163,682 results

1. pHR CD19-FcGamma CAR

   Plasmid

   #113014

   Purpose

   for production of lentivirus encoding the CD19 CAR-Pfcgamma

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P intracellular GFP (CD19 Mouse, Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1a-NICD1

   Plasmid

   #46047

   Depositor

   Xin Chen

   Article

   Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

   Insert

   Notch1 (C-term) (Notch1 Mouse)
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain initiating at amino acid 1753

   Promoter

   EF1a

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO/GFP-GIGYF2

   Plasmid

   #141189

   Purpose

   Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   GIGYF2 (GIGYF2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Flag-HA-BAP1

   Plasmid

   #22539

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-Con/Fon-GCaMP6F

   Plasmid

   #137122

   Purpose

   Intersectional viral expression of GCaMP6F in cells expressing both Cre AND Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV5 and AAV8

   Insert

   Con/Fon-GCaMP6F
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2465

   Plasmid

   #142946

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   IRF6 (IRF6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Promoter

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET283xFlagPP7CPHis

   Plasmid

   #28174

   Depositor

   Kathleen Collins

   Insert

   PP7 coat protein optimized
   

   Use

   Tags

   6xhistidine and triple FLAG peptide

   Expression

   Bacterial

   Mutation

   C68A,C73A

   Promoter

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRB1017

   Plasmid

   #59936

   Purpose

   empty vector for gRNA cloning

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myosin-IIA-GFP

    Plasmid

    #38297

    Depositor

    Matthew Krummel

    Article

    Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

    Insert

    Myosin II A (Myh9 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. IDG_CLCC1_OE_1

    Plasmid

    #161672

    Purpose

    Overexpresses CLCC1

    Depositor

    Mike McManus

    Insert

    CLCC1 (CLCC1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPGxRF3

    Plasmid

    #138384

    Purpose

    T2A-GAL4 knock-in cassette

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL4
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF3347

    Plasmid

    #144823

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PITX1 (PITX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EYFP-p65

    Plasmid

    #111192

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    RELA (RELA Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINJ

    Plasmid

    #26045

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    HisGST3C-lacZ
    

    Use

    Tags

    MAHHHHHH_GST_LEVLFQGT

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-HIF1alpha-pcDNA3

    Plasmid

    #18949

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACEC3GH (Hyg)

    Plasmid

    #110084

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCWU6-xylR-E-luciferase

    Plasmid

    #190721

    Purpose

    fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

    Depositor

    Chenggang Wu

    Article

    Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

    Insert

    xylR-E-luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-FLAG-hAR

    Plasmid

    #89080

    Purpose

    mammalian expression of FLAG-tagged human androgen receptor

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

    Insert

    hAR (AR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pBac{3XP3::EYFP,attP}

    Plasmid

    #86860

    Purpose

    Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    attP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDest-527

    Plasmid

    #11518

    Purpose

    Gateway destination vector for bacterial expression; His tag

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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22. AKT-translocation-mStrawberry reporter

    Plasmid

    #158685

    Purpose

    This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAB076i7

    Plasmid

    #79201

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. A2_uSFFV

    Plasmid

    #153415

    Purpose

    Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    A*02:01-P2A-human beta2 microglobulin (B2M Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMCL-HA-MAPKK1-WT

    Plasmid

    #40808

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pPGK-T7/2-CD44v3 (fl)

    Plasmid

    #137814

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v3 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and N280D in the V3 region

    Promoter

    PGK

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Punc-4c.TIR1

    Plasmid

    #194053

    Purpose

    TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    codon-optimized for expression in C. elegans; Two…

    Promoter

    unc-4c

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmVen-Treslin

    Plasmid

    #211405

    Purpose

    mammalian expression of human Treslin N-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    Treslin (C15orf42 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-p150

    Plasmid

    #51412

    Purpose

    expression of full length of chicken DCTN1 p150Glued in mammalian cells

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    DCTN1 (DCTN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3-P4:gs:nls:CIBN

    Plasmid

    #135986

    Purpose

    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    P4 coiled-coil fused to the CIBN domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-HA-Sox2-IP

    Plasmid

    #13459

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDL17

    Plasmid

    #106483

    Purpose

    pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Inserts

    gam
    bet
    exo
    lacI
    rhaR
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. IDG_CHRNA2_OE_1

    Plasmid

    #161648

    Purpose

    Overexpresses CHRNA2

    Depositor

    Mike McManus

    Insert

    CHRNA2 (CHRNA2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-myc-ERK2-L4A-MEK1_fusion

    Plasmid

    #39197

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L4A = L33A, L37A, L40A, L42A

    Promoter

    CMV

    Available Since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny, Cesar Cuevas-Velazquez

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEMHE-mEGFP-Map4

    Plasmid

    #105571

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    MAP4 (Map4 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF1054

    Plasmid

    #142720

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PLAGL1 (PLAGL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

    Plasmid

    #62914

    Purpose

    Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-p65AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge