4,146 results

1. pCMV-flag YAP2 5SA

   Plasmid

   #27371

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP2 (YAP1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Serines at positions 61, 109, 127, 128, 131, 163,…

   Promoter

   Available since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMKV057

   Plasmid

   #133312

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc+. WUS2, IPT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUbi10, Nos, 35S

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSET-6xTR-TUBE

   Plasmid

   #110313

   Purpose

   E. coli expression and purification of 6xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   6xTR-TUBE (UBQLN1 Human)
   

   Use

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-SynA

   Plasmid

   #174860

   Purpose

   Expresses mouse syncytin A in mammalian cells

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   syncytin A (Syna Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Clover-mRuby2

   Plasmid

   #49089

   Purpose

   Clover-mRuby2 tandem fusion for FRET calibration

   Depositor

   Kurt Beam

   Insert

   Clover-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV SOCS-3

   Plasmid

   #11486

   Depositor

   Ronald Kahn

   Article

   Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

   Insert

   SOCS-3 (Socs3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Tet1

   Plasmid

   #60938

   Purpose

   Expresses Tet1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-myc-GLUT4-mCherry

   Plasmid

   #64049

   Purpose

   3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   GLUT4 (Slc2a4 Mouse)
   

   Use

   Lentiviral

   Tags

   c-Myc and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-UBR5

    Plasmid

    #52050

    Purpose

    CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 (UBR5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas9-sgAAVS1-1

    Plasmid

    #129726

    Purpose

    Expressing SpCas9 and sgAAVS1-1

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-1
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GA-NES

    Plasmid

    #61018

    Purpose

    ddGFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IF-GFP-ATRX

    Plasmid

    #45444

    Purpose

    ATRX expression vector

    Depositor

    Michael Dyer

    Article

    Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

    Insert

    ATRX (ATRX Mouse, Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Bacterial and Mammalian

    Mutation

    isoform 2, missing codon E124

    Promoter

    PCMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpliceExpress

    Plasmid

    #32485

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MIGR1

    Plasmid

    #27490

    Depositor

    Warren Pear

    Article

    Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV-RFP

    Plasmid

    #26001

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-RFP (H2BC21 A. victoria, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His6-MBP-uTEV3

    Plasmid

    #135464

    Purpose

    Bacterial expression of uTEV3 (full-length) protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    His6-MBP-uTEV3
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability) , I138T/S153N…

    Promoter

    T5

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-nCas9n

    Plasmid

    #47929

    Purpose

    expression of an optimized Cas9 for genome-editing in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    nls-zcas9-nls
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-Flpe

    Plasmid

    #13787

    Purpose

    Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-Brainbow3.0

    Plasmid

    #45176

    Depositor

    Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Insert

    pCAG-Brainbow3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3 Flag MKK7B2Jnk2a2

    Plasmid

    #19727

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pCDNA3 Flag MKK7B2Jnk1a1(APF)

    Plasmid

    #19730

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif Thr(1959)-Pro-…

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-EGFP-Cdc42(wt)

    Plasmid

    #12599

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (WT) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-ABE7.10

    Plasmid

    #102919

    Purpose

    expresses ABE7.10 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.10
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NLS-mCherry-LEXY (pDN122)

    Plasmid

    #72655

    Purpose

    Blue light-inducible nuclear export of mCherry

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    NLS-mCherry-LEXY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge