Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

20+ requests Clear filter

Applied Filters

20+ requests Clear filter

19,779 results

Showing: 10861 - 10890 of 19779 results

  2. pOpen-TEV protease

    Plasmid
    #165509
    Purpose
    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    TEV protease
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165509-plasmid-map-sequence-id-338590

    Enlarge

  3. pET28a SnoopTag-MBP

    Plasmid
    #72323
    Purpose
    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-MBP
    Tags
    N-terminal His6 tag. MBP is fused at the C-termin…
    Expression
    Bacterial
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72323-plasmid-map-sequence-id-137256

    Enlarge

  6. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid
    #119554
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    119554-plasmid-map-sequence-id-238815

    Enlarge

  12. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available Since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

    Enlarge

  13. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111848-plasmid-map-sequence-id-213454

    Enlarge

  16. pDC-UAS-nBitbow1.0(miniW)

    Plasmid
    #138329
    Purpose
    Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.
    Depositor
    Dawen Cai
    Article
    Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3
    Inserts
    mAmetrine
    tdKatushka2
    mNeonGreen
    mTFP1
    mKusabira-Orange2
    Tags
    human H2B
    Expression
    Insect
    Promoter
    10xUAS
    Available Since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138329-plasmid-map-sequence-id-275089

    Enlarge

  20. Dronpa3-C1

    Plasmid
    #54655
    Purpose
    Localization: C1 Cloning Vector, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Dronpa3
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. APJ-Tango

    Plasmid
    #66224
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    APJ (APLNR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10861 - 10890 of 19779 results