12,933 results

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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U6-sgH11-eCas9-T2A-BlastR

Plasmid

#172492

Purpose

For CRISPR-assisted HDR, Hipp11 locus

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

, BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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Full-length WT talin1

Plasmid

#182992

Purpose

express FL WT talin1 in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin1 WT (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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aP2-luciferase

Plasmid

#8858

Depositor

Bruce Spiegelman

Article

Graves et al Mol Cell Biol. 1992 Mar . 12(3):1202-8.

Insert

aP2 enhancer / promoter (Fabp4 Mouse)

Use

Luciferase

Tags

luciferase reporter

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tension Sensor

Plasmid

#182717

Purpose

expresses DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al iScience. 2025 Jul 9;28(8):113081. doi:

Insert

desmoglein-3 (Dsg3 Mouse)

Expression

Mammalian

Mutation

TSmod inserted between G726 and G727

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk2a2

Plasmid

#19727

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
Anti-GFP [N86/8R]

Plasmid

#177572

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra1

Plasmid

#179474

Purpose

Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra1
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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HA-KIF5B*-strep

Plasmid

#120170

Purpose

Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMK465 (mAB-Hygro)

Plasmid

#214391

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BrpmpTag-Hygro

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21

Plasmid

#105519

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Flag-PGC1a-6His

Plasmid

#67637

Purpose

AAV vector expressing PGC1a gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

PGC1a (Ppargc1a Mouse)

Use

AAV

Tags

6XHis and Flag tag

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST47-Arl13b12

Plasmid

#40872

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Tags

GFP

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV_NFL(WT)-FLAG

Plasmid

#182654

Purpose

Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

wild type mouse neurofilament light chain (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Nrp2.1-Fc-His

Plasmid

#72098

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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CMV500 A-C/EBPβ

Plasmid

#33363

Depositor

Charles Vinson

Insert

Cebpb (Cebpb Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see comments)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
pEGFPC1-Spop

Plasmid

#128872

Purpose

For mammalian expression of mouse Spop

Depositor

Aimin Liu

Article

Cai et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Spop (Spop Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt

Plasmid

#39531

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (Akt1 Mouse)

Tags

6G linker and EGFP

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mNRDc-V5

Plasmid

#51297

Purpose

expression of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-Gq-P2A-dTomato

Plasmid

#135635

Purpose

AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

Gq-P2A-dTomato

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA_Lifeact-GFP_NLS-mCherry

Plasmid

#69058

Purpose

Dual Fluorescent Reporter for F-actin and Nuclei

Depositor

Olivier Pertz

Insert

LifeAct-GFP_IRES_NLS-mCherry

Tags

Lifeact-GFP, NLS-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 light chain

Plasmid

#198254

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-mAscl1

Plasmid

#233152

Purpose

Retroviral expression of Ascl1

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Ascl1 (Ascl1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-GAT3-HA-mCherry

Plasmid

#117722

Purpose

Expresses a GABA transporter GAT-3 (Slc6a11)

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Slc6a11 (Slc6a11 Mouse)

Use

AAV

Tags

HA and mCherry

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk1a1(APF)

Plasmid

#19730

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(1959)-Pro-…

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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mATF4

Plasmid

#24874

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mouse ATF4 (Atf4 Mouse)

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pMSCVhyg-3xT7-CA-ChREBP

Plasmid

#171029

Purpose

Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Insert

CA-ChREBP (Mlxipl Mouse)

Use

Retroviral

Tags

3xT7

Expression

Mammalian

Mutation

amino acids 240 - 864

Promoter

5' LTR

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSP72 GSK3beta

Plasmid

#60125

Purpose

for transcription of GSK3beta

Depositor

Andrew McMahon

Insert

Glycogen synthase kinase 3 beta (Gsk3b Mouse)

Use

Cloning

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb promoter

Plasmid

#20124

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-CA-mDia1

Plasmid

#45583

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Insert mDia1 contains mutations V161D and N165D i…

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

Plasmid

#99278

Purpose

Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mVenus-IRES-mCherry
Puro

Use

Lentiviral

Tags

H2B, IRES, PGK, mCherry, and mVenus

Promoter

EF1 alpha-human and PGK-mouse

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PKC eta DN

Plasmid

#21246

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Mutation

K384R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3 Unc5C-FLAG

Plasmid

#72196

Purpose

Expresses full-length Unc5c with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c (Unc5c Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1 SIRT1-FL

Plasmid

#105670

Purpose

Mammalian expression construct of mouse SIRT1 full length isoform

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-PPARgamma2

Plasmid

#78768

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPARG (Pparg Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
paavCAG-Jx-rev-post-mGRASP-2A-dTomato

Plasmid

#34913

Purpose

JxON-post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

postsynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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12,933 results