163,584 results

1. P4-PE

   Plasmid

   #211375

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   P4-PE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmStayGold_mStayGold_I3

   Plasmid

   #239339

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

   Depositor

   Torsten Wittmann

   Article

   Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

   Insert

   I3-01
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available Since

   June 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpCas9(D10A)-T2A-EGFP

   Plasmid

   #221230

   Purpose

   Express nCas9 nickase (D10) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ApoE4

   Plasmid

   #197148

   Purpose

   Expresses ApoE4 in Mammalian cell

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   ApoE4 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXR001: EF1a-CasRx-2A-EGFP

   Plasmid

   #109049

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   CasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rtn4b-GFP

   Plasmid

   #186952

   Purpose

   Expresses Rtn4b connected with C-terminus AcGFP1

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   RTN4B (RTN4 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FlipCherry-T2A-GFP

   Plasmid

   #124436

   Purpose

   Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipCherry-T2A-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVpuro-CMV-N-3xFLAG

   Plasmid

   #123223

   Purpose

   N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLIK-Neo

   Plasmid

   #25735

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-S33Y Beta-catenin

    Plasmid

    #19286

    Purpose

    Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S33Y (presumptive GSK3β phosphorylation site)

    Promoter

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. phDLX2-N174

    Plasmid

    #60860

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX2 (DLX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BFP-Sec61 beta

    Plasmid

    #49154

    Purpose

    expression of Sec61 beta fused to BFP, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRC0940 (circRNA entry vector)

    Plasmid

    #189967

    Purpose

    Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMD2.G-VSV-G-mut

    Plasmid

    #196508

    Purpose

    VSV-G mutant (K47Q, R354A)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    VSV-G
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG241 FKBP-CFAST10

    Plasmid

    #130814

    Purpose

    Expresses FKBP-CFAST10 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FKBP-CFAST10
    

    Use

    Tags

    Myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHIV-EGFP.Flag-EZH2

    Plasmid

    #220243

    Purpose

    Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV6-Neo-ISG15

    Plasmid

    #80404

    Purpose

    overexpression of ISG15

    Depositor

    Isidoro Martinez

    Article

    Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-RLuc8

    Plasmid

    #87121

    Purpose

    Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

    Depositor

    Sanjiv Sam Gambhir

    Article

    Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

    Plasmid

    #221233

    Purpose

    Express sgRNA targeting HEK3 loci with nCas9(D10A)

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pSIRV-NF-kB-eCFP

    Plasmid

    #118094

    Purpose

    Flourescent reporter for NF-kB activity

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eCFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NF-kB responsive element

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMH-SFB

    Plasmid

    #99391

    Purpose

    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hEF1a

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLL3.7

    Plasmid

    #11795

    Purpose

    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

    Depositor

    Luk Parijs

    Article

    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-C1 EGFP-3XNLS

    Plasmid

    #58468

    Purpose

    Expresses a nuclear-targeted eGFP on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Insert

    3X NLS with 3X stop codons
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAX APLP1

    Plasmid

    #30141

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Like Protein 1 (APLP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only