We narrowed to 6,724 results for: tac

1. Empty Bridge Control Zfp462-Klf4SE

   Plasmid

   #127666

   Purpose

   Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   gRNAs 129, 135, 115, 117
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLKO.1-TRC.mKO2_shHRASLS.2

   Plasmid

   #110324

   Purpose

   TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   HRASLS (PLAAT1 Homo sapiens, Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO.1-TRC.mKO2_shHRASLS.1

   Plasmid

   #110323

   Purpose

   TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   HRASLS (PLAAT1 Homo sapiens, Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLgw NKX2-5-V5-EcoDam

   Plasmid

   #98597

   Purpose

   Mammalian DamID lentiviral vector for NKX2-5 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR8/GW/TOPO-ELK1Y158A

   Plasmid

   #98628

   Purpose

   Gateway entry TOPO TA cloning vector ELK1 Y158A

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 Y158A (Elk1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Y158A

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR8/GW/TOPO-ETS1

   Plasmid

   #98621

   Purpose

   Gateway entry TOPO TA cloning vector for ETS1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ETS1 (Ets1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AK7 gRNA (BRDN0001146118)

   Plasmid

   #77914

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human AK7

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   AK7 (AK7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MAPKAPK2 gRNA (BRDN0001148536)

   Plasmid

   #77207

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAPKAPK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAPKAPK2 (MAPKAPK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAPKAPK2 gRNA (BRDN0001146296)

   Plasmid

   #77208

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAPKAPK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAPKAPK2 (MAPKAPK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CKS2 gRNA (BRDN0001149123)

    Plasmid

    #77141

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CKS2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CKS2 (CKS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HIPK1 gRNA (BRDN0001144888)

    Plasmid

    #76995

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HIPK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    HIPK1 (HIPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EPHA10 gRNA (BRDN0001146043)

    Plasmid

    #76099

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EPHA10

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EPHA10 (EPHA10 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CDK11A gRNA (BRDN0001147032)

    Plasmid

    #75891

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK11A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK11A (CDK11A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DGKK gRNA (BRDN0001146124)

    Plasmid

    #75844

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKK (DGKK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HNF4A_P2-198

    Plasmid

    #31065

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -198 to -1 of P2 HNF4A promoter

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX/PD-L1 WT

    Plasmid

    #121466

    Purpose

    Expression of GST-human PD-L1 in E. Coli

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid

    #154072

    Purpose

    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Inserts

    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    

    Use

    Tags

    GST, His6, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid

    #196974

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GST and mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX4T2-Ste7-EE

    Plasmid

    #52681

    Purpose

    expression of constitutively active Ste7 kinase in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    Ste7 (STE7 Budding Yeast)
    

    Use

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    S359E/T363E constitutively active

    Promoter

    tac

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

20. GST-FUS 465-526

    Plasmid

    #44983

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (465-526aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 465-526

    Promoter

    tac

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

21. GST-FUS 266-526

    Plasmid

    #44981

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (266-526aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 266-526

    Promoter

    tac

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

22. GST-FUS 356-526

    Plasmid

    #44982

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (356-526aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 356-526

    Promoter

    tac

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pGEX/PD-L1 3SA

    Plasmid

    #121467

    Purpose

    Expression of GST-human PD-L1 in E. Coli

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S176A, T180A, S184A

    Promoter

    Tac

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GST-FUS ΔNLS 1-513

    Plasmid

    #44984

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS ΔNLS (1-513aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 1-513

    Promoter

    tac

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pGEX-5X-1-GST-Armadillo

    Plasmid

    #242225

    Purpose

    Expression of GST-tagged human β-catenin Armadillo repeats without IDRs (deletion of a.a. 2-133 and 672-781) in E. coli

    Depositor

    Courtney Hodges

    Article

    Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deleted N terminal and C terminal IDRs from β-cat…

    Promoter

    tac

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMAL-c4X-MBP-ARID1A-IDR2

    Plasmid

    #242216

    Purpose

    Expression of MBP-tagged human ARID1A IDR2 (a.a. 1170-1610) in E. coli

    Depositor

    Courtney Hodges

    Article

    Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

    Insert

    ARID1A (ARID1A Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    ARID1A IDR2 (a.a. 1170-1610)

    Promoter

    tac

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX6p1_RBFOX2_RRM

    Plasmid

    #224293

    Purpose

    GST-RBFOX2(RRM domain) purification from bacteria

    Depositor

    Chuan He

    Article

    Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

    Insert

    RNA binding fox-1 homolog 2 RNA-recognition motif (RBFOX2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 74-167

    Promoter

    tac promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)

    Plasmid

    #180815

    Purpose

    Expression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1int (AA194-288, Y223F, Y248F) (PDCD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    Tac

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLViN-E-cadherin-GFP

    Plasmid

    #229710

    Purpose

    Lentiviral expression of E-cadherin-GFPTG in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CDH1 E-CADHERIN Human (CDH1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LMPd Amt OXCT1

    Plasmid

    #209407

    Purpose

    Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Luda et al Immunity. 2023 Sep 12;56(9):2021-2035.e8

    Insert

    Oxct1 shRNA (Oxct1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti_EF1a_Flag_TP53_WT

    Plasmid

    #183112

    Purpose

    Expresses flag-tagged p53 in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT2/shCol1a1/GFP4_Seq1.3

    Plasmid

    #206136

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. U6-sgH11-eCas9-T2A-BlastR

    Plasmid

    #172492

    Purpose

    For CRISPR-assisted HDR, Hipp11 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti_EF1a_Flag_TP53_R248W

    Plasmid

    #183114

    Purpose

    Expresses flag-tagged p53 with R248W mutation in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 248 to tryptophan

    Promoter

    EF1a

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. DYRK1A gRNA (BRDN0001145031)

    Plasmid

    #76755

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYRK1A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #69138

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBrain-ch-TOGKDP-GFP-shch-TOG

    Plasmid

    #69113

    Purpose

    Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Silent mutations to confer shRNA resistance.

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmCherryC1 VAP-B KD/MD

    Plasmid

    #226410

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-B (VAPB Human)
    

    Use

    Tags

    HA, T7-Xpress tag, and mCherry

    Expression

    Mammalian

    Mutation

    K87D/M89D mutant, unable to bind FFAT motifs

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid

    #129415

    Purpose

    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    RAB18 HDR template (RAB18 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Circular 200,100 (mIDUA)

    Plasmid

    #170123

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Idua Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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42. pLX-N-CTCF-mAID-Halo-donor

    Plasmid

    #139747

    Purpose

    Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    CTCF CRISPR knock-in (Ctcf Mouse)
    

    Use

    CRISPR

    Tags

    mAID-HaloTag

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pCMV 3xFLAG-LC3B Q116P G120

    Plasmid

    #129289

    Purpose

    Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid

    #122085

    Purpose

    Express M.EcoGII fused to CENPC with destabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of CENPC

    Expression

    Bacterial and Mammalian

    Mutation

    L341F, R930L (see comments below)

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. AXL gRNA (BRDN0001146919)

    Plasmid

    #76702

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AXL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AXL (AXL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 61426-hU6-shmFmr1-EF1a-mCherry

    Plasmid

    #157859

    Purpose

    Expresses a shRNA targeting mouse Fmr1 gene

    Depositor

    Xinyu Zhao

    Article

    Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLgw EcoDam-V5-ELK1

    Plasmid

    #98604

    Purpose

    Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. INCTbiosyn-pEF-GFP(rc)Bxb1

    Plasmid

    #127511

    Purpose

    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. BRD2 gRNA (BRDN0001148924)

    Plasmid

    #77984

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRD2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    BRD2 (BRD2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. KSR1 gRNA (BRDN0001146869)

    Plasmid

    #77858

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KSR1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    KSR1 (KSR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge