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Showing: 3051 - 3100 of 159111 results

  1. AAV-PR rep/cap

    Plasmid
    #197565
    Purpose
    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery
    Depositor
    Casey Maguire
    Article
    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089
    Inserts
    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    Use
    AAV
    Tags
    Expression
    Mutation
    contains 7mer insert PRPPSTH between amino acids …
    Promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197565-plasmid-map-sequence-id-388046

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  6. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Article
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  11. MBP-Benzonase

    Plasmid
    #218192
    Purpose
    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001
    Depositor
    Cheryl Arrowsmith
    Insert
    nucA
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    218192-plasmid-map-sequence-id-431003

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  12. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50590-plasmid-map-sequence-id-210724

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  13. rAAV2/MG1.2

    Plasmid
    #184541
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-MG1.2 capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R, Q588T; insertion o…
    Promoter
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184541-plasmid-map-sequence-id-361941

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  19. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74224-plasmid-map-sequence-id-238509

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  27. lenti-sgRNA puro

    Plasmid
    #104990
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104990-plasmid-map-sequence-id-443800

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  30. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

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  34. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106438-plasmid-map-sequence-id-309504

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  35. pmEGFP-C1

    Plasmid
    #36412
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    36412-plasmid-map-sequence-id-438461

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  36. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Promoter
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  37. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  44. pX330-BbsI-PITCh

    Plasmid
    #127875
    Purpose
    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly
    Depositor
    Peter Kaiser
    Article
    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127875-plasmid-map-sequence-id-418291

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  45. Creb1

    Plasmid
    #154942
    Purpose
    Expresses Creb1 in mammalian cells
    Depositor
    Georgios Stathopoulos
    Insert
    Creb1 (Creb1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154942-plasmid-map-sequence-id-308575

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  47. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid
    #105553
    Purpose
    AAV expression of Cre from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105553-plasmid-map-sequence-id-202451

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Showing: 3051 - 3100 of 159111 results