We narrowed to 14,607 results for: pET

1. pET151-GST-PP-RPN13 (19-132) K97D

   Plasmid

   #125824

   Purpose

   Expresses GST-RPN13 (19-132) K97D in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 97 to aspartate

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET151-GST-PP-RPN13 (19-132) R104A

   Plasmid

   #125826

   Purpose

   Expresses GST-RPN13 (19-132) R104A in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to alanine

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET151-GST-PP-RPN13 (19-132) R104D

   Plasmid

   #125827

   Purpose

   Expresses GST-RPN13 (19-132) R104D in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to aspartate

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET151-GST-PP-RPN13 (19-132) R104E

   Plasmid

   #125828

   Purpose

   Expresses GST-RPN13 (19-132) R104E in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to glutamate

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Pet28a His6-HSPB7 (zebrafish) C49S

   Plasmid

   #110071

   Purpose

   Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Cysteine 49 to Serine

   Promoter

   T7

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-FFSARG-ddFLN4-HIS-ybbr

   Plasmid

   #101240

   Purpose

   minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FFSARG peptide, ddFLN4 fingerprint
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET11a asyn Q79N (TAC-->TAT)

   Plasmid

   #105733

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Insert

   alpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q79N

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pet151D-TOPO-BROX 1-411; K141E

   Plasmid

   #89865

   Purpose

   Expresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   BROX (BROX Human)
   

   Use

   Tags

   His Tag, V5 tag

   Expression

   Bacterial

   Mutation

   mutated lysine 141 to glutamic acid

   Promoter

   T7

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pet151D-TOPO-BROX 1-411; L212D

   Plasmid

   #89864

   Purpose

   Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   BROX (BROX Human)
   

   Use

   Tags

   His Tag, V5 tag

   Expression

   Bacterial

   Mutation

   mutated leucine 212 to aspartic acid

   Promoter

   T7

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET151-GFP-PP-RPN2 (918-953)

    Plasmid

    #73743

    Purpose

    Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pET151-GFP-PP-RPN2 (932-953)

    Plasmid

    #73745

    Purpose

    Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 932-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pET151-GFP-PP-RPN2 (918-947)

    Plasmid

    #73747

    Purpose

    Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-947 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pET151-GFP-PP-RPN2 (797-953)

    Plasmid

    #74228

    Purpose

    [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pET151-Vps4(101-437) L151D

    Plasmid

    #87736

    Purpose

    Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Leu 151 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET151-hUCH37 (ISF1)(M148D/F149D)

    Plasmid

    #71388

    Purpose

    Expression of hUCH37 (M148D/F149D) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
    

    Use

    Tags

    HIS-TEV sequence

    Expression

    Bacterial

    Mutation

    changed methionine 148 to aspartate, phenylalanin…

    Promoter

    T7

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET151-hUCH37 (ISF1)(M148A/F149A)

    Plasmid

    #61933

    Purpose

    Expression of hUCH37 (M148A/F149A) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Methionine 148 and Phenylalanine 149 were mutated…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

    Plasmid

    #61941

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-117) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 117, stop codon introdu…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. 213-petDuet1-GBD-LUMP--cdc42

    Plasmid

    #65973

    Purpose

    Dual expression of GBD-LUMP and cdc42

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Inserts

    LUMP
    cell division cycle 42 (CDC42 Human)
    

    Use

    Tags

    GBD (GTPase binding domain from ACK1; residues 44…

    Expression

    Bacterial

    Mutation

    constitutively active Cdc42 (Q61L)

    Promoter

    T7

    Available since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-22HT-MSI1(7-192)Q30D

    Plasmid

    #60346

    Purpose

    Q30D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    Q30D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Dec. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pET-22HT-MSI1(7-192)R53E

    Plasmid

    #60352

    Purpose

    R53E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pET-22HT-MSI1(7-192)G64A

    Plasmid

    #60351

    Purpose

    G64A mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G64A; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pET-22HT-MSI1(7-192)H83L

    Plasmid

    #60350

    Purpose

    H83L mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83L; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET-22HT-MSI1(7-192)G35D

    Plasmid

    #60347

    Purpose

    G35D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pET-22HT-MSI1(7-192)G35E

    Plasmid

    #60348

    Purpose

    G35E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pET-22HT-MSI1(7-192)H83F

    Plasmid

    #60349

    Purpose

    H83F mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83F; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET28a-ybbR-HIS-Spec(x2)-DocI

    Plasmid

    #58713

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Spec(x2)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET15b/GgVcl 1-851 A50I mutation

    Plasmid

    #46174

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-851 A50I (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 A50I mutation (talin binding domain mut…

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b/GgVcl 1-258 A50I mutation

    Plasmid

    #46175

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-258 A50I (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-258 (VD1) A50I mutation; talin binding defic…

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28b(+)_S. cerevisiae Hbs1(162-611)

    Plasmid

    #39337

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pET-21a(+)-DR RecR(K23A/R27A)

    Plasmid

    #12619

    Depositor

    SeWon Suh

    Article

    Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

    Insert

    Deinococcus radiodurans RecR (recR Deinococcus radiodurans)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysine 23 to Alanine, and Arginine 27 to …

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p141 pETF-GST RanBP10 G308R

    Plasmid

    #11094

    Depositor

    Ramesh Shivdasani

    Insert

    RanBP10 (Ranbp10 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G308R

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Pet28a His6-HSPB7 (zebrafish) C117S

    Plasmid

    #110072

    Purpose

    Express C117S mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 117 to Serine

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

    Plasmid

    #117981

    Purpose

    SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B1
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

    Plasmid

    #117982

    Purpose

    SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B2
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

    Plasmid

    #117983

    Purpose

    SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B2
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET His10-Draper delta ITAM

    Plasmid

    #126671

    Purpose

    Expression of His10-Draper delta ITAM mutant for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmic ITAM residues mutated to Phe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cytoplasmic ITAM residues mutated to Phe

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Kv7.4 BD (522-645, C643A)/pET28HMT

    Plasmid

    #111073

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 BD (522-645, C643A)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198467

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. pET264-pUC 24xMS2V6 Loxp KANr Loxp

    Plasmid

    #104393

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET mCherry LIC cloning vector (u-mCherry)

    Plasmid

    #29769

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET28a-6xHis-MBP-TEV site-TadA8r

    Plasmid

    #208318

    Purpose

    Overxpression MBP-TadA8r

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET His6 LIC cloning vector (2Bc-T)

    Plasmid

    #37236

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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50. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid

    #73440

    Purpose

    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC-P3A2-vioD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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