Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

179,521 results

Showing: 11361 - 11400 of 179521 results

  5. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid
    #104491
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7s
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104491-plasmid-map-sequence-id-200288

    Enlarge

  6. pGART-GFP

    Plasmid
    #99106
    Purpose
    Expresses human GART-GFP fusion in mammalian cells
    Depositor
    Stephen Benkovic
    Publication
    An et al Science. 2008 Apr 4;320(5872):103-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GART (GART Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    A65T, L68V, and A72S mutations in GFP gene; kanR/…
    Promoter
    CMV
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99106-plasmid-map-sequence-id-200317

    Enlarge

  8. mTurquoise2-LaminB

    Plasmid
    #98830
    Purpose
    In vivo visualization of the nuclear envelope (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Publication
    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LaminB
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    *(see below)
    Promoter
    CMV
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98830-plasmid-map-sequence-id-190907

    Enlarge

  15. pmCherry-N1-GalT

    Plasmid
    #87327
    Purpose
    To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Publication
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87327-plasmid-map-sequence-id-177633

    Enlarge

  21. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Publication
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

    Enlarge

  28. NPY-pHTomato

    Plasmid
    #83501
    Purpose
    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.
    Depositor
    Sebastian Barg
    Publication
    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NPY (NPY Human)
    Tags
    pHTomato
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83501-plasmid-map-sequence-id-163242

    Enlarge

  31. pZA16mflon

    Plasmid
    #75439
    Purpose
    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
    Depositor
    James Collins
    Publication
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    mf-lon
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75439-plasmid-map-sequence-id-147407

    Enlarge

  33. MELOPS

    Plasmid
    #80151
    Purpose
    Genetically encoded melanosome-localized pH sensor.
    Depositor
    Santiago Di Pietro
    Publication
    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OCA2 (OCA2 Human)
    Tags
    mNectarine in first luminal loop
    Expression
    Mammalian
    Mutation
    nonpathologic polymorphism D257A, pore mutation V…
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  34. Hs.NRAS G12D

    Plasmid
    #83176
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    G12D
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83176-plasmid-map-sequence-id-158139

    Enlarge

  39. pcDNA5-FRT-TO-AID-EGFP

    Plasmid
    #80076
    Purpose
    Mammalian expression vector for C-terminal AID-EGFP tagging
    Depositor
    Andrew Holland
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-EGFP
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80076-plasmid-map-sequence-id-150336

    Enlarge

Showing: 11361 - 11400 of 179521 results