Skip to main content
Addgene
Showing: 10801 - 10840 of 23940 results

  5. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

    Enlarge

  10. pKS diaCas9_sgRNA

    Plasmid
    #74923
    Purpose
    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter
    Depositor
    Per Winge
    Article
    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Cas9 module and sgRNA module
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74923-plasmid-map-sequence-id-149703

    Enlarge

  16. pAC-ZEAX

    Plasmid
    #53274
    Purpose
    Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52
    Insert
    crtE, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Deleted idi (ORF6) and crtX genes
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53274-plasmid-map-sequence-id-83289

    Enlarge

  33. pCIP-caTfeb

    Plasmid
    #79013
    Purpose
    For mammalian expression of a shortened, constitutively active version of Tfeb.
    Depositor
    Reuben Shaw
    Article
    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:
    Insert
    Tfeb (TFEB Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Removed first ~400 bp from 5' end (N-terminu…
    Promoter
    pCAGGs
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  37. pMBP-SpyCatcher

    Plasmid
    #79053
    Purpose
    Expresses maltose binding protein (MBP) SpyCatcher fusion
    Depositor
    James Bowie
    Article
    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi
    Insert
    MBP-SpyCatcher
    Use
    Tags
    6xHis and Maltose binding protein
    Expression
    Bacterial
    Mutation
    Derived from pDEST14-SpyCatcher (Plasmid #35044)…
    Promoter
    T7
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    79053-plasmid-map-sequence-id-147896

    Enlarge

  39. pFUGW-hGtACR2-EYFP

    Plasmid
    #67877
    Purpose
    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
    Depositor
    John Spudich
    Article
    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.
    Insert
    hGtACR2
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67877-plasmid-map-sequence-id-199004

    Enlarge

Showing: 10801 - 10840 of 23940 results