We narrowed to 5,620 results for: pcas

1. pT3TS-spCas9 ABE-Umax-ex1

   Plasmid

   #222141

   Purpose

   Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCag SE (Self-excising) FlpO-2A-Cre EV

   Plasmid

   #130986

   Purpose

   episomal expression of FlpO and Cre recombinases, self excising

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   protamine intron added to FlpO to prevent bacteri…

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-Akt-FoxO3a-KTR-EGFP

   Plasmid

   #125131

   Purpose

   Fluorescent reporter for Akt activity

   Depositor

   Kazuhiro Aoki

   Article

   Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

   Insert

   FOXO2 (FOXO3 Human)
   

   Use

   Tags

   Enhanced green fluorescent protein (EGFP)

   Expression

   Mammalian

   Mutation

   FoxO3A 1-402 amino acid

   Promoter

   CAG promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pattB-LSL-AAVR-F2A-spCas9

   Plasmid

   #202459

   Purpose

   The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

   Depositor

   Jan Carette

   Article

   Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

   Insert

   AAVR (AU040320 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   mCherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3TS-spCas9-ABE-Umax-rest1

   Plasmid

   #222144

   Purpose

   Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGG>nls-hCas9-nls-GFP

   Plasmid

   #99141

   Purpose

   Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   Cas9-GFP
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG PSAM4 5HT3 LC IRES GFP

   Plasmid

   #123066

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G Q139L Y217F

   Promoter

   CAG

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-SpyTag-Flag-SRSF3-V5-mCherry

   Plasmid

   #235091

   Purpose

   SRSF3 mCherry fusion protein without MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-SpyTag-Flag-SRSF1-V5-mCherry

    Plasmid

    #235089

    Purpose

    SRSF1 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS SARS-CoV-2 D614G Spike

    Plasmid

    #185692

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 D614G Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 D614G Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutation: D614G

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pC0043-PspCas13b-gRNA non target

    Plasmid

    #223698

    Purpose

    gRNA control for dPspCas13b-FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for luciferase control
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG PSAM4 GlyR IRES EGFP

    Plasmid

    #119739

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CAG

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-SpyTag-Flag-Lbr-V5-mCherry

    Plasmid

    #235094

    Purpose

    Lbr mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-Lbr-V5-mCherry (LBR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKRG3-mU6-PUb-hSpCas9-pAc

    Plasmid

    #162163

    Purpose

    Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    T2A-pAc

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

    Plasmid

    #132405

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKRG3-mU6-PUb-3xFLAG-hSpCas9

    Plasmid

    #162161

    Purpose

    Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-FLAG-puro-PTPIP51(1-470)

    Plasmid

    #170536

    Purpose

    Expresses PTPIP51(1-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

20. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAV pCAG-FLEX-tdTomato-WPRE

    Plasmid

    #51503

    Purpose

    Can be used to generate AAV virus that will express tdTomato in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

    Plasmid

    #86157

    Purpose

    FLS2-GFP expresssion

    Depositor

    Silke Robatzek

    Article

    Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    3xmyc and GFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid

    #80768

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCAGGS SARS-CoV-2 BA.2.12.1 Spike

    Plasmid

    #186032

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.2.12.1 Spike

    Depositor

    Marceline Côté

    Article

    Gong et al Viruses. 2022 Sep 30;14(10):2178. doi: 1

    Insert

    SARS-CoV-2 BA.2.12.1 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the BA.2 mutations (T19I, LPPA24S, G142D…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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