We narrowed to 16,819 results for: IGH@

1. pGWB553

   Plasmid

   #74883

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB550

   Plasmid

   #74880

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJWV102-PL-dCas9

   Plasmid

   #85588

   Purpose

   Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

   Insert

   dCas9sp
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLsp

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

   Plasmid

   #193049

   Purpose

   Encodes G-418 resistance

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   prsp-27::NeoR::unc-54 3’ UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   prsp-27

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

   Plasmid

   #114595

   Purpose

   Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

   Depositor

   Brian Chow

   Article

   Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

   Insert

   BcLOV4
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREDIA

   Plasmid

   #51625

   Purpose

   E. coli genome modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRha

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB445

   Plasmid

   #74831

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. a1Ic-HE3-pcDNA3

   Plasmid

   #45810

   Depositor

   Edward Perez-Reyes

   Article

   Gomora et al Biophys J. 2002 Jul;83(1):229-41.

   Insert

   CACNA1I (CACNA1I Human)
   

   Use

   Making crna for oocyte injections

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB453

   Plasmid

   #74835

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209664

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    hSyn

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB510

    Plasmid

    #74852

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

    Plasmid

    #229791

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. FU-tet-o-hKLF4

    Plasmid

    #19777

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    Klf4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121184

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    CMV-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    OsTIR1 codon optimized for human cells

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Venus-rab7a

    Plasmid

    #234743

    Purpose

    Mammalian expression of late endosome marker Venus-rab7a

    Depositor

    Nevin Lambert

    Article

    Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

    Insert

    Venus-rab7a (Rab7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

    Plasmid

    #110558

    Purpose

    miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    lacIq-Ptac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACE-GIRK4-mC-S

    Plasmid

    #172427

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    mCherry Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Use

    Tags

    Expression

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid

    #115775

    Purpose

    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    

    Use

    Tags

    EGFP; dsReD

    Expression

    Mammalian

    Mutation

    gp140 uncleaved (1190-91aa, KR > TG; truncated…

    Promoter

    HIV-1 (LTR)

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2_Firefly_degron-60A

    Plasmid

    #200396

    Purpose

    A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

    Depositor

    Xiao Wang

    Article

    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

    Insert

    Firefly luciferase - degron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

    Plasmid

    #114370

    Purpose

    expresses Flpo recombinase-dependent GtACR1

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR1-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MMLV-CAG-TVA-IRES-Puro

    Plasmid

    #172366

    Purpose

    Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSL1749 (pDonor, R*MmeI)

    Plasmid

    #160733

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJOG1368

    Plasmid

    #167301

    Purpose

    Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    FCY-UPP fusion gene
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. raACR-mCerulean3-pAAV2-hSyn

    Plasmid

    #204966

    Purpose

    Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    raACR
    

    Use

    AAV

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

    Plasmid

    #85069

    Purpose

    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    PH-PLCD1 (PLCD1 Human)
    

    Use

    Tags

    IRES-sYFP2-PH and mScarlet

    Expression

    Mammalian

    Mutation

    PLCD aa 1–170

    Promoter

    CMV

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-DiLACCO1

    Plasmid

    #218824

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate control biosensor

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    DiLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge