4,146 results

1. shCD44-2 pRRL

   Plasmid

   #19123

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against CD44 (CD44 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. GLP1R-Tango

   Plasmid

   #66295

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GLP1R (GLP1R Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUGW-PK-hLC3

   Plasmid

   #61460

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-CMV-jGCaMP7s

   Plasmid

   #104463

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7s
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-(-30)GFP

   Plasmid

   #62936

   Purpose

   Expression of 6xHis-(-30)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ90 - Pmyo-2::mCherry::unc-54utr

   Plasmid

   #19327

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-2::mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCX-OKS-2A

   Plasmid

   #19771

   Purpose

   Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Science. 2008 Nov 7. 322(5903):949-53.

   Insert

   Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Three genes are connected with the foot-and-mouth…

   Promoter

   Available since

   Jan. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. PLJR965

    Plasmid

    #115163

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Tyrosine hydroxylase

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV/TO Puro DEST (670-1)

    Plasmid

    #17293

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL2-NOS2Promoter-Luciferase

    Plasmid

    #19296

    Depositor

    Charles Lowenstein

    Article

    Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

    Insert

    NOS2 Promoter (Nos2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Beta-arrestin2 GFP WT

    Plasmid

    #35411

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBbi-Neo

    Plasmid

    #60525

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-TREX1

    Plasmid

    #27219

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-HA-BAX

    Plasmid

    #16587

    Depositor

    Bert Vogelstein

    Article

    Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

    Insert

    BAX alpha (BAX Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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22. PyronicSF/pcDNA3.1(-)

    Plasmid

    #124812

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 5xGAL4-TATA-luciferase

    Plasmid

    #46756

    Purpose

    Luciferase reporter gene for factors with GAL4 DNA binding domains

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minimal TATA

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3/UBC9

    Plasmid

    #20082

    Depositor

    Edward Yeh

    Article

    Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBABE-puro HGF

    Plasmid

    #10901

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    HGF (HGF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG_GPHN.FingR-mKate2-IL2RGTC

    Plasmid

    #46297

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR-mKate2-IL2RGTC (GPHN Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti CMV/TO GFP-Zeo DEST (719-1)

    Plasmid

    #17431

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-Flag-IDH1-R132H

    Plasmid

    #62907

    Purpose

    Expresses R132 mutant IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted Arg132 (R132)

    Promoter

    Available since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge