4,136 results

1. YAP1-V5 in pLX304

   Plasmid

   #42555

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLM-vexGFP-Oct4

   Plasmid

   #22240

   Purpose

   2nd generation lentiviral vector

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   vexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMV::SypHy A4

   Plasmid

   #24478

   Depositor

   Leon Lagnado

   Article

   Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

   Insert

   SypHy (Syp Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE-FLAG-KD LKB1

   Plasmid

   #8593

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K78I: lysine 78 mutated to isoleucine; kinase dead

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2 mt-GFP (BamHI minus)

   Plasmid

   #15681

   Depositor

   Michael Klymkowsky

   Type

   Empty backbone

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   6x Myc and GFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. G6PD/pRK5

   Plasmid

   #41521

   Depositor

   Xiaolu Yang

   Article

   Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

   Insert

   glucose-6-phosphate dehydrogenase (G6PD Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIRESneo-EGFP-alpha Tubulin

   Plasmid

   #12298

   Depositor

   Patricia Wadsworth

   Article

   Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

   Insert

   EGFP-alpha Tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Reduced Expression GFP beta actin

   Plasmid

   #31502

   Depositor

   Rick Horwitz, Tim Mitchison

   Article

   Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

   Insert

   beta actin (ACTB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   91-544 of enhancer region of CMV promoter deleted

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTagBFP2-TOMM20-N-10

   Plasmid

   #55328

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. AAV-Cre-GFP

    Plasmid

    #68544

    Purpose

    AAV expression of Cre-GFP fusion

    Depositor

    Eric Nestler

    Article

    Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Caspase1-flag

    Plasmid

    #21142

    Depositor

    Junying Yuan

    Article

    Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

    Insert

    caspase-1 (Casp1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TagRFP-T-EEA1

    Plasmid

    #42635

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLYS1-FLAG-MitoGFP-HA

    Plasmid

    #50057

    Purpose

    Expression of GFP targeted to mitochondria with Flag and HA tags

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    Mito-GFP
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLQ1661-sgMUC4-E3(F+E)

    Plasmid

    #51025

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    optimized sgRNA (MUC4 Human, Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBA439

    Plasmid

    #85967

    Purpose

    Perturb-seq vector backbone

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G-CaMP3

    Plasmid

    #22692

    Purpose

    a single-wavelength GCaMP2-based genetically encoded calcium indicator

    Depositor

    Loren Looger

    Article

    Tian et al Nat Methods. 2009 Dec;6(12):875-81.

    Insert

    G-CaMP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N363D mutation (increased fluorescence)

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCW57-MCS1-P2A-MCS2 (RFP)

    Plasmid

    #80923

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO-p53-shRNA-427

    Plasmid

    #25636

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-427 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

    Plasmid

    #44028

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P402A, P577A, N813A

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMSCV-Zeo

    Plasmid

    #75088

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-HA-14-3-3 beta

    Plasmid

    #13270

    Depositor

    Michael Yaffe

    Insert

    14-3-3 beta (YWHAB Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TetO-FUW-oct4

    Plasmid

    #20323

    Depositor

    Rudolf Jaenisch

    Article

    Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

    Insert

    oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCMV4-3 HA/IkB-alpha(SS32,36AA)

    Plasmid

    #24143

    Depositor

    Warner Greene

    Article

    Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

    Insert

    IkB alpha (NFKBIA Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    S32A S36A

    Promoter

    Available since

    March 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. c-myc promoter (Del 1)

    Plasmid

    #16601

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 1) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-1 (XmnI->PvuII)

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMVTNT PINK1 N-myc

    Plasmid

    #13313

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-myc3-CUL1

    Plasmid

    #19896

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 1 (CUL1 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pY004 (pcDNA3.1-hFnCpf1)

    Plasmid

    #69976

    Purpose

    Expresses humanized FnCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hFnCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHAGE PGK-GFP-IRES-LUC-W

    Plasmid

    #46793

    Purpose

    2nd generation lentiviral vector

    Depositor

    Darrell Kotton

    Article

    Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

    Inserts

    PGK
    eGFP (eGFP )
    IRES
    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. hSERT pcDNA3

    Plasmid

    #15483

    Depositor

    Randy Blakely

    Article

    Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

    Insert

    hSERT (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCGN-SRF

    Plasmid

    #11977

    Purpose

    For mammalian expression of human SRF with an HA tag

    Depositor

    Ron Prywes

    Article

    Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLOX-gfp-iresTK

    Plasmid

    #12243

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    GFP, HSV1-TK
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pQFlag-USP7 WT puroR

    Plasmid

    #46751

    Purpose

    Retroviral vector that expresses wild type Flag-tagged human USP7

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid

    #26966

    Purpose

    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    eNpHR 3.0
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    ER export at 3' end of gene. Trafficking sig…

    Promoter

    EF1alpha

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3 Tat HA (P#29)

    Plasmid

    #14654

    Depositor

    Matija Peterlin

    Article

    Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

    Insert

    Tat (tat HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge