We narrowed to 14,145 results for: TIM
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Plasmid#137830PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Tags3x FLAGExpressionMammalianMutationMutated Leucine 71 to Lysine in CTEVp fragmentPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CAG_oROS-Gr_LF(C199S)
Plasmid#216114PurposeExpresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.DepositorInsertoROS-Gr_LF(C199S)
ExpressionMammalianPromoterCAGAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
Plasmid#115516PurposePlasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSPromoterUASAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin1 isoform1
Plasmid#72594Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN1 (SESN1 Human)
TagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK-Drosha(UniProt)-FLAG
Plasmid#164690PurposeExpress Drosha-FLAG in mammalian cellsDepositorAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIN1 (gpdA-cTerm-mGL-ptrA)
Plasmid#188735PurposeExpresses mGreenlantern for C-terminal taggingDepositorInsertgdpA-mGreenlantern PtrA
UseFilamentous fungal expressionTagsmGreenlanternPromotergpdAAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIN2 (gpdA-nTerm-mGL-ptrA)
Plasmid#188736PurposeExpresses mGreenlantern for N-terminal taggingDepositorInsertgdpA-mGreenlantern PtrA
UseFilamentous fungal expressionTagsmGreenlanternAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EFS-MCS-WPRE
Plasmid#163362PurposeThe empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectorsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB1678 CMV-HIVNES-GS-Cas13bt1
Plasmid#176316PurposeCMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)DepositorInserthuman codon optimized Cas13bt1
UseCRISPRTagsHIV NESExpressionMammalianPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRTH
Plasmid#136922PurposeExpresses ABEmax-NRTH in mammalian cellsDepositorInsertABEmax-NRTH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2(L348F)-CreN
Plasmid#75368PurposeExpresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinaseDepositorInsertCRY2 (CRY2 Mustard Weed)
TagsCre recombinase, residues 19-104ExpressionMammalianMutationL348FPromoterCMVAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAluYa5-neo-TET
Plasmid#51283Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertAluYa5
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterupstream pol III enhancer sequence of the 7SL gen…Available SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMY-IRES-eGFP
Plasmid#163361PurposeRetroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP markerDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 p53 S15D
Plasmid#69005Purposemammalian expression of human p53 S15D mutantDepositorAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
TagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianPromoterCAGAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Acd82
Plasmid#174100PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
ExpressionBacterialMutationN311S, C313G, V366A, W382TAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-p54
Plasmid#35379DepositorAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRCH
Plasmid#136923PurposeExpresses ABEmax-NRCH in mammalian cellsDepositorInsertABEmax-NRCH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHT-6xR.ads-PxylA-DI
Plasmid#47386PurposeExpresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsads
dxs
idi
Tags6xR and HisExpressionBacterialPromoterPgrac and PxylAAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-TEV-Nrf2
Plasmid#62455PurposeHalo-His6 tagged Nrf2 for E.coli expressionDepositorAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Pmel-1_TCRB
Plasmid#175288PurposeExpresses Pmel-1 TCR beta chainDepositorInsertT cell receptor beta chain (Tcrb Mouse)
UseSuitable for ivtExpressionMammalianPromoterCMV and T7Available SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Pmel-1_TCRA
Plasmid#175287PurposeExpresses Pmel-1 TCRA alpha chainDepositorInsertT cell receptor alpha chain (Tcra Mouse)
UseSuitable for ivtExpressionMammalianPromoterCMV and T7Available SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-Blast
Plasmid#159741PurposeExpresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 p53 S15A
Plasmid#69004Purposemammalian expression of human p53 S15A mutant.DepositorAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dominant negative Hsp90 beta
Plasmid#22480DepositorAvailable SinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
Plasmid#155125PurposeTol2 vector containing Cre-inducible QFGal4-switchDepositorInsertQFGal4-switch
UseCre/Lox; Zebrafish expressionPromoterUbiAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
SDHC-pHLuorin-sems
Plasmid#107767Purposelabeling of mitochondrial succinate dehydrogenase subunit C, pH measurementsDepositorInsertSubunit C of SDH fused to pHluorin (SDHC Human)
TagspHluorinExpressionMammalianPromoterCMVAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDule-Acd82
Plasmid#174099PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
ExpressionBacterialMutationN311S, C313G, V366A, W382TAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV sMTase (eGFP)
Plasmid#89930PurposeExpresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.DepositorInsertdCas9-MC-IRES-MN
TagsFlag and HAExpressionMammalianMutationdeactivated Cas9PromoterCMVAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir144[3072 nt]~451-CMV2-mir1
Plasmid#164682PurposeExpress miR-144[3072 nt]~451 and miR-1-1 in mammalian cellsDepositorExpressionMammalianPromoterCMVAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
6691 Bicistronic_ires_puro
Plasmid#64335PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance geneDepositorTypeEmpty backboneUseRetroviralAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S
Plasmid#62458Purposeexpresses his-tagged C151S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
TagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 151 of Keap1 to serinePromoterCMVAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-NLS
Plasmid#103819PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-PuroR-HA
Plasmid#163352PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection markerDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
h-Plekhm1_EGFP
Plasmid#73836PurposeExpress human Plekhm1 in mammalian cellsDepositorAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2A
Plasmid#115330Purposeexpresses mCherry-CHMP2A in mammalian cellsDepositorAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable SinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF9-STAT2C
Plasmid#37544DepositorAvailable SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMY-mCherry-P2A-PuroR-HA
Plasmid#163342PurposeRetroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection markerDepositorInsertmCherry
UseRetroviralExpressionMammalianPromoterLTRAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK Tgfbr3-HA neo
Plasmid#58508PurposeLentiviral expression vector for an inducible HA-tagged mouse Tgfbr3DepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-TVAmCherry-2A-oG
Plasmid#102985PurposeAAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombinationDepositorInsertsTVA-mCherry fusion protein after CRE-mediated recombination
Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
UseAAV; Adeno associated viral vectorAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA12-pHR-SFFV-BFP-B23
Plasmid#199443PurposeExpression of BFP-B23 in mammalian cellsDepositorAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky
Plasmid#138983PurposeIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38DepositorAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-33-MC_hammer
Plasmid#138990PurposeIVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-32-riot_punisher
Plasmid#138988PurposeIVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-32-riot_punisher
Plasmid#138987PurposeIVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-31-murderous_crow
Plasmid#138986PurposeIVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-31-murderous_crow
Plasmid#138985PurposeIVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only