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Addgene

We narrowed to 1,978 results for: ENDOG

Showing: 1701 - 1750 of 1978 results
  1. GTU-c

    Plasmid
    #81093
    Purpose
    Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. GTU-y

    Plasmid
    #81095
    Purpose
    Gene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Insert
    mCitrine
    Use
    Cre/Lox
    Tags
    mCitrine
    Expression
    Yeast
    Mutation
    Mutated mCitrine for monomeric isoform (A206K L22…
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. GTU-ir

    Plasmid
    #81097
    Purpose
    Gene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. GTL-c

    Plasmid
    #81098
    Purpose
    Gene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. GTT-c

    Plasmid
    #81108
    Purpose
    Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
    Depositor
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. miR-451 Trap

    Plasmid
    #61487
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Insert
    miR-451 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pAC-BETA

    Plasmid
    #53272
    Purpose
    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Insert
    crtE, crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV-S5E2-FingR-PSD95-mGL

    Plasmid
    #217533
    Purpose
    Genetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivo
    Depositor
    Insert
    ZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)
    Use
    AAV
    Tags
    mGreenLantern
    Expression
    Mammalian
    Mutation
    Promoter
    S5E2 enhancer
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pUF1-dCas9-GCN5

    Plasmid
    #122509
    Purpose
    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.
    Depositor
    Insert
    dCas9-GCN5
    Use
    CRISPR
    Tags
    3*FLAG
    Expression
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid
    #86843
    Purpose
    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
    Depositor
    Insert
    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    Use
    Tags
    FLAG-HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TERT promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

    Plasmid
    #183609
    Purpose
    Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.
    Depositor
    Inserts
    Neo
    mKate2-3xNLS
    Use
    Mouse Targeting
    Tags
    3xNLS
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Promoter
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. LLP617_αGCN4-mCherry

    Plasmid
    #211785
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection
    Depositor
    Insert
    SnoopCatcher-DNMT3A
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Expression
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. Th-tdTomato

    Plasmid
    #159460
    Purpose
    Targeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassette
    Depositor
    Insert
    P2A-tdTomato
    Use
    Cre/Lox
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TH
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pTriEX-Antenna-GDI Cdc42

    Plasmid
    #101681
    Purpose
    "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al
    Depositor
    Insert
    Cdc42
    Use
    Tags
    6xHis and FRET Antenna (mCerulean-cpVenus)
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. LLP779_αGCN4-VP64-CO

    Plasmid
    #211772
    Purpose
    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection
    Depositor
    Insert
    aGCN4-VP64
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect VP64 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  18. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Insert
    SnoopCatcher-KRAB
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. pONSY-mCherry

    Plasmid
    #111874
    Purpose
    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Insert
    mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. GTL-ir

    Plasmid
    #81102
    Purpose
    Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Mutation
    codon optimized second iRFP to avoid internal rec…
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1701 - 1750 of 1978 results