4,146 results

1. pLentiCMV Puro DEST ERKKTRClover

   Plasmid

   #59150

   Purpose

   Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BIC-Gag-CAS9

   Plasmid

   #119942

   Purpose

   encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

   Depositor

   Théophile Ohlmann, Emiliano Ricci, Philippe Mangeot

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GAG FMLV fused to spCAS9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-EGFP-Tau AP

   Plasmid

   #46905

   Purpose

   expresses EGFP tagged Tau AP in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau AP (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 Connexin43-GFP-APEX2

   Plasmid

   #49385

   Purpose

   Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Connexin43 - EGFP - APEX2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCherry-Peroxisomes-2

   Plasmid

   #54520

   Purpose

   Localization: Peroxisomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   Peroximal Targeting Signal 1 (PTS1) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-Brainbow-2.1 R

   Plasmid

   #18723

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

   Plasmid

   #37120

   Purpose

   DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   TdTomato-EGFP
   

   Use

   AAV and Cre/Lox; Cre-switch

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hTLR4

   Plasmid

   #13086

   Depositor

   Ruslan Medzhitov

   Insert

   TLR4 (TLR4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

   Plasmid

   #63931

   Purpose

   Empty AAV-RAM plasmid w. multiple cloning site (MCS)

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRAM and TRE

   Available since

   Dec. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-SIRT1 H363Y

    Plasmid

    #1792

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H363Y, deacetylase domain mutation

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-6xGLY-3xFLAG-hphMX4

    Plasmid

    #20755

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PGK-H2BmCherry

    Plasmid

    #21217

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CaMKIIa-EGFP

    Plasmid

    #50469

    Purpose

    EGFP under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Casp3 C163A-myc

    Plasmid

    #11814

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 catalytic mutant (CASP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C163A

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-R-GECO1

    Plasmid

    #32444

    Purpose

    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-smURFP

    Plasmid

    #80349

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Insert

    smURFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-CFP

    Plasmid

    #11179

    Purpose

    Mammalian expression vector for expression of CFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    ECFP from Aequorea victoria
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7-VEE-OKS-iM

    Plasmid

    #58972

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter and IRES

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAMD1-2

    Plasmid

    #61564

    Purpose

    Standardized mini-Tn5 delivery plasmid, kanamycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBabe B-Raf V600E

    Plasmid

    #17544

    Depositor

    Channing Der

    Article

    Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

    Insert

    B-Raf (BRAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CAG-GFP

    Plasmid

    #16664

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT3-EF1aH c-Met

    Plasmid

    #86498

    Purpose

    Mammalian expression of human c-Met

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid

    #141184

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Has Service

    Cloning Grade DNA

    Insert

    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    

    Use

    Tags

    Signal peptide from influenza HA and Superfolder …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMXs-Oct3/4

    Plasmid

    #13366

    Purpose

    Retroviral expression of mouse Oct3/4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HDAC7 Flag

    Plasmid

    #13824

    Purpose

    Mammalian expression of human histone deacetylase 7 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC7 (HDAC7 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV/D374Y-hPCSK9

    Plasmid

    #58379

    Purpose

    Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Insert

    proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D374Y

    Promoter

    HCRApoE/hAAT

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge