plasmid#194229PurposeLentiviral expression vector expressing LCB1 SynNotch and mCherry transduction markerDepositorInsertPGK-LCB1-SynNotch-T2A-mCherry
UseLentiviralPromoterPGKAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
Plasmid#75122PurposeRetroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tagDepositorAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-3xFLAG::Flcn-pgk-hph
Plasmid#48757PurposePiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selectionDepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_dBD-PGK-Puro-IRES-GFP
Plasmid#75115PurposeRetroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomainDepositorAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA
Plasmid#140221PurposeConstitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipperDepositorInsertdLbCpf1 (Cas12a) N terminal half (406) with zipper
ExpressionMammalianPromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-Blast
Plasmid#44245DepositorAvailable SinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT 5N5A (Puro)
Plasmid#154293PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationN322A, N356A, N390A, N424A, N458A mutations; HCF-…PromoterPGKAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pGK-nAChRb2(E61C)-IRES-GFP
Plasmid#164777Purposecysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligandDepositorInsertnAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
UseLentiviralExpressionMammalianMutationE61CPromoterpGKAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-CRBN-pGK-HYG
Plasmid#107376PurposeLentiviral expression of myc-CRBNDepositorAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE
Plasmid#89607PurposeExpression of LIN28A in human cellsDepositorAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur
Plasmid#69051Purposelentiviral expression of IKZF1 with HA tagDepositorInsertIKZF1 (IKZF1 Human)
UseLentiviralTagsHAExpressionMammalianMutationsplice variant 2PromoterUBCAvailable SinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-CDK6-mutPAM-PGK-puro
Plasmid#187638Purposesg resistant CDK6 ORFDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-221-PGK-GFP-Blast
Plasmid#31519DepositorAvailable SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
N111 nLV EF-1a-PGK-Puro Gal4-HP1a FL
Plasmid#43968DepositorAvailable SinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
N113 nLV EF-1a-Gal4-Stop-PGK-Puro
Plasmid#44176DepositorInsertGal4/DBD (1-147)
UseLentiviralPromoterEF-1aAvailable SinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry
Plasmid#75132PurposeRetroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-GFP-PGK-Puro
Plasmid#110868PurposeLentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralMutationD10APromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-V12-puro
Plasmid#156168PurposeLentiviral vector for expression of flag-RAP1B-V12, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP
Plasmid#75118PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomainDepositorAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP
Plasmid#75129PurposeRetroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-3xFLAG::Fnip1-pgk-hph
Plasmid#48758PurposePiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selectionDepositorAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)
Plasmid#71213PurposedCas9 fused to BFP and the human DNMT3A catalytically inactive domainDepositorInsertdSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
TagsdSpCas9-BFP-DNMT3A(E752A)ExpressionMammalianAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagMutationbromodomain replaced with the first bromodomain o…Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Flag3-pG-mNanog-pgk-hph
Plasmid#74911PurposeMammalian expression of Flag tagged mNanogDepositorAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode1
Plasmid#83678Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode1
UseRetroviralAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo
Plasmid#74919PurposeMammalian expression of mKlf2 and mNanog-mCherryDepositorTagsT2A and mCherryExpressionMammalianPromoterCAGAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only