Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

179,521 results

Showing: 2561 - 2600 of 179521 results

  1. MSCV-Cre

    Plasmid
    #24064
    Purpose
    Retroviral coexpression of Cre and EGFP
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    MESV 5' LTR
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    24064-plasmid-map-sequence-id-322907

    Enlarge

  8. pCMV5 mouse src

    Plasmid
    #13663
    Depositor
    Joan Brugge, Peter Howley
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    src (Src Mouse)
    Tags
    Myr
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 4, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13663-plasmid-map-sequence-id-154541

    Enlarge

  10. pEGFP-2xFYVE

    Plasmid
    #140047
    Purpose
    Expresses GFP-tagged HRS 2xFYVE in mammalian cells
    Depositor
    Harald Stenmark
    Publication
    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tandem HRS FYVE domain (Hgs Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    two FYVE domains (amino acids 147-223), linked by…
    Promoter
    CMV
    Available since
    May 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140047-plasmid-map-sequence-id-296064

    Enlarge

  14. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid
    #100854
    Purpose
    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jRGECO1a
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100854-plasmid-map-sequence-id-466906

    Enlarge

  15. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Publication
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87854-plasmid-map-sequence-id-175127

    Enlarge

  18. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71667-plasmid-map-sequence-id-243369

    Enlarge

  23. pX330-Cetn1/1

    Plasmid
    #50718
    Purpose
    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Publication
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50718-plasmid-map-sequence-id-75953

    Enlarge

  40. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized hfCas13d
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190034-plasmid-map-sequence-id-371641

    Enlarge

Showing: 2561 - 2600 of 179521 results