161,439 results

1. pCMV-Neo-Bam

   Plasmid

   #16440

   Purpose

   Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. σ1-mCherry

   Plasmid

   #186578

   Purpose

   Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP1S1 (AP1S1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Eps15-pmCherryN1

   Plasmid

   #27696

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Eps15 (EPS15 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-mEGFP-CaMKIIa

   Plasmid

   #127389

   Purpose

   Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 1013 pcDNA3 T7 Akt1 T308A S473A

   Plasmid

   #9030

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 T308A S473A (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T308A S473A

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. IDG_TMEM63B_OE_1

   Plasmid

   #161682

   Purpose

   Overexpresses TMEM63B

   Depositor

   Mike McManus

   Insert

   TMEM63B (TMEM63B Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR4 no ccDB (686-1)

   Plasmid

   #17424

   Purpose

   Gateway entry vector, no ccDB gene

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Puro-mGSDME

   Plasmid

   #154877

   Purpose

   Expresses mGSDME in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (Gsdme Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDZ Flag USP28

   Plasmid

   #15665

   Depositor

   Martin Eilers

   Article

   Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

   Insert

   USP28 (USP28 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pET21a-CysRS-His

    Plasmid

    #124107

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Cysteinyl-tRNA ligase (cysS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DsRed-rab11 WT

    Plasmid

    #12679

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdEasy-2

    Plasmid

    #16401

    Purpose

    Adenoviral vector for use in AdEasy System, for use with large inserts.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pORANGE Dlg4-GFP KI

    Plasmid

    #131477

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Dlg4, Dlg4 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-FOXM1

    Plasmid

    #153136

    Purpose

    Expression of Flag tagged human FOXM1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXM1 (FOXM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-lyso-pHoenix

    Plasmid

    #70112

    Purpose

    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHoenix (Cd63 Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse)
    

    Use

    Tags

    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSBbi-GB

    Plasmid

    #60520

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a-HisRS-His

    Plasmid

    #124111

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Histidyl-tRNA ligase (hisS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Brachyury-eGFP

    Plasmid

    #21063

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-PE2-C

    Plasmid

    #164908

    Purpose

    Expresses the C-terminal split-intein fragment of PE2

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    PE2-C
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-SET-FLAG-HA

    Plasmid

    #24998

    Depositor

    Judy Lieberman

    Article

    Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

    Insert

    SET (SET Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-TBG-Cyto-TurboID

    Plasmid

    #149414

    Purpose

    Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    Cyto-TurboID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mutation

    Promoter

    TBG

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCerulean-VSVG

    Plasmid

    #11913

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-Htr5b(3.7)-tTA-sv40

    Plasmid

    #184412

    Purpose

    AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-tTA
    

    Use

    AAV

    Tags

    tTA

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMemoryArray

    Plasmid

    #60585

    Purpose

    An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    MemoryArray
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-mGFP-Actin

    Plasmid

    #21948

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

    Insert

    betaActin (ACTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEL685

    Plasmid

    #205592

    Purpose

    NEPE for prime editing in rice

    Depositor

    Yong Zhang

    Article

    Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ZmUbi

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-DeltaJunD

    Plasmid

    #68547

    Purpose

    AAV expression of DeltaJunD

    Depositor

    Eric Nestler

    Article

    Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

    Insert

    DeltaJunD-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant

    Promoter

    CMV

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

29. TFORF2700

    Plasmid

    #142978

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESRRG (ESRRG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAV-U6+27-Tornado-BroccoliSamSensor#1

    Plasmid

    #124363

    Purpose

    Overexpression of circular RNA (Broccoli-based SAM sensor)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-BroccoliSamSensor#1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLPT107

    Plasmid

    #85525

    Purpose

    Repressilator with triple reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    mKate2
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Ac5-STABLE2-neo

    Plasmid

    #32426

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    Flag-mCherry-T2A-GFP-T2A-neo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC18T-mini-Tn7T-Gm-eyfp

    Plasmid

    #65031

    Purpose

    GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 HA-humanTRAF2 (1084)

    Plasmid

    #66932

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-LKB1-KM

    Plasmid

    #21148

    Depositor

    Junying Yuan

    Article

    Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

    Insert

    GFP-LKB1-KM (Stk11 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K78M

    Promoter

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

36. GFP-hPIKfyve

    Plasmid

    #121148

    Purpose

    Expresses GFP-tagged human PIKfyve in mammalian cells.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

    Insert

    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-hSyn Coff/FonEYFP

    Plasmid

    #55652

    Purpose

    Cre-off/Flp-on EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre off/flp on eyfp

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mCherry-Parkin-IRES-zeo

    Plasmid

    #61727

    Purpose

    Expression of human Parkin fused to mCherry

    Depositor

    Douglas Green, Stephen Tait

    Article

    Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

39. R701-X65-527: His6-tev-Hs.CALM1(2-148)

    Plasmid

    #159693

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(2-148)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(2-148) (CALM1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. dimericTomato-1xwGBD

    Plasmid

    #176099

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WAS (WAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge