We narrowed to 13,707 results for: cas9

1. pH-dCas9-PBE

   Plasmid

   #98162

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-dCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p1195-pssAAV.U1a.hNme2Cas9

   Plasmid

   #129534

   Purpose

   AAV vector expressing Nme2Cas9

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Insert

   human codon-optimized Nme2Cas9
   

   Use

   AAV

   Tags

   2xNLS and NLS-3xHA-NLS

   Expression

   Mutation

   Promoter

   U1a

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO5d.EFS.SpCas9.P2A.PAC

   Plasmid

   #58329

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

   Plasmid

   #81251

   Purpose

   Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pB-dCas9-PBE

   Plasmid

   #98159

   Purpose

   Expresses CRISPR base editor in maize

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-dCas9-UGI-NLS
   

   Use

   Maize

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ-dpcoCas9-TV

   Plasmid

   #158409

   Purpose

   CRISPR-dpcoCas9 fused with TV activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-TV(6xTAL-2xVP64)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas9-beta

   Plasmid

   #169240

   Purpose

   Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

   Plasmid

   #110624

   Purpose

   A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   LP1B and hU6

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BB1_23_dCAS9(3xFLAG_SV40NLS)

    Plasmid

    #161478

    Purpose

    BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Insert

    dCAS9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    FLAG Tag, SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX165-eSpCas9

    Plasmid

    #140564

    Purpose

    Expresses eSpCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    K848A K1003A R1060A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SauCas9 PE2*

    Plasmid

    #169851

    Purpose

    Mammalian Expression, sauCas9 based prime editor

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SaCas9-N580A-M-MLV
    

    Use

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV-dCas9_puroR

    Plasmid

    #219825

    Purpose

    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    CMV

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eSpCas9-EF-5Nanog_2

    Plasmid

    #174871

    Purpose

    CRISPR vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330A_dCas9-1x2

    Plasmid

    #63596

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SaCas9v3-Puro

    Plasmid

    #178813

    Purpose

    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMonAID_nCas9-PmCDA_Hyg_ALS

    Plasmid

    #91693

    Purpose

    Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge