8,677 results

1. pET His6 MBP TEV LIC cloning vector (2M-T)

   Plasmid

   #29708

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-jGCaMP7s

   Plasmid

   #104463

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7s
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3/UBC9

   Plasmid

   #20082

   Depositor

   Edward Yeh

   Article

   Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

   Plasmid

   #163697

   Purpose

   Expresses c-terminal half of TurboID on surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.2-Cx43-HA

   Plasmid

   #49851

   Purpose

   Encodes human connexin 43 with a C-terminal HA epitope fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains several wobble mutations to confer resis…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYLCRISPR/Cas9P35S-N

   Plasmid

   #66191

   Purpose

   expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   cauliflower mosaic virus 35S promoter (P35S)

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP1122

   Plasmid

   #104159

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3/Flag-METTL3

   Plasmid

   #53739

   Purpose

   For expression of METTL3 (methyltransferase-like 3) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. phage-cmv-cfp-24xpp7

   Plasmid

   #40652

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   CFP-24xPP7
   

   Use

   Lentiviral

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. -1748 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32726

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Reporter gene

    Tags

    Expression

    Mutation

    Promoter

    -1748CCND1 promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-MetGFP

    Plasmid

    #37560

    Depositor

    David Rimm

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL2680

    Plasmid

    #85581

    Purpose

    Replicating base vector for constructing cpf1/CRISPR editing plasmids

    Depositor

    Himadri Pakrasi

    Article

    Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVSV eGFP RABV-G

    Plasmid

    #31833

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RSV-Flag-Brd4

    Plasmid

    #86616

    Purpose

    Flag tagged mouse Brd4 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd4 (Brd4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-SpyTagMBP

    Plasmid

    #35050

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyTagMBP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Simple peptide tag added to the N-terminus of MBP…

    Promoter

    T7 promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-U6-sgRNA-EGFP

    Plasmid

    #107721

    Purpose

    For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST17-UbE2S

    Plasmid

    #15807

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2S (UBE2S Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ppyCAG_RNaseH1_WT

    Plasmid

    #111906

    Purpose

    Express RNASEH1 in mammalian cell.

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMSCV-F-del Casp9.IRES.GFP

    Plasmid

    #15567

    Depositor

    David Spencer

    Article

    Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

    Insert

    FKBP12(V36)-p30Caspase9 (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    FKBP12 and HA epitope

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-Sec61b-C1

    Plasmid

    #90994

    Purpose

    Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mCherry-Sec61b (SEC61B Synthetic, Human)
    

    Use

    Tags

    mCherry (red fluorescence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. ER-mScarletI

    Plasmid

    #137805

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti CMV Blast empty (w263-1)

    Plasmid

    #17486

    Purpose

    3rd gen lentiviral empty control vector, CMV promoter, Blast 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PGK1p-Csy4-pA (Construct 2)

    Plasmid

    #55196

    Purpose

    Expresses Csy4 in Mammalian cells for site-specific RNA processing

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    Csy4/Cas6
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEYFP-NR1a

    Plasmid

    #17928

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJL 89

    Plasmid

    #98558

    Purpose

    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Enh 35S

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP(C1)-PP1gamma

    Plasmid

    #44225

    Depositor

    Laura Trinkle-Mulcahy, Angus Lamond

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP-1G (PPP1CC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFOS WT-GL3

    Plasmid

    #11983

    Purpose

    Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    c-fos promoter (Fos Mouse)
    

    Use

    Tags

    Luc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti-PGK-Hygro-PIP-FUCCI

    Plasmid

    #138714

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSR prdm3 (PRDM16 shRNA)

    Plasmid

    #15505

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 shRNA (Prdm16 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

35. moxBFP

    Plasmid

    #68064

    Purpose

    C-terminal fusion to oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCherry-LaminA-C-18

    Plasmid

    #55068

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTRKH3-slpGFP

    Plasmid

    #27168

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PX552

    Plasmid

    #60958

    Purpose

    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Inserts

    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin and U6

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GLP1R-Tango

    Plasmid

    #66295

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GLP1R (GLP1R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ChromosomesMembranesandCytoskeleton

    Plasmid

    #62450

    Purpose

    MXS_chaining vector with 4 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::H2B-TagBFPx3-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::mCherryx3-linker-betaActin-bGHpA
    CMV::Citrinex3-linker-alphaTubulin-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge