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170,823 results

Showing: 3041 - 3080 of 170823 results

  2. pET-APOBEC1-YTH

    Plasmid
    #200158
    Purpose
    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTH
    Tags
    6xHis, HA, and Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available Since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200158-plasmid-map-sequence-id-396323

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  8. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

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  17. pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249079
    Purpose
    Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    NNG-Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249079-plasmid-map-sequence-id-496312

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  20. pDAC435

    Plasmid
    #195238
    Purpose
    Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195238-plasmid-map-sequence-id-382946

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  24. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  26. pLenti-EML4-ALK variant 3a G1202R

    Plasmid
    #183830
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    G1202R
    Promoter
    CMV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183830-plasmid-map-sequence-id-360489

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  31. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid
    #223509
    Purpose
    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    Expression
    Mammalian
    Promoter
    CMV, SP6 and U6
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223509-plasmid-map-sequence-id-448627

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  32. pGreenFire1_E2F1RE

    Plasmid
    #112248
    Purpose
    expression of GFP and Luciferase under the control of E2F1 responsive element
    Depositor
    Simone Di Giovanni
    Insert
    E2F1RE (responsive element)
    Use
    Lentiviral
    Tags
    copGFP and luciferase
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112248-plasmid-map-sequence-id-213659

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  33. pKM427

    Plasmid
    #210788
    Purpose
    L5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transfer
    Depositor
    Kenan Murphy
    Article
    Murphy et al Methods Mol Biol. 2021;2314:301-321. doi
    Insert
    Defective hygromycin resistant gene
    Expression
    Bacterial
    Mutation
    Changed hygromycin resistant gene W190 codon (TGG…
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210788-plasmid-map-sequence-id-418045

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  38. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  39. pAG CMV Empty Puro

    Plasmid
    #237881
    Purpose
    Empty mammalian expression plasmid for cloning untagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237881-plasmid-map-sequence-id-469912

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Showing: 3041 - 3080 of 170823 results