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Plasmid#227333PurposeMMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-PLK4
Plasmid#227311PurposeDonor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)DepositorInsertPLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Puro-moxGFP
Plasmid#227275PurposeAAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-CEP192
Plasmid#227290PurposeDonor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)DepositorInsertCEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScalps-Puro-FLAG-HA-Regnase-3 D252N
Plasmid#222664PurposeLentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationmutated aspartic acid 252 to asparagine to abroga…PromoterSFFVAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-CC1-CC1
Plasmid#225458PurposeExpress EGFP-fusion protein of FXR1 isoform a (CC1-CC1)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsEGFPExpressionMammalianMutationCC2 replaced with a second copy of CC1PromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-CC2mut (V361P)
Plasmid#225456PurposeExpress mEGFP-fusion protein of FXR1 isoform a (V361P)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationV361PPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FMR1iso1-CC2mut (V427P)
Plasmid#225466PurposeExpress mEGFP-fusion protein of FMR1 isoform 1 (V427P)DepositorInsertFMR1 isoform 1 (FMR1 Human)
UseTagsmEGFPExpressionMammalianMutationV427PPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-CC2-CC2
Plasmid#225459PurposeExpress mEGFP-fusion protein of FXR1 isoform a (CC2-CC2)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationCC1 replaced with a second copy of CC2PromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-Tudor-R rich
Plasmid#225478PurposeExpress mEGFP-GAPDH-Tudor-R fusionDepositorInsertGAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSII-PGK-FLPo-IRES-Puro
Plasmid#205989PurposeExpresses Flp recombinase and puromycin resistanceDepositorInsertsUseTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 MMP2 g1
Plasmid#170824PurposePiggyBac Cas13d sgRNA plasmid for MMP2 knockdownDepositorInsertCas13d MMP2 gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 MMP2 g2
Plasmid#170825PurposePiggyBac Cas13d sgRNA plasmid for MMP2 knockdownDepositorInsertCas13d MMP2 gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1
Plasmid#170826PurposePiggyBac Cas13d sgRNA plasmid for ANXA2 knockdownDepositorInsertCas13d ANXA2 gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2
Plasmid#170827PurposePiggyBac Cas13d sgRNA plasmid for ANXA2 knockdownDepositorInsertCas13d ANXA2 gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1
Plasmid#170828PurposePiggyBac Cas13d sgRNA plasmid for CLCN3 knockdownDepositorInsertCas13d CLCN3 gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2
Plasmid#170829PurposePiggyBac Cas13d sgRNA plasmid for CLCN3 knockdownDepositorInsertCas13d CLCN3 gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1
Plasmid#170822PurposePiggyBac Cas13d sgRNA plasmid for PPP2CA knockdownDepositorInsertCas13d PPP2CA gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)
Plasmid#221552PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorInsertgRNA targeting human Rab7A (RAB7A Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-IRAK4-mScarlet-P2A-Puro
Plasmid#221237PurposeStable expression of mouse IRAK4-mScarlet after Lentiviral transductionDepositorInsertIRAK4 (Irak4 Mouse)
UseLentiviralTagsmScarletExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Xlone Puro SNAP-eGFP-BASU
Plasmid#218452Purposeexpresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integrationDepositorInsertSNAP-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-PuroR-SV40 polyA-Z
Plasmid#218908Purposegolden gate cloningDepositorInsertmPGK-PuroR-SV40 polyA
UseTagsExpressionMammalianMutation297T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro 2xStrep-HPC4-Gb5
Plasmid#218177PurposeUsed to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cellsDepositorInsertG beta subunit 5 (GNB5 Human)
UseLentiviralTags2x Strep tag and HPC4ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-ngRNA+14_EF1a-puroR
Plasmid#207359PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR +14 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-ngRNA+15_EF1a-puroR
Plasmid#207360PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR +15 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-PlatTET-wpA-pPuroTK
Plasmid#214125PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-TET
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP703-eDHFR(69K6)-cRaf
Plasmid#184717PurposeiRafDepositorInsertmiRFP703-eDHFR(69K6)-cRaf (RAF1 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-4493NES(Booster-PKA)
Plasmid#184711PurposePKA biosensorDepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Puro_TAL1-short
Plasmid#210641PurposeOverexpression of TAL1-shortDepositorInsertTAL1-short (TAL1 Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V1AUC-DelStem-CD20-Puro
Plasmid#209758PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationMutations to disrupt the uORFs and the stem-loop …PromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S351A
Plasmid#206913PurposeExpression of mOct4 S351A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS351APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 T87A
Plasmid#206921PurposeExpression of mOct4 T87A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationT87APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S100A
Plasmid#206920PurposeExpression of mOct4 S100A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS100APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S102A
Plasmid#206919PurposeExpression of mOct4 S102A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS102APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 T228A
Plasmid#206918PurposeExpression of mOct4 T228A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationT228APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S229A
Plasmid#206917PurposeExpression of mOct4 S229A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS229APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S281A
Plasmid#206916PurposeExpression of mOct4 S281A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS281APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S282A
Plasmid#206915PurposeExpression of mOct4 S282A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS282APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S347A
Plasmid#206914PurposeExpression of mOct4 S347A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS347APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only