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19,331 results

Showing: 241 - 280 of 19331 results

  11. pJH136

    Plasmid
    #47554
    Purpose
    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10
    Insert
    URA3
    Use
    Pcr template
    Tags
    Expression
    Mutation
    Promoter
    Native URA3 promoter, -243 to -1.
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    47554-plasmid-map-sequence-id-67878

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  12. pBad-HPII

    Plasmid
    #105839
    Purpose
    expresses E. coli HPII catalase with N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105839-plasmid-map-sequence-id-205523

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  15. SSTR2-Tango

    Plasmid
    #66503
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    SSTR2 (SSTR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66503-plasmid-map-sequence-id-345433

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  20. pAL204

    Plasmid
    #22282
    Purpose
    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2010
    Availability
    Academic Institutions and Nonprofits only
    22282-plasmid-map-sequence-id-80604

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  22. pAL113

    Plasmid
    #22471
    Purpose
    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    PhyB NT (PHYB Mustard Weed, Mouse)
    Use
    Synthetic Biology
    Tags
    10aaLinker-mCFP-HrasCT
    Expression
    Mammalian
    Mutation
    Y276H
    Promoter
    CMV
    Available since
    Nov. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    22471-plasmid-map-sequence-id-10325

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  29. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

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  30. pCMV-PE6c

    Plasmid
    #207853
    Purpose
    Mammalian expression of PE6c prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6c
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207853-plasmid-map-sequence-id-423164

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  32. pAAV2/5

    Plasmid
    #104964
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 5
    Depositor
    Melina Fan
    Insert
    Rep2/Cap5
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p5
    Available since
    Oct. 2, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    104964-plasmid-map-sequence-id-364714

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  38. pAAV2/1

    Plasmid
    #112862
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap1
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112862-plasmid-map-sequence-id-421935

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  40. pSF3-ultraID

    Plasmid
    #172878
    Purpose
    expresses ultraID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Article
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    ultraID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172878-plasmid-map-sequence-id-340526

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Showing: 241 - 280 of 19331 results