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20,128 results

Showing: 7531 - 7560 of 20128 results

  1. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available Since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    62887-plasmid-map-sequence-id-105910

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  3. pCMV5 HA-TGFBRII

    Plasmid
    #19158
    Depositor
    Joan Massague
    Insert
    Transforming growth factor, beta receptor II (TGFBR2 Human)
    Tags
    HA (YDVPDYASL)
    Expression
    Mammalian
    Mutation
    PCR based mutagenesis
    Available Since
    Oct. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    19158-plasmid-map-sequence-id-364716

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  19. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Expression
    Bacterial
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  21. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid
    #171385
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized minidCas13X.1-RESCUE-S
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171385-plasmid-map-sequence-id-337353

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  23. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  26. pOpen-T5gene12/13

    Plasmid
    #165559
    Purpose
    Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T5 Exonuclease
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165559-plasmid-map-sequence-id-339952

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  29. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Promoter
    pDAB
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

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Showing: 7531 - 7560 of 20128 results