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Showing: 1121 - 1160 of 157401 results

  9. TRE-mClover3-TDP-43ΔNLS

    Plasmid
    #214917
    Purpose
    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta
    Depositor
    Ophir Shalem
    Article
    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d
    Insert
    TDP-43ΔNLS (TARDBP Human)
    Use
    Lentiviral
    Tags
    mClover3
    Expression
    Mutation
    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214917-plasmid-map-sequence-id-424953

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  14. T7-VEE-GFP

    Plasmid
    #58977
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58977-plasmid-map-sequence-id-419580

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  15. pAAV2/2

    Plasmid
    #104963
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 2
    Depositor
    Melina Fan
    Insert
    Rep2/Cap2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p5
    Available since
    Oct. 2, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    104963-plasmid-map-sequence-id-372962

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  17. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid
    #139997
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139997-plasmid-map-sequence-id-352446

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  18. mCherry-Ezrin-N-14

    Plasmid
    #55043
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Ezrin (EZR Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55043-plasmid-map-sequence-id-271041

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  23. pLXSN16E6E7

    Plasmid
    #52394
    Purpose
    The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.
    Depositor
    Denise Galloway
    Article
    Halbert et al J Virol. 1991 Jan;65(1):473-8.
    Insert
    HPV16 E6E7
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    52394-plasmid-map-sequence-id-371138

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  25. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid
    #197507
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197507-plasmid-map-sequence-id-386698

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  28. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

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  30. TFORF1932

    Plasmid
    #142292
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    JUN (JUN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142292-plasmid-map-sequence-id-395770

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  32. TFORF0707

    Plasmid
    #143889
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR1 (NCOR1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    143889-plasmid-map-sequence-id-408610

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  33. pYC-C

    Plasmid
    #207114
    Purpose
    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
    Depositor
    Yifan Cheng
    Article
    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207114-plasmid-map-sequence-id-414780

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  36. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    113865-plasmid-map-sequence-id-220242

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  37. PLJR965

    Plasmid
    #115163
    Purpose
    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Sarah Fortune
    Article
    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:
    Inserts
    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    115163-plasmid-map-sequence-id-222098

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Showing: 1121 - 1160 of 157401 results