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161,252 results

Showing: 3051 - 3100 of 161252 results

  1. mCherry-Rab5a-7

    Plasmid
    #55126
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab5a
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55126-plasmid-map-sequence-id-133168

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  4. hsKIF5B(1-560)-HaloTag-FRB

    Plasmid
    #213899
    Purpose
    Imaging of KIF5B fused to HaloTag
    Depositor
    Lukas Kapitein
    Insert
    hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213899-plasmid-map-sequence-id-426209

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  9. TFORF3104

    Plasmid
    #144580
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NKX2-5 (NKX2-5 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144580-plasmid-map-sequence-id-404280

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  10. TFORF0005

    Plasmid
    #142525
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HIF3A (HIF3A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142525-plasmid-map-sequence-id-395956

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  12. TFORF0019

    Plasmid
    #141478
    Purpose
    Lentiviral vector for overexpressing the IRX4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IRX4 (IRX4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141478-plasmid-map-sequence-id-276570

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  15. pC-G418-YR

    Plasmid
    #61767
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61767-plasmid-map-sequence-id-367929

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  18. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

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  19. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102886-plasmid-map-sequence-id-194712

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  22. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid
    #206042
    Purpose
    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.
    Depositor
    Alisa Piekny
    Article
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Insert
    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    Use
    CRISPR and Synthetic Biology; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206042-plasmid-map-sequence-id-411117

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  24. pSIJ8

    Plasmid
    #68122
    Purpose
    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression
    Depositor
    Alex Nielsen
    Article
    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103
    Inserts
    flp recombinase
    rhaS
    rhaR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rhamnose
    Available since
    Dec. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68122-plasmid-map-sequence-id-243302

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  26. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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  27. B-GRAM-H

    Plasmid
    #211706
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Leucine
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211706-plasmid-map-sequence-id-419220

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  30. pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC

    Plasmid
    #221365
    Purpose
    Mammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Rho IPI-[1D4] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221365-plasmid-map-sequence-id-436834

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  31. pTipi2.1_Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa

    Plasmid
    #221366
    Purpose
    Mammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221366-plasmid-map-sequence-id-436839

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  34. pTXB1-pA-M.EcoGII

    Plasmid
    #192873
    Purpose
    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578
    Depositor
    Chong Tang
    Article
    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:
    Insert
    pA-M.EcoGII
    Use
    Tags
    3X Flag Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    192873-plasmid-map-sequence-id-380474

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  36. bNS2B47NS3

    Plasmid
    #155320
    Purpose
    co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155320-plasmid-map-sequence-id-318477

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  37. TFORF1062

    Plasmid
    #143751
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFATC1 (NFATC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143751-plasmid-map-sequence-id-407851

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  40. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  43. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Promoter
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  45. pMXs-Stat3-C

    Plasmid
    #13373
    Purpose
    Retroviral expression of dominant active mouse Stat3
    Depositor
    Shinya Yamanaka
    Article
    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.
    Insert
    signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Alanine 661 to cysteine and asparagine 66…
    Promoter
    Available since
    Jan. 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13373-plasmid-map-sequence-id-163452

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  46. pCF806-shABC2

    Plasmid
    #186714
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    186714-plasmid-map-sequence-id-382420

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  49. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

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Showing: 3051 - 3100 of 161252 results