Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

161,838 results

Showing: 9561 - 9600 of 161838 results

  3. pCS2-UTX-F-MT2

    Plasmid
    #40619
    Purpose
    Expression of enzyme-dead UTX in mammalian cells
    Depositor
    Kai Ge
    Article
    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.
    Insert
    UTX (KDM6A Human)
    Use
    Tags
    6xMyc and Flag
    Expression
    Mammalian
    Mutation
    changed Histidine 1146 and Glutamic Acid 1148 to …
    Promoter
    CMV
    Available since
    Nov. 5, 2012
    Availability
    Academic Institutions and Nonprofits only

  7. psfTn5

    Plasmid
    #79107
    Purpose
    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations
    Depositor
    Sten Linnarsson
    Insert
    transposase 5
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E54K and L372P
    Promoter
    Available since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    79107-plasmid-map-sequence-id-418885

    Enlarge

  8. ZJOM119

    Plasmid
    #133661
    Purpose
    Integration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    133661-plasmid-map-sequence-id-265756

    Enlarge

  10. PSD-95-pTagRFP

    Plasmid
    #52671
    Purpose
    expression of TagRFP-tagged PSD-95
    Depositor
    Johannes Hell
    Insert
    PSD-95 (Dlg4 Rat)
    Use
    Tags
    tagRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52671-plasmid-map-sequence-id-272433

    Enlarge

  12. pRS413-GAL1-yEGFP1

    Plasmid
    #40235
    Depositor
    Michael Benton
    Insert
    yEGFP1
    Use
    Tags
    HIS3MX6
    Expression
    Bacterial
    Mutation
    Promoter
    GAL1
    Available since
    Oct. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    40235-plasmid-map-sequence-id-51356

    Enlarge

  13. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Article
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Insert
    human progesterone receptor-B (PGR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89130-plasmid-map-sequence-id-176077

    Enlarge

  17. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Use
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111848-plasmid-map-sequence-id-213454

    Enlarge

  20. pD649-HAsp-ROBO2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156991
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ROBO2 (ROBO2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156991-plasmid-map-sequence-id-412875

    Enlarge

  21. pcDNA3-TLR2-CFP

    Plasmid
    #13015
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13015-plasmid-map-sequence-id-471621

    Enlarge

  22. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

    Enlarge

  28. AICSDP-47: CTNNB1-mEGFP

    Plasmid
    #109119
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1
    Depositor
    Allen Institute for Cell Science
    Insert
    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109119-plasmid-map-sequence-id-209765

    Enlarge

Showing: 9561 - 9600 of 161838 results