8,789 results

1. HA GSK3 beta K85A pcDNA3

   Plasmid

   #14755

   Depositor

   Jim Woodgett

   Article

   Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

   Insert

   GSK3 beta K85A (GSK3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K85A. Kinase dead mutant.

   Promoter

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. FLAG-FOXO3a WT

   Plasmid

   #8360

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. miniTurbo-V5_pRS415

   Plasmid

   #107168

   Purpose

   expresses V5-tagged miniTurbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMDR1-1202

   Plasmid

   #37627

   Depositor

   Kathleen Scotto

   Article

   Hu et al J Biol Chem. 2000 Jan 28;275(4):2979-85.

   Insert

   MDR1 promoter (ABCB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK7-HA-S6K1-WT

   Plasmid

   #8984

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ223 (dAsCpf1-SRDX)

   Plasmid

   #86209

   Purpose

   Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   dAsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   NLS and SRDX repressor and NLS

   Expression

   Plant

   Mutation

   D908A

   Promoter

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRRL_U2AF1_WT_mCherry

   Plasmid

   #84017

   Purpose

   expression of U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiPGK Puro DEST PKAKTRClover

   Plasmid

   #59153

   Purpose

   Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   PKA (PRKACA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG dCas9-KRAB-2A-EGFP

   Plasmid

   #92396

   Purpose

   CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9_KRAB_2A_EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS6-SMASh-YFP

    Plasmid

    #68852

    Purpose

    Expresses SMASh tag fused to YFP at N terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    SMASh-YFP
    

    Use

    Tags

    SMASh tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Talin-CS

    Plasmid

    #83377

    Purpose

    Control FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted at C-terminal o…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Kaede-N1

    Plasmid

    #54726

    Purpose

    Localization: N1 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Kaede

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHSN6I01

    Plasmid

    #50587

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUAS-luc2

    Plasmid

    #24343

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Luciferase
    

    Use

    Tags

    5XGAL4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE-zeo largeTcDNA

    Plasmid

    #1779

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1his p66shc (corrected)

    Plasmid

    #32574

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mApple-C1

    Plasmid

    #54631

    Purpose

    Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pScarlessHD-sfGFP-DsRed

    Plasmid

    #80811

    Purpose

    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    sfGFP-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cherry-LacRep

    Plasmid

    #18985

    Depositor

    Mirek Dundr

    Article

    Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

    Insert

    LacI
    

    Use

    Tags

    AviTag, SV40 NLS, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmIL-6 FL

    Plasmid

    #61286

    Purpose

    drives luciferase from Wt mouse IL-6 promoter (full length)

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IL-6 full length promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-C3-Exo70

    Plasmid

    #53761

    Purpose

    pEGFP-C3 plasmid that expresses Exo70

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Exo70 (EXOC7 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Rabbit GFP Cav 2.2 pMT2

    Plasmid

    #58737

    Purpose

    expression of GFP tagged rabbit Cav2.2 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    Cav2.2 (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-C1(2)delta

    Plasmid

    #21216

    Depositor

    Tobias Meyer

    Article

    Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

    Insert

    GFP-N2-PKCdelta-C1 (Prkcd Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C1(2) domains of PKC delta (rat) (Lab plasmid WC…

    Promoter

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-C1 beta-catenin WT

    Plasmid

    #16071

    Depositor

    Erin Schuman

    Article

    Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

    Insert

    beta-catenin (CTNNB1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYLCRISPR/Cas9P35S-B

    Plasmid

    #66190

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-hnRNPU-V5

    Plasmid

    #35974

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Synapsin-cyto-iATPSnFR1.0

    Plasmid

    #102556

    Purpose

    expresses cytoplasmic ATP sensor in neurons

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-a1AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRK7-HA-S6K1-KR

    Plasmid

    #8985

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K100R, kinase-inactive

    Promoter

    Available Since

    Jan. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-NL1(-)

    Plasmid

    #15260

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 (Nlgn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET12a-PAPI

    Plasmid

    #19961

    Depositor

    Jonathan Tait

    Article

    Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

    Insert

    Annexin V (ANXA5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLB1080

    Plasmid

    #37826

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    ATP7B (ATP7B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid

    #52421

    Purpose

    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

    Depositor

    Bertrand Collet

    Insert

    Fusion NLS-EGFP-P2A-Cherry-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1/opto-b2AR-EYFP

    Plasmid

    #20948

    Purpose

    Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-b2AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBMN HAYFP-LOV24

    Plasmid

    #49570

    Purpose

    regulate protein stability with light

    Depositor

    Thomas Wandless

    Article

    Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

    Insert

    YFP-LOV24
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    delete the last 3 aa from AsLOV2, with I532A muta…

    Promoter

    LTR

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 Flag TSC2 (S939A/T1462A)

    Plasmid

    #14131

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A/T1462A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A/T1462A

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pRK7-HA-S6K1-F5A-E389-R3A

    Plasmid

    #8991

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)

    Promoter

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-NL1B

    Plasmid

    #15261

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 with insert B (Nlgn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge