19,171 results

1. pTKEI-Tre-C04

   Plasmid

   #79754

   Purpose

   Single fluorescent protein biosensor for trehalose

   Depositor

   David Savage

   Article

   Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

   Insert

   Tre-C04
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   pTrc

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-TALEN-L

   Plasmid

   #59025

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-L
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-hAR

   Plasmid

   #89078

   Purpose

   mammalian expression of human androgen receptor in pCMV5

   Depositor

   Elizabeth Wilson

   Article

   Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

   Insert

   human androgen receptor (hAR) full-length (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-TALEN-R

   Plasmid

   #59026

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-R
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBA2219

   Plasmid

   #38152

   Depositor

   David Waugh

   Article

   Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

   Insert

   DsbC (dsbC E. coli)
   

   Use

   Tags

   N-terminal leader peptide (cloning artifact)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry- SEC61B

   Plasmid

   #121160

   Purpose

   Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCE-mp53DD

   Plasmid

   #41856

   Purpose

   Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   CAG

   Available since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-GFP

    Plasmid

    #37825

    Depositor

    Edward Boyden

    Has Service

    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTet-O-Ngn2-puro

    Plasmid

    #52047

    Purpose

    3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

    Depositor

    Marius Wernig

    Article

    Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-and puromycin resistant cassette

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTet

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-CMV mCherry-GFP-LC3B WT

    Plasmid

    #123230

    Purpose

    Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Mito-7

    Plasmid

    #55102

    Purpose

    Localization: Mitochondria, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. murine TCR OTI-2A.pMIG II

    Plasmid

    #52111

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    T2A (from Thosea asigna virus)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mRuby3-C1

    Plasmid

    #127808

    Purpose

    mRuby3 C1 Cloning vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Use

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1 pegRNA1 with trimmed attP

    Plasmid

    #222346

    Purpose

    pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW-Cas9-Blast

    Plasmid

    #83481

    Purpose

    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

    Depositor

    Mohan Babu

    Inserts

    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    Tight Tre and human phosphoglycerate kinase 1 pro…

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pYL156 (TRV RNA2)

    Plasmid

    #148969

    Purpose

    Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    Modified TRV RNA2
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    see comments section below

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEGFP-LC3

    Plasmid

    #21073

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. AAVS1 pegRNA2 with trimmed attP

    Plasmid

    #222347

    Purpose

    pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP-C1-ER alpha

    Plasmid

    #28230

    Purpose

    Mammalian expression of Estrogen Receptor alpha fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

    Insert

    Estrogen Receptor alpha (ESR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLQ8001 - pCMV-R8.91 (D64V)

    Plasmid

    #202687

    Purpose

    R8.91 plasmid with mutated integrase (D64V)

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    gag pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D64V

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD-Flp

    Plasmid

    #122969

    Purpose

    Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

    Depositor

    Lydia Freddolino

    Article

    Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

    Insert

    Flp recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. PiggyBac PlayBack

    Plasmid

    #203312

    Purpose

    To all for PiggyBac gene insertion into Genome

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge