19,178 results

1. pGWB401

   Plasmid

   #74795

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-HyPer7

   Plasmid

   #141071

   Purpose

   Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+) Laccase2 MCS Exon Vector

   Plasmid

   #69893

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   Laccase2 (stw Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ782 - HygroR

   Plasmid

   #190933

   Purpose

   Hygromycin resistance co-injection marker

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Hygromycin resistance gene
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-0

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-TurboID-NES_pLX304

   Plasmid

   #202008

   Purpose

   expresses TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-HA-OTUD5

   Plasmid

   #22610

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   OTUD5 (OTUD5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. ND4.2-Left TALE-G1397-N-DddA11-mCherry

   Plasmid

   #179682

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S1330I A1341V N1342S E1370K T1380I

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV306hsp+Lux

   Plasmid

   #26159

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

   Plasmid

   #91718

   Purpose

   AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

   Insert

   mEGFP-P2A-paAIP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII 1.3

   Available since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV1093

    Plasmid

    #111428

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-NIR-GECO2

    Plasmid

    #159603

    Purpose

    AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

    Depositor

    Robert Campbell

    Article

    Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

    Insert

    NIR-GECO2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-Tol2

    Plasmid

    #31831

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Lac

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SV-ABAactDA

    Plasmid

    #38247

    Depositor

    Jerry Crabtree

    Article

    Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

    Inserts

    VP16/PYL1
    Gal4DBD/ABI*
    

    Use

    Tags

    Flag, Gal4DBD, HA, and VP16

    Expression

    Mammalian

    Mutation

    D143A

    Promoter

    SV40 (IRES) and SV40p

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUGW

    Plasmid

    #14883

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-GFP-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Talin-CS

    Plasmid

    #83377

    Purpose

    Control FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted at C-terminal o…

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRX-hSTING-EGFP

    Plasmid

    #214149

    Purpose

    Retroviral transduction of EGFP-tagged STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_DNAseI

    Plasmid

    #182408

    Purpose

    Bacterial expression of DNaseI

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEGFP-pBAD

    Plasmid

    #54622

    Purpose

    Localization: Protein Expression Vector , Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pIRES proHB-EGF WT

    Plasmid

    #11602

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

22. dsRed-cent2

    Plasmid

    #29523

    Depositor

    Joseph Gleeson

    Article

    Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

    Insert

    Cent2 (CETN2 Human)
    

    Use

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-HA-RAPGEF2

    Plasmid

    #110162

    Purpose

    Expression of human RAPGEF2 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

    Insert

    RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTH3

    Plasmid

    #84453

    Purpose

    shuttle plasmid for sarA P1-YFP expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    YFP venus
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PyronicSF-mRuby2/pBI-CMV1

    Plasmid

    #124830

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Inserts

    PyronicSF
    mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBABE-hygro-hTERT

    Plasmid

    #1773

    Purpose

    Retroviral expression of hTERT. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS-ldhGFP

    Plasmid

    #27170

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AnkyrinB-2XHA

    Plasmid

    #31057

    Depositor

    Vann Bennett

    Article

    Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

    Insert

    Ankyrin B (ANK2 Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGFP-Kif5c

    Plasmid

    #71853

    Purpose

    mammalian expression of GFP-Kif5c

    Depositor

    Michelle Peckham

    Article

    Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

    Insert

    Kif5c (Kif5c Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Kif5c starts at AA3

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pHN781

    Plasmid

    #178828

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A (AR Human)
    

    Use

    Tags

    His8, TEV

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge