176,417 results

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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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EF.hICN1.CMV.GFP

Plasmid

#17623

Purpose

Depositor

Linzhao Cheng

Publication

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

ICN1 (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCI flag PCAF

Plasmid

#8941

Depositor

Yoshihiro Nakatani

Publication

Yang et al Nature. 1996 Jul 25. 382(6589):319-24.

Insert

PCAF (KAT2B Human)

Tags

flag

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Publication

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

72 Articles

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pKDsgRNA-p15

Plasmid

#62656

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Depositor

Kristala Prather

Publication

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Expression

Bacterial

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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FUW-tetO-hMYC

Plasmid

#20723

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

c-MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Publication

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pCDH-puro-cMyc

Plasmid

#46970

Purpose

lentiviral expression of c-Myc

Depositor

Jialiang Wang

Publication

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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pJFRC19-13XLexAop2-IVS-myr::GFP

Plasmid

#26224

Depositor

Gerald Rubin

Publication

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

myr::GFP

Expression

Insect

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

55 Articles

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pcDNA3-myc-CUL3

Plasmid

#19893

Depositor

Yue Xiong

Publication

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 3 (CUL3 Human)

Tags

3xmyc

Expression

Mammalian

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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lenti sgRNA(MS2)_puro optimized backbone

Plasmid

#73797

Purpose

optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pEGFP-N1-FLAG

Plasmid

#60360

Purpose

Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

Depositor

Sébastien Britton, Patrick Calsou

Publication

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Type

Empty backbone

Tags

EGFP and FLAG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Publication

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

103 Articles

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pBabe puro HA PIK3CA E545K

Plasmid

#12525

Depositor

Jean Zhao

Publication

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA E545K (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

E545K. Tumor-derived allele.

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pLVET-tTR-KRAB

Plasmid

#11644

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Publication

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hEF-1alpha, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSicoR

Plasmid

#11579

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Publication

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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EJ2GFP-puro

Plasmid

#44025

Depositor

Jeremy Stark

Publication

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ2GFP egfp-based chromosomal break reporter

Expression

Mammalian

Mutation

insertion of I-SceI, 3 frame stop, and flanking m…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pET His6 Sumo TEV LIC cloning vector (1S)

Plasmid

#29659

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SUMO, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

Plasmid

#20297

Purpose

Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

Depositor

Karl Deisseroth

Has service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

channelrhodopsin-2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

humanized ChR2 gene with histidine 134 changed to…

Promoter

EF1alpha

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

182 Articles

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