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Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1
Plasmid#170822PurposePiggyBac Cas13d sgRNA plasmid for PPP2CA knockdownDepositorInsertCas13d PPP2CA gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)
Plasmid#221552PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorInsertgRNA targeting human Rab7A (RAB7A Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-IRAK4-mScarlet-P2A-Puro
Plasmid#221237PurposeStable expression of mouse IRAK4-mScarlet after Lentiviral transductionDepositorInsertIRAK4 (Irak4 Mouse)
UseLentiviralTagsmScarletExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Xlone Puro SNAP-eGFP-BASU
Plasmid#218452Purposeexpresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integrationDepositorInsertSNAP-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-PuroR-SV40 polyA-Z
Plasmid#218908Purposegolden gate cloningDepositorInsertmPGK-PuroR-SV40 polyA
UseTagsExpressionMammalianMutation297T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro 2xStrep-HPC4-Gb5
Plasmid#218177PurposeUsed to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cellsDepositorInsertG beta subunit 5 (GNB5 Human)
UseLentiviralTags2x Strep tag and HPC4ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-ngRNA+14_EF1a-puroR
Plasmid#207359PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR +14 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-ngRNA+15_EF1a-puroR
Plasmid#207360PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR +15 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-PlatTET-wpA-pPuroTK
Plasmid#214125PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-TET
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP703-eDHFR(69K6)-cRaf
Plasmid#184717PurposeiRafDepositorInsertmiRFP703-eDHFR(69K6)-cRaf (RAF1 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-4493NES(Booster-PKA)
Plasmid#184711PurposePKA biosensorDepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Puro_TAL1-short
Plasmid#210641PurposeOverexpression of TAL1-shortDepositorInsertTAL1-short (TAL1 Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V1AUC-DelStem-CD20-Puro
Plasmid#209758PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationMutations to disrupt the uORFs and the stem-loop …PromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S351A
Plasmid#206913PurposeExpression of mOct4 S351A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS351APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 T87A
Plasmid#206921PurposeExpression of mOct4 T87A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationT87APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S100A
Plasmid#206920PurposeExpression of mOct4 S100A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS100APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S102A
Plasmid#206919PurposeExpression of mOct4 S102A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS102APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 T228A
Plasmid#206918PurposeExpression of mOct4 T228A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationT228APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S229A
Plasmid#206917PurposeExpression of mOct4 S229A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS229APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S281A
Plasmid#206916PurposeExpression of mOct4 S281A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS281APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S282A
Plasmid#206915PurposeExpression of mOct4 S282A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS282APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S347A
Plasmid#206914PurposeExpression of mOct4 S347A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS347APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro
Plasmid#201677PurposeEBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassetteDepositorInsertMIR302-3g-EEA-2g-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-MIR302-7guides-PGK-Puro
Plasmid#201960PurposeEBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassetteDepositorInsertMIR302-7g-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 1
Plasmid#198489Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 1DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 2
Plasmid#198490Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 2DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCtrl_EF1a-Puro-T2A-BFP
Plasmid#195500PurposeLentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequenceDepositorInsertsgRNA
UseCRISPR and LentiviralTagsBFP, Puro, and T2AExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV puro PRDM14 ΔSET domain
Plasmid#193680PurposeConstitutive retroviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseRetroviralTagsExpressionMutationDeletion of SET domainPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hIL1RN gRNA_1-MS2-Puro
Plasmid#192677PurposeLentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcriptionDepositorInsertHuman IL1RN activating gRNA #1 (IL1RN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOSR1-EGFP-PGK-Puro
Plasmid#186170PurposeKnock-in gene targeting in marmoset cells at OSR1 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL752-hGH-polyA-PGK-puroR
Plasmid#191682PurposeDonor plasmid backbone PGK-puroRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-EGFP-PGK-Puro
Plasmid#186167PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-HA
Plasmid#162103PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-ProtC-HA
UseLentiviralTagsVSVg-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-FLAG
Plasmid#162106PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-HA-FLAG
UseLentiviralTagsVSVg-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-AU1
Plasmid#162116PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to ProtC-HA-AU1
UseLentiviralTagsProtC-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-AU1
Plasmid#162087PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-HA-AU1
UseLentiviralTagsVSVg-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-FLAG
Plasmid#162095PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to ProtC-HA-FLAG
UseLentiviralTagsProtC-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W
Plasmid#163175PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tagDepositorInsertWWTR1 (WWTR1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W
Plasmid#163174PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tagDepositorInsertWWTR1 (WWTR1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only