166,201 results
-
Plasmid#102563PurposePiggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromoterN/AAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only
-
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCMVAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3
Plasmid#160250PurposeExpress FLAG-METTL3 in human cellsDepositorAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
Tags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-epegRNA-SV40-puro
Plasmid#240539PurposeLentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.DepositorInsertepegRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-3xFLAG-gateway-EGFP
Plasmid#122845PurposeN-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_blasti
Plasmid#194067Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-1
Plasmid#16400PurposeAdenoviral vector for use in AdEasy System.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralExpressionMammalianAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pXG237
Plasmid#141281PurposeATF4 translational reporterDepositorInsertuORF1/2 of ATF4 followed by mApple
UseLentiviralTagsmApplePromoterCMVAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVMutationCas9 D10APromoterCbhAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CNR1-Tango
Plasmid#66254PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro
Plasmid#234070PurposeFor lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
scFv-HaloTag-iLID
Plasmid#227390PurposeAdhesion module, scFv binds SunTagDepositorInsertscFv-HaloTag-iLID
UseLentiviralAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT57
Plasmid#223429PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible Luciferase reporter
Plasmid#64127PurposePhotoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.DepositorInsertluciferase
UseLuciferaseExpressionMammalianPromoterTetOx13-CMVAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HsPeg10rc3
Plasmid#174859PurposeExpresses recoded human Peg10 in mammalian cellsDepositorAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-mCherry FP
Plasmid#52114Purposea bicistronic IRES-mCherry containing retroviral vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-mCherryExpressionMammalianAvailable SinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-hTERT-HA
Plasmid#1772DepositorAvailable SinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
RMCE halo-flag DNA-PKcs
Plasmid#233918PurposeLow copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.DepositorAvailable SinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianAvailable SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pTARA
Plasmid#31491DepositorInsertRNA polymerase
ExpressionBacterialMutationN823DAvailable SinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
TMEM106B-EGFP
Plasmid#210366PurposeExpresses TMEM106B fused with EGFP in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
phCMV-coGALVenv-C4070A
Plasmid#172217PurposeExpresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectorsDepositorInsertEnvelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
ExpressionMammalianMutationCarries C-terminus of amphotropic MLV strain 4070…PromoterCMV immediate early promoterAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UPEmS-tevo
Plasmid#228894PurposeGolden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Centrin2 (Nigg UK185)
Plasmid#41147DepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti NG-ABE8e_P2A_GFP + PuroR
Plasmid#242000PurposeLentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.DepositorInsertNG-ABE8e
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pH2B-Electra2
Plasmid#179479PurposeNuclear labeling with blue fluorescent protein Electra2 fused to H2BDepositorInsertH2B-Electra2
TagsElectra2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
RMCE K>R DNA-PKcs
Plasmid#233917PurposeLow copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.DepositorInserthuman DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
ExpressionMammalianMutationK3753RAvailable SinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-v2-TC
Plasmid#194326PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0731
Plasmid#142201PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Caspase1-flag
Plasmid#21142DepositorAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
Orai1-GCaMP6f
Plasmid#73564PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertOrai1-GCaMP6f (ORAI1 Synthetic, Human)
TagsGCaMP6fExpressionMammalianPromoterCMV Immediate EarlyAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBPnlsLexA::p65Uw
Plasmid#26230DepositorInsertnlsLexA::p65 (RELA Human)
ExpressionInsectAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF0389
Plasmid#141600PurposeLentiviral vector for overexpressing the SMARCA4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ePop
Plasmid#50953PurposeExpresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillationsDepositorInsertsE lysis protein
LuxI
LuxR
MutationEarly stop codon introduced in mutated luxR resul…Available SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Long P2A
Plasmid#213613PurposeDetect the long-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Long P2A
ExpressionMammalianAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only