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Plasmid#216403PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 70, 443 to P…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y4S/Y8S/Y16S
Plasmid#216405PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutationsDepositorInsertEfg1-ΔDBD-Y4S/Y8S/Y16S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 4, 8 and 16 to Serine, and delet…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y64S/Y66S/Y69S
Plasmid#216406PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutationsDepositorInsertEfg1-ΔDBD-Y64S/Y66S/Y69S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 64, 66 and 69 to Serine, and del…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y101S/Y104S/Y106S
Plasmid#216407PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutationsDepositorInsertEfg1-ΔDBD-Y101S/Y104S/Y106S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 101, 104 and 106 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y401S/Y404S/Y409S
Plasmid#216409PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutationsDepositorInsertEfg1-ΔDBD-Y401S/Y404S/Y409S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 401, 404 and 409 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y450S/Y452S/Y456S
Plasmid#216410PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutationsDepositorInsertEfg1-ΔDBD-Y450S/Y452S/Y456S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 450, 452 and 456 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y478S/Y482S/Y484S
Plasmid#216411PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutationsDepositorInsertEfg1-ΔDBD-Y478S/Y482S/Y484S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 478, 482 and 484 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
JAB3479
Plasmid#190417PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3278
Plasmid#190402PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3616
Plasmid#190426PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2601
Plasmid#190388PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2600
Plasmid#190387PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1a/hU6Available sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2599
Plasmid#190386PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV488
Plasmid#177696PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast speciesDepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterTDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15484_HF-ABE8e
Plasmid#174122PurposeExpresses ABE8e fused with SpCas9-HF1DepositorInsertHF-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJ4M_HRP-1_FL
Plasmid#139117Purposeexpress HRP-1 with a C-terminal maltose binding protein tagDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV
Plasmid#133366PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV-Luc
Plasmid#133367PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-Luc
Plasmid#133360PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
kank1a_pJet1.2
Plasmid#121978Purposefor generating kank1a riboprobes for WISH in zebrafishDepositorInsertkank1a (si:dkey-146l2.1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1323
Plasmid#111125PurposeExpresses non-targeting_01224 sgRNA in pCR1068DepositorInsertNon-targeting gRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE(211-597)
Plasmid#82462PurposeExpression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cellsDepositorInsertmCE(211-597) (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
FNpCDNA3
Plasmid#45346DepositorTypeEmpty backboneUseTagsFLAG and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
DCIP
Plasmid#193860PurposeEncodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertNLS-mCherry-GFP1-10
UseTagsSV40 NLSExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-BFP
Plasmid#215720PurposeCatalytically inactive dCasRx with 2A-BFPDepositorInsertdCasRx
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-EGFP-CNA35
Plasmid#61603Purposebacterial expression of N-terminal fusion protein of CNA35 with EGFPDepositorInsertEGFP and CNA35
UseTagsHis tagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB
Plasmid#99391PurposeGateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)DepositorTypeEmpty backboneUseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cas9)
Plasmid#174865PurposeExpresses mouse Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by MmPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-CNA35
Plasmid#61607Purposebacterial expression of N-terminal fusion protein of CNA35 with mCherryDepositorInsertmCherry and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB-TRE-CRISPRoff-EF1A-TetOn
Plasmid#203355PurposeDrug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backboneDepositorInsertCRISPRoff-TetOn
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC014 - LwCas13a-msfGFP
Plasmid#91902PurposeExpresses active LwCas13a for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPRTagsNLS and msfGFP-NLS-3xHAExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-nCas9-PBE
Plasmid#98160PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-nCas9-UGI-NLS
UseMaizeTagsExpressionPlantMutationPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Puro-Cas9 donor
Plasmid#58409PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locusDepositorInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only