We narrowed to 7,452 results for: Lif;
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pLVX-CALPAIN-7-mCherry
Plasmid#180652PurposeLentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.DepositorInsertCALPAIN-7
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GCACCCAUACCUUUACAUUPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai
Plasmid#202571PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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PresentER-MSIIFFLPL (GFP)
Plasmid#102946PurposePresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)DepositorInsertMSIIFFLPL (Serpinf1 Synthetic, Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry
Plasmid#177183Purposeplasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertsgRNA targeting the PEAR-GFP plasmid
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1A
Plasmid#162692PurposeExpresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-CaNAR2
Plasmid#64732PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNAR2
UseTagsCerulean3, N-terminal 27 amino acids from CYP450,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-SypGFP
Plasmid#172380PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + SypEGFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)
Plasmid#166609PurposeEncodes Cre-inactivated hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag
Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-FAPP(PHD)
Plasmid#221748PurposeStable expression of GFP-FAPP(PHD) in mammalian cellsDepositorInsertFAPP (PLEKHA3 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–101PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFA-DAB2
Plasmid#162690PurposeExpresses DabAB2 inorganic carbon transport complexDepositorInsertDabAB2 inorganic carbon transport complex
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS-HsProm1s1-StayGold
Plasmid#211333PurposeVector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)DepositorInsertProminin 1 (PROM1 Human)
UseAAVTagsStayGoldExpressionMutationPromoterEF1aAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT007-GCN4scFV-VB
Plasmid#163387Purpose2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN systemDepositorInsertantiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-CaNAR2
Plasmid#64730PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertLyn-CaNAR2
UseTagsCerulean3, N-terminal targeting sequence from Lyn…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-OPTN
Plasmid#175760PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTNDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(M)Cas9
Plasmid#127384PurposeExpresses Cas9 at medium levelDepositorInsertuORF(M)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-LahT150
Plasmid#166785PurposeCleavage of double glycine motif in peptides to remove the leader sequenceDepositorInsertLahT150
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-1
Plasmid#92156PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-HygroR-T2A-TetOn3G
Plasmid#179887PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsHygromycin resistanceExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFE-Reu-FI-prk
Plasmid#162696PurposeExpresses R. eutropha Form IC rubisco and S. elongatus PrkDepositorInsertR. eutropha CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pFE-Rs-FI-prk
Plasmid#162697PurposeExpresses R. spaeroides Form IC rubisco and S. elongatus PrkDepositorInsertR. spaeroides Form IC rubisco and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS7
Plasmid#111764PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-E192C
Plasmid#180233PurposeExpresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domains (residue…PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EF1a-Flp-DOG-NW
Plasmid#75469PurposeFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertFlp-DOG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH3449
Plasmid#179576PurposePacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsBFPExpressionWormMutationPromoterPlgc-55BAvailable sinceMarch 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUChW Parkin shRNA Mul1 shRNA
Plasmid#78523PurposeLentiviral vector encoding two shRNAs and mCherry reporterDepositorUseLentiviral and RNAiTagsExpressionMutationPromoterH1Available sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
UseTagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma8
Plasmid#165496PurposeGreen fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-2
Plasmid#92157PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-ReaChR-P2A-dTomato
Plasmid#114368Purposeexpresses a Cre-dependent red light-shifted channelrhodopsin variantDepositorInsertReaChR-P2A-dTomato
UseAAVTagsdTomatoExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB
Plasmid#162699PurposeExpresses pHnCB10 derived carboxysome operon and prkDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDLX-TVA-2A-N2cG
Plasmid#172364PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neuronsDepositorInsertTVA + B19-N2cG
UseAAVTagsExpressionMammalianMutationPromotermDLXAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rTH-tTA
Plasmid#133268PurposeAn AAV genome that expresses tTA from the rat TH promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterrTHAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1-Fc
Plasmid#164220PurposeExpresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporterDepositorInsertSpike S1 domain (S SARS-CoV)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-303
Plasmid#159678PurposeGateway destination vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-E2_Crimson
Plasmid#172377PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertE2_Crimson
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 EGFR-sgRNA
Plasmid#188633PurposeA human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFRDepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
Plasmid#125224PurposeExpresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cellsDepositorInsertHA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-mRuby3
Plasmid#179727PurposeLentiviral expression of SYP-mRuby3 for use as a synaptic vesicle markerDepositorInsertSYP (Syp Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
Plasmid#125235PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only
