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Showing: 2561 - 2600 of 157662 results

  2. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Article
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Insert
    human progesterone receptor-B (PGR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89130-plasmid-map-sequence-id-176077

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  3. xlox(GFP)hTERT

    Plasmid
    #69809
    Purpose
    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a GFP marker
    Depositor
    David Ott
    Insert
    hTERT Reverse Transcriptase (TERT )
    Use
    Retroviral
    Tags
    none
    Expression
    Mutation
    Promoter
    MLV promoter
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    69809-plasmid-map-sequence-id-127377

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  4. eIF2a 1

    Plasmid
    #21807
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21807-plasmid-map-sequence-id-351672

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  8. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119945
    Purpose
    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    119945-plasmid-map-sequence-id-237587

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  10. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126035-plasmid-map-sequence-id-244699

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  12. pET29b-H6_Streptavidin_sfGFP

    Plasmid
    #124296
    Purpose
    His6-tagged Streptavidin GFP fusion protein
    Depositor
    Mark Arbing
    Insert
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    Use
    Tags
    His6 and T7 tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124296-plasmid-map-sequence-id-240453

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  18. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162073-plasmid-map-sequence-id-325292

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  20. pAS088

    Plasmid
    #211502
    Purpose
    AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Type
    Empty backbone
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211502-plasmid-map-sequence-id-417793

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  27. pYTK095

    Plasmid
    #65202
    Purpose
    Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65202-plasmid-map-sequence-id-110255

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  29. pGEX6P1-N-His

    Plasmid
    #119755
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    10xHis and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119755-plasmid-map-sequence-id-233662

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  32. TFORF1932

    Plasmid
    #142292
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    JUN (JUN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142292-plasmid-map-sequence-id-395770

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  33. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217653-plasmid-map-sequence-id-441459

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  35. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid
    #135391
    Purpose
    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.
    Depositor
    Fan Wang
    Insert
    TeLC-P2A-EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    135391-plasmid-map-sequence-id-266854

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Showing: 2561 - 2600 of 157662 results