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We narrowed to 8,814 results for: CAG

Showing: 3601 - 3650 of 8814 results

  3. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and TRE3G
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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  11. Beta1 Q280* K418* ectodomain

    Plasmid
    #221399
    Purpose
    Mammalian expression of human integrin beta1 Q280* K418* ectodomain
    Depositor
    Timothy Springer
    Article
    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.
    Insert
    integrin beta1 Q280* K418* ectodomain (ITGB1 Human)
    Use
    Tags
    AVI tag, HRV3C cleavage site, basic coil, HA tag,…
    Expression
    Mammalian
    Mutation
    codon optimized for human mature _1 residues Q1 t…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221399-plasmid-map-sequence-id-439050

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  15. Beta1 N391* N562* ectodomain

    Plasmid
    #221400
    Purpose
    Mammalian expression of human integrin beta1 N391* N562* ectodomain
    Depositor
    Timothy Springer
    Article
    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.
    Insert
    integrin beta1 N391* N562* ectodomain (ITGB1 Human)
    Use
    Tags
    AVI tag, HRV3C cleavage site, basic coil, HA tag,…
    Expression
    Mammalian
    Mutation
    codon optimized for human mature _1 residues Q1 t…
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    221400-plasmid-map-sequence-id-437551

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  16. AAVS1-Hb9-CD14

    Plasmid
    #204344
    Purpose
    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    human CD14 (CD14 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hb9 (Mnx1) 9kb promoter fragment
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    204344-plasmid-map-sequence-id-407654

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  18. pCAT079

    Plasmid
    #180509
    Purpose
    Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180509-plasmid-map-sequence-id-364076

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  21. jk96

    Plasmid
    #120177
    Purpose
    CAG:mcherry:P2A:HA_NLGN
    Depositor
    Anthony Zador
    Insert
    CAG:mCherry:P2A:HA_NLGN
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    120177-plasmid-map-sequence-id-227832

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  22. jk95

    Plasmid
    #120176
    Purpose
    CAG:gfp:P2A:mycNRNX
    Depositor
    Anthony Zador
    Insert
    CAG:gfp:P2A:myc_NRXN
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    120176-plasmid-map-sequence-id-227831

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  23. SGEP-shRLuc-802

    Plasmid
    #188668
    Purpose
    control shRNA
    Depositor
    Lewis Cantley
    Insert
    shRLuc
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188668-plasmid-map-sequence-id-375059

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  24. pAAV Syn Barcode15

    Plasmid
    #226189
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode15
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226189-plasmid-map-sequence-id-447880

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  25. pCR8/GW/TOPO GFP

    Plasmid
    #69798
    Purpose
    Gateway entry vector for GFP
    Depositor
    Gerard Cagney
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pAAV Syn Barcode20

    Plasmid
    #226192
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode20
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226192-plasmid-map-sequence-id-447885

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  27. pTHAP7.1.0-gDNA

    Plasmid
    #132439
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    THAP7 (THAP7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132439-plasmid-map-sequence-id-266230

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  28. pCR8/GW/TOPO PCGF1

    Plasmid
    #69770
    Purpose
    Gateway entry vector for PCGF1
    Depositor
    Gerard Cagney
    Insert
    PCGF1 (PCGF1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. SGEP-sh-Hs-PHGDH-67

    Plasmid
    #188671
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188671-plasmid-map-sequence-id-375112

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  30. mU6-sgSik1_1st-hU6-sgSik3_2nd

    Plasmid
    #177217
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik3_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177217-plasmid-map-sequence-id-350029

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  31. SGEP-sh-Hs-PHGDH-1883

    Plasmid
    #188669
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188669-plasmid-map-sequence-id-375055

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  32. SGEP-sh-Hs-PHGDH-1968

    Plasmid
    #188673
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188673-plasmid-map-sequence-id-375111

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  33. SGEP-sh-Hs-PHGDH-1967

    Plasmid
    #188672
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188672-plasmid-map-sequence-id-375054

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  34. pCR8/GW/TOPO PCGF2

    Plasmid
    #69791
    Purpose
    Gateway entry vector for PCGF2
    Depositor
    Gerard Cagney
    Insert
    PCGF2 (PCGF2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. pICSL4723_ WRKY28_1

    Plasmid
    #126887
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126887-plasmid-map-sequence-id-249685

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  37. pENTRY4_ WRKY28_1

    Plasmid
    #126899
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126899-plasmid-map-sequence-id-250041

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  38. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    177232-plasmid-map-sequence-id-363978

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  39. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid
    #185060
    Purpose
    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185060-plasmid-map-sequence-id-375730

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  40. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

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  41. pNR4A1.1.0-gDNA

    Plasmid
    #112391
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1
    Depositor
    Kevin White
    Insert
    NR4A1 (NR4A1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112391-plasmid-map-sequence-id-230237

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  42. pCR8/GW/TOPO BMI

    Plasmid
    #69796
    Purpose
    Gateway entry vector for BMI
    Depositor
    Gerard Cagney
    Insert
    BMI (BMI1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pHK002.2

    Plasmid
    #235461
    Purpose
    Message phagemid carrying sgRNA2 (prom. J23119, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235461-plasmid-map-sequence-id-468611

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  45. pHK302.2

    Plasmid
    #235467
    Purpose
    Message phagemid carrying sgRNA2 (prom. J23110, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235467-plasmid-map-sequence-id-468575

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  46. pSUC01

    Plasmid
    #177204
    Purpose
    chromogenic reporter for gene knockout in Streptomyces
    Depositor
    Wenjun Guan
    Article
    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1
    Insert
    SaindC
    Use
    Synthetic Biology
    Tags
    NONE
    Expression
    Bacterial
    Mutation
    NONE
    Promoter
    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    177204-plasmid-map-sequence-id-382362

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Showing: 3601 - 3650 of 8814 results