170,806 results
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Plasmid#164107PurposeDetect the long Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Long P2A
ExpressionMammalianAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Blast
Plasmid#83480PurposeMammalian expression of Cas9 and sgRNA scaffoldDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterEF-1αAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-YFP
Plasmid#18826DepositorAvailable SinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
psfaRb-Tn5
Plasmid#183638PurposeExpresses anti-rabbit nanobody fused with Tn5DepositorInsertanti rabbit secondary nanobody fused to Tn5
ExpressionBacterialAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDawn-sfGFP
Plasmid#107741PurposeExpresses superfolder GFP under the control of a blue-light transcriptional regulatorDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
WT GBA pcDNA3.1
Plasmid#188580PurposeEctopic expression of wild-type GBADepositorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-mOrange2
Plasmid#85212PurposeLentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoterDepositorInsertmOrange2
UseCre/Lox and LentiviralExpressionMammalianPromoterSFFVAvailable SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K63
Plasmid#17606PurposeMammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK63 only, other lysines mutated to arginines. Enh…Available SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XHA-EGFP-OMP25
Plasmid#83356PurposeFor tagging mitochondria with HA epitopes (HA-MITO construct)DepositorInsert3XHA-EGFP-OMP25
UseRetroviralExpressionMammalianAvailable SinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-luciferase
Plasmid#55764Purposeluciferase reporter for in vivo bioluminescence imagingDepositorInsertCMV enhancer/chicken beta actin promoter
ExpressionMammalianPromoterCMV enhancer/chicken beta actin promoterAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD1
Plasmid#20183DepositorInsertNotch 1 Intracellular Domain (Notch1 Mouse)
Tags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKC107_pLenti_TetON_sfGFP_INT
Plasmid#242109PurposeTet- or Dox-inducible expression of sfGFP fused to INT (human PARP4 fragment)DepositorInsertPARP4 fragment
UseLentiviralTagssfGFPMutationPARP4 aa 1562-1724PromoterTRE3GAvailable SinceDec. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6, mU6Available SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm
Plasmid#63121Purposemini-Tn7 vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV2/8
Plasmid#112864PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap8
UseAAVPromoterTruncated P5Available SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Puro (658-5)
Plasmid#17448Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, PuroDepositorHas ServiceCloning Grade DNAInsertGFP
UseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
ExpressionBacterialPromoternative phage promotersAvailable SinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K48
Plasmid#17605PurposeMammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK48 only, other lysines mutated to arginines. Enh…Available SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
TagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 light chain
Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
TagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianPromoterCMVAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG1/κ
Plasmid#61883PurposeExpresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Basic Vector
Plasmid#212936PurposeFirefly luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseMutationWTAvailable SinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Neo empty (w215-1)
Plasmid#17485Purpose3rd gen lentiviral empty control vector, CMV/TO promoter, NeoDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBBR1MCS-2
Plasmid#85168PurposeMobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneExpressionBacterialPromoterConstitutive (derepressed P-lac)Available SinceNov. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKC104_pLenti_TetON_MVP
Plasmid#242111PurposeTet- or Dox-inducible expression of MVPDepositorAvailable SinceDec. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHSP02
Plasmid#117259PurposeGenome editing for Lactobacillus plantarum WCSF1DepositorInsertCas9, promotor P11-guide-RNA, homologous arms of Lp_0537
UseOtherAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)
Plasmid#228881PurposePiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.DepositorInsertBuilding blocks of GEMINI for intracellular assembly
ExpressionMammalianPromoterTREAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
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YY206: pAAV_hSyn::GEMINI(B)-p2A-GEMINI(A)_UbC::HaloTag-GEMINI(A)
Plasmid#228886PurposeAAV plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a hSyn promoter, and HaloTag-GEMINI(A) under the control of a UbC promoter.DepositorInsertGEMINI(A); GEMINI(B); HaloTag-GEMINI(A)
UseAAVTagsHaloTagExpressionMammalianPromoterhSyn; UbcAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX330 p53
Plasmid#59910PurposepX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoterDepositorAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTn Donor
Plasmid#236211PurposeArabinose-inducible Tn5 InducTn-seq plasmidDepositorInsertNone
ExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G
Plasmid#194457PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV2A
Plasmid#224440PurposeRep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDQTTL insert between amino acids 588 and 589 of…Promoterp41Available SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralExpressionMammalianPromoterEF1 and PGKAvailable SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-PE7 for IVT
Plasmid#214813PurposeTemplate for in vitro transcription of PE7DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSPromoterT7 (inactivated)Available SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationKO: all lysines mutated to argininesAvailable SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET29b-10xHis-Super TEV
Plasmid#193833PurposeExpresses high-efficiency TEV protease in bacterial cellsInsertTEV protease
Tags6xHisExpressionBacterialMutationT17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…Available SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only