We narrowed to 17,377 results for: puro

1. pMXs-puro GFP-p62 dC

   Plasmid

   #38282

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncate mutant 1-265 amino acids residues

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. N-Myc-53BP1 WT pLPC-Puro

   Plasmid

   #19836

   Depositor

   Titia de Lange

   Article

   Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

   Insert

   p53 Binding Protein 1 (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMKO.1 puro hTERT shRNA

   Plasmid

   #10688

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   hTERT shRNA (TERT Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-N-Puro-moxGFP-LMNB1

   Plasmid

   #207773

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. qTAG-N-Puro-mNeon-ACTB

   Plasmid

   #207752

   Purpose

   Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTet-O-NEUROD1-T2A-PuroR

   Plasmid

   #162338

   Purpose

   Lentiviral expression of NEUROD1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 1066 pBabe puroL PTEN

   Plasmid

   #10785

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eSpCas9(1.1)-T2A-Puro

   Plasmid

   #101039

   Purpose

   Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

   Depositor

   Andrea Németh

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFLAG and T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1 puro shRNA E-cadherin

   Plasmid

   #18801

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   E-cadherin shRNA (CDH1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 22, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid

    #207758

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pKLV2-U6gRNA5(SMARCA4(15))-PGKpuro2ABFP-W

    Plasmid

    #200462

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(15) (SMARCA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-U6gRNA5(SMARCA4(43))-PGKpuro2ABFP-W

    Plasmid

    #200464

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43) (SMARCA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-surfaceHA-IRES-Puro

    Plasmid

    #207321

    Purpose

    Lentiviral expression of synthetic HA surface receptor in Mammalian cells

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    HAtag-Myctag-PDGFRB-TM-IRES-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha_short (EFS)

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTet-O-LHX8-T2A-PuroR

    Plasmid

    #162350

    Purpose

    Lentiviral expression of LHX8 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    LHX8 (LHX8 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABE 3xHA-MKK6-EE puro

    Plasmid

    #216791

    Purpose

    Mammalian expression of constitutively active human MKK6

    Depositor

    Kevin Janes

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Retroviral

    Tags

    3x-HA

    Expression

    Mammalian

    Mutation

    S207E and T211E, constitutively active

    Promoter

    LTR

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO-puro-tetON-shRFP

    Plasmid

    #110940

    Purpose

    Tet-inducible shRNA targeting RFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shRFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE puro PPAR gamma 2

    Plasmid

    #8859

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCVpuro-Flag-mAIM2-EGFP

    Plasmid

    #51667

    Purpose

    Express Flag and GFP-tagged mAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    MESV

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-R2L3-IRES-puro-pA

    Plasmid

    #139529

    Purpose

    Provides IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    puromycin resistant gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCW-Cdt1-mCherry-Puro

    Plasmid

    #193763

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    WT

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMMLV-mRFP1-P2A-Puro

    Plasmid

    #160228

    Purpose

    Expresses mRFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    mRFP1-P2A-Puro
    

    Use

    Retroviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. qTAG-N-Puro-moxGFP-TUBA1B

    Plasmid

    #207767

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

    Plasmid

    #148859

    Purpose

    Mammalian Expression of HsXRN1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsXRN1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLentiPGK Puro DEST JNKKTRmCerulean3

    Plasmid

    #90232

    Purpose

    Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Ki67∆Nterm-mNeonGreen-IRESpuro2

    Plasmid

    #183740

    Purpose

    Expresses Ki67∆Nterm-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + all Ki-67 repeats (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-TetOne-Puro-MDM4

    Plasmid

    #195140

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCW-Cdt1ΔPIP-mCherry-Puro

    Plasmid

    #193765

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1ΔPIP-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    PIP degron removed (a.a.1-19 removed)

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

    Plasmid

    #112837

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SH2 + SH3 (C-term) domains of Grb2

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBabe-puro-miR-9 sponge

    Plasmid

    #25040

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    miR-9 sponge
    

    Use

    Retroviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSCVpuro-Flag-cMyc T58A

    Plasmid

    #20076

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQC V5 MCS IRES Puro

    Plasmid

    #110342

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSBbi-RP-CoV2/RBD-puro

    Plasmid

    #161793

    Purpose

    Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

    Insert

    SARS-CoV-2 RBD
    

    Use

    Transposon

    Tags

    6xHis and dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLC-Flag-CSNK1A1 WT-Puro

    Plasmid

    #123319

    Purpose

    Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

    Plasmid

    #218666

    Purpose

    sgRNA targeting human EIF2AK3/PERK

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-TET3G-T2A-Puro

    Plasmid

    #158067

    Purpose

    Encodes the Tet3G transactivator and puromycin resistance.

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    pCAG-TET3G-T2A-Puro
    

    Use

    AAV

    Tags

    puromycin reistance gene

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid

    #137730

    Purpose

    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TetO-FUW-pgk-puro

    Plasmid

    #85747

    Purpose

    tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

    Depositor

    Emily Dykhuizen

    Article

    Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    none

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLKO.1-puro U6 sgRNA CAG

    Plasmid

    #50927

    Purpose

    U6 driven sgRNA negative control

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    negative control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Use

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP-PCNA-IRES-puro2b

    Plasmid

    #26461

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    pEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLKO.1-puro-Nono-sh1

    Plasmid

    #127650

    Purpose

    Knock-down of human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO shRNA (NONO Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLKO.1-puro-Nono-sh5

    Plasmid

    #127651

    Purpose

    Knock-down of human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO shRNA (NONO Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pPOTv6-puro-3Myc:mNG:3Myc

    Plasmid

    #179803

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean, Keith Gull

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mNG
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. MSCV-puro-N3FLAG-MYB-FL

    Plasmid

    #105596

    Purpose

    retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Full length MYB (mouse) (Myb Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

    Plasmid

    #218528

    Purpose

    sgRNA targeting human EIF2AK4/GCN2

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid

    #48226

    Purpose

    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    

    Use

    CRISPR

    Tags

    2A-puro, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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