We narrowed to 14,607 results for: pET

1. pet 3d double tentacle delete CP

   Plasmid

   #13301

   Depositor

   John Cooper

   Article

   Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

   Insert

   capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Capping protein alpha 1 (with 28 amino acids dele…

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET-21a(+) SurE D9A

   Plasmid

   #12686

   Depositor

   SeWon Suh

   Article

   Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

   Insert

   SurE
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Aspartate 9 to Alanine

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

3. HOXA9 (murine) no-tags pET

   Plasmid

   #8493

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HoxA9 (Hoxa9 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   makes protein by Western

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pET22b-EC12-129/138

   Plasmid

   #47926

   Purpose

   Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Use

   Tags

   6xHis and TEV Cleavage

   Expression

   Bacterial

   Mutation

   Mutations C9A, K129C, D138C

   Promoter

   T7

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-28b(+)-DR RecR(34-220)

   Plasmid

   #12618

   Depositor

   SeWon Suh

   Article

   Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

   Insert

   Deinococcus radiodurans RecR(34-220)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-33

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pet 3d alpha tentacle delete CP

   Plasmid

   #13299

   Depositor

   John Cooper

   Article

   Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

   Insert

   capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits (CAPZB Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pet 3d beta tentacle delete CP

   Plasmid

   #13300

   Depositor

   John Cooper

   Article

   Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

   Insert

   capping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CP alpha subunit is intact with a mutated beta C-…

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

8. Rv2002 M3/pET-28b(+)N-tag

   Plasmid

   #13057

   Depositor

   SeWon Suh

   Article

   Yang et al Proc Natl Acad Sci U S A. 2003 Jan 21. 1

   Insert

   3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-28a PDZD8-T527D

   Plasmid

   #227851

   Purpose

   To express in E. coli PDZD8-T527D

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

   Insert

   PDZD8 (PDZD8 Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   T527D

   Promoter

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-28a PDZD8-T527E

    Plasmid

    #227852

    Purpose

    To express in E. coli PDZD8-T527E

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    PDZD8 (PDZD8 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    T527E

    Promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET45 His-proSAAS (27 kDa)

    Plasmid

    #231637

    Purpose

    Bacterial expression of full-length mouse proSAAS chaperone protein

    Depositor

    Iris Lindberg

    Article

    Hoshino et al J Neurochem. 2014 Feb;128(3):419-30. doi

    Insert

    Pcsk1N (Pcsk1n Mouse)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Kathleen Burns, Liam Holt

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ec holA-pET(11a)(ACH-)

    Plasmid

    #237233

    Purpose

    over-express E. coli holA (Pol III delta subunit)

    Depositor

    Michael O'Donnell

    Insert

    holA (delta) (holA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ec holB-pET(11a)(ACH-)

    Plasmid

    #237234

    Purpose

    over-express E. coli holB (Pol III delta prime subunit)

    Depositor

    Michael O'Donnell

    Insert

    holB (delta prime) (holB E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET23a-His-TEV-SpyCatcher3

    Plasmid

    #235149

    Purpose

    Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDuet1_6xHis-TEV-mCherry-WIPI3

    Plasmid

    #223763

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    WIPI3 (WDR45B Human)
    

    Use

    Tags

    6xHis-TEV-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST

    Plasmid

    #223744

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETDuet1_6xHis-TEV-mCherry-WIPI2d

    Plasmid

    #223725

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    6xHis-TEV-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDuet1_NIX(1-182aa)-THROMBIN-GST (WT)

    Plasmid

    #223733

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Bacterial

    Mutation

    aa 1-182 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (WT)

    Plasmid

    #223734

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    FUNDC1 soluble part (FUNDC1 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Bacterial

    Mutation

    aa 1-50 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-His-SUMO-LS4

    Plasmid

    #228450

    Purpose

    LS4 protein expression vector for bacteria

    Depositor

    Keisuke Fukunaga

    Article

    Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    LS4
    

    Use

    Tags

    10xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-His-SUMO-LS12

    Plasmid

    #228451

    Purpose

    LS12 protein expression vector for bacteria

    Depositor

    Keisuke Fukunaga

    Article

    Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    LS12
    

    Use

    Tags

    10xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET/His-Rab1a(Q70L)

    Plasmid

    #232919

    Purpose

    E. coli Expression

    Depositor

    James Hurley

    Insert

    RAB1A (RAB1A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q70L

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-Impact-T4gp32(L301G)

    Plasmid

    #228209

    Purpose

    Expression in BL21(DE3) of single strand DNA binding protein L301G

    Depositor

    Stephen Benkovic

    Insert

    61
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated leucine 301 to glycine (L301G), improves …

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-Impact-T4gp61(I342G)

    Plasmid

    #228208

    Purpose

    Expression in BL21(DE3) of T4 primase I342G

    Depositor

    Stephen Benkovic

    Article

    Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

    Insert

    61 (61 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated isoleucine 342 to glycine (I342G), improv…

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-HLA-A0201-ST3

    Plasmid

    #223220

    Purpose

    Expression of human HLA A2 heavy chain fused to Spytag003 for expression in bacteria

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    HLA--A2 heavy chain (HLA-A Human)
    

    Use

    Tags

    Spytag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZH81 pET21b Rat1-His

    Plasmid

    #231650

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    Rat1 (RAT1 Budding Yeast)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET45 His-proSAAS (21 kDa)

    Plasmid

    #231636

    Purpose

    Bacterial expression of 21 kDa mouse proSAAS chaperone protein

    Depositor

    Iris Lindberg

    Article

    Hoshino et al J Neurochem. 2014 Feb;128(3):419-30. doi

    Insert

    Pcsk1N (Pcsk1n Mouse)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-22b(+) pCopA sfgfp

    Plasmid

    #226374

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    sfGFP
    

    Use

    Tags

    6x HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    pCopA

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET29_anti-SARS-CoV-2-NM1226

    Plasmid

    #209422

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1226
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pET29_anti-SARS-CoV-2-NM1230

    Plasmid

    #209423

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1230
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pET29_anti-MERS-Nanobody-VHH-55

    Plasmid

    #209417

    Purpose

    Express anti-MERS-Nanobody-VHH-55 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-55
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pET29_anti-MERS-Nanobody-VHH-83

    Plasmid

    #209418

    Purpose

    express anti-MERS-Nanobody-VHH-83 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pET29_anti-MERS-Nanobody-VHH-84

    Plasmid

    #209419

    Purpose

    Express anti-MERS-Nanobody-VHH-84 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pET29_anti-SARS-CoV-2-Nanobody-C5

    Plasmid

    #209420

    Purpose

    Express anti-SARS-CoV-2-Nanobody C5 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-C5
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pET29_anti-SARS-CoV-2-Nanobody-F2

    Plasmid

    #209421

    Purpose

    Express anti-SARS-CoV-2-Nanobody F2 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-F2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pET29_anti-MERS-Nanobody-VHH-1

    Plasmid

    #206890

    Purpose

    Express anti-MERS-Nanobody-VHH-1 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pET21-pelB-LaG94-1--LaG94-14

    Plasmid

    #228973

    Purpose

    For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-1--LaG94-14
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28b-SUMO-FLAG-TeKaiC

    Plasmid

    #229222

    Purpose

    For expression of T. elongatus KaiC protein

    Depositor

    Andy LiWang

    Insert

    kaiC (kaiC T. elongatus)
    

    Use

    Tags

    SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28b-SUMO-SeKaiB-FLAG

    Plasmid

    #229219

    Purpose

    For expression of S. elongatus KaiB protein

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB S. elongatus)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET-6xHis-GFP(-7)-neg_tag1

    Plasmid

    #219419

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Insert

    GFP(-7)-neg_tag1
    

    Use

    Tags

    DEEEDD and MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a-hfYFP-TEV-Bcx

    Plasmid

    #191195

    Purpose

    Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Bcx fusion
    

    Use

    Tags

    6xHis and Bacillus circulans xylanase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28a-hfYFP-TEV-mScarlet-i

    Plasmid

    #191196

    Purpose

    Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28a-hfYFP-TEV-SAV

    Plasmid

    #191197

    Purpose

    Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET28a-LSSmGFP-TEV-mScarlet-i

    Plasmid

    #191199

    Purpose

    Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET28a-LSSmGFP-TEV-SAV

    Plasmid

    #191200

    Purpose

    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET22b-T5-MfnG-TEV-His

    Plasmid

    #191114

    Purpose

    Expresses MfnG-TEV-His in E. coli

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET28-PKP3(305-797)

    Plasmid

    #202599

    Purpose

    Expresses ARM domain of plakophilin3

    Depositor

    Tina Izard

    Article

    Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

    Insert

    plakophilin3 (PKP3 Human)
    

    Use

    Tags

    His8 tag with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1 to 304

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

    Plasmid

    #187708

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET28a(+)-NEDD4L_W3-W4-HECT (492)

    Plasmid

    #187709

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge