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Showing: 2241 - 2280 of 158569 results

  3. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

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  10. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

    Plasmid
    #170255
    Purpose
    Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    SV40 Large T Antigen
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170255-plasmid-map-sequence-id-334417

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  15. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid
    #192453
    Purpose
    intronized ttLbCas12a for in planta expression
    Depositor
    Tom Schreiber
    Article
    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4
    Insert
    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    Expression
    Mutation
    temperature-tolerant mutation (D156R)
    Promoter
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192453-plasmid-map-sequence-id-379029

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  19. eeBxb1_IVT

    Plasmid
    #222342
    Purpose
    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template
    Depositor
    David Liu
    Article
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    Template for in vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7 (inactivated)
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222342-plasmid-map-sequence-id-440249

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  29. Cd2ap-GFP

    Plasmid
    #221528
    Purpose
    Fluorescently tagged Cd2ap-GFP
    Depositor
    Alessandro Ori
    Insert
    Cd2ap (CD2AP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221528-plasmid-map-sequence-id-439382

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  40. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6b
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207852-plasmid-map-sequence-id-408170

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Showing: 2241 - 2280 of 158569 results