4,133 results

1. pUltra-Chili-Luc

   Plasmid

   #48688

   Purpose

   3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

   Depositor

   Malcolm Moore

   Article

   Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-EGFP-Cdc42(T17N)

   Plasmid

   #12601

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (T17N) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-EGFP-Rac1-T17N

   Plasmid

   #12982

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 DN (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TLR6-YFP

   Plasmid

   #13020

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 6 (TLR6 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti CMV/TO Hygro empty (w214-1)

   Plasmid

   #17484

   Purpose

   3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-TLR1-YFP

   Plasmid

   #13014

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 1 (TLR1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. APEX2 - Tubulin in pEGFP

   Plasmid

   #66171

   Purpose

   APEX2 fused to the N-terminus of alpha tubulin

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 - alpha tubulin
   

   Use

   Tags

   Flag epitope tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   APEX2 contains 4 mutations relative to wt soybean…

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rc/CMV cyclin E

   Plasmid

   #8963

   Depositor

   Bob Weinberg

   Article

   Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

   Insert

   cyclin E (CCNE1 Human)
   

   Use

   Tags

   GPI linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLHKII-pGFPN3

   Plasmid

   #21920

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is the full length human HKII cDNA sequence …

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-MT GLI2 FL

    Plasmid

    #17648

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Use

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. Slug_pGL2

    Plasmid

    #31695

    Depositor

    Paul Wade

    Article

    Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

    Insert

    Slug (SNAI2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2/5

    Plasmid

    #104964

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 5

    Depositor

    Melina Fan

    Insert

    Rep2/Cap5
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p5

    Available since

    Oct. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFR-GFP

    Plasmid

    #32751

    Purpose

    Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP

    Depositor

    Alexander Sorkin

    Article

    Carter et al J Biol Chem. 1998 Dec 25;273(52):35000-7

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-luciferase-P2A-Neo

    Plasmid

    #105621

    Purpose

    3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2/1

    Plasmid

    #112862

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Truncated P5

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. MSCV-XZ066-EGFRvIII

    Plasmid

    #20737

    Depositor

    Alonzo Ross

    Article

    Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

    Insert

    EGFRvIII (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    EGFR with a 267 amino acid deletion in the extrac…

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. Cre Reporter

    Plasmid

    #62732

    Purpose

    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    LoxP-DsRED-STOP-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    LoxP sites

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLV-hTERT-IRES-hygro

    Plasmid

    #85140

    Purpose

    Lentiviral expression of hTERT, used to create immortalized cell lines

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEcgRNA

    Plasmid

    #166581

    Purpose

    CRISPR-Cas9-assisted genome editing in Escherichia coli

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FUGW-PercevalHR

    Plasmid

    #49083

    Purpose

    Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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