-
Plasmid#65695PurposeExpresses Golgi-ONL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_RhoB
Plasmid#65697PurposeExpresses CNL-RhoB in mammalian cellsDepositorInsertRhoB (RHOB Human)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE97 mFZ8-CS2+
Plasmid#16800DepositorInsertmFZ8-CS2+ (Fzd8 Mouse)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_ITPKA
Plasmid#65688PurposeExpresses ITPKA-CNL in mammalian cellsDepositorInsertinositol triphosphate 3-kinase A
UseLuciferaseTagsCNLExpressionMammalianMutationcontains residues 9-52PromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_zyxin
Plasmid#65681PurposeExpresses Zyxin-YNL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iE_C204S_pQE30
Plasmid#48632PurposeBacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionBacterialMutationinsertion E147B, c204SPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_3xNLS
Plasmid#65673PurposeExpresses NLS-CNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOGG113
Plasmid#133124PurposepL0M-PU-NoP, promotorless region used as negative controlDepositorInsertNoP promotorless region from plasmid pIJJ11268
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_3xNLS
Plasmid#65672PurposeExpresses NLS-YNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-GST
Plasmid#39352DepositorUseYeast genomic targetingTagsGST and kanMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI-Neto1
Plasmid#87672PurposeIn situ probe for mouse Neto1DepositorInsertNeto1 (Neto1 Mouse)
UseTagsExpressionBacterialMutationpartial sequence, (1244 to 2278bp of NM_144946.4)PromoterT7/T3Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/hTID1
Plasmid#13745DepositorInserthTid1 siRNA (DNAJA3 Human)
UseRNAiTagsExpressionMutationInverted repeat of 21 bp with 6 nuc. spacer.PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_Mito
Plasmid#65669PurposeExpresses Mito-YNL in mammalian cellsDepositorInsertcytochrome c oxidase subunit VIII
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_zyxin
Plasmid#65683PurposeExpresses Zyxin-ONL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_TUBB5
Plasmid#65680PurposeExpresses beta-tubulin-ONL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIND-GLucZ
Plasmid#228704PurposeInducible expression of Gaussia luciferase and shRNAmir of interestDepositorInsertsGaussia luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIV299
Plasmid#204967PurposeAMA1 plasmid with Aspergillus optimized Mad7 and argB selection markerDepositorInsertsMad7
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_SV40-BFP
Plasmid#224861PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_EF1a-BFP
Plasmid#224862PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
LPUtopia-8
Plasmid#224781PurposeLanding Pad donor vector for AAVS1 site integration in human and VeroE6 cellsDepositorInsertspuromycin N-acetyltransferase
enhanced Green Fluorescence Protein
Red Fluorescence Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a- mCherry-FIVAR-HIS-ybbR
Plasmid#169138PurposeE. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertmCherry
UseTags6x Histag, FIVAR tag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG42-GFP
Plasmid#220202Purposemini-Tn7 GFP expression vector - pBG42 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG35-GFP
Plasmid#220201Purposemini-Tn7 GFP expression vector - pBG35 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-KaNLuc
Plasmid#208855PurposeExpresses FLAG epitope-tagged KaNLuc "Red" BRET reporter in mammalian cells.DepositorInsertKaNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-Lumiscarlet
Plasmid#208854PurposeExpresses FLAG epitope-tagged Lumiscarlet "Red" BRET reporter in mammalian cells.DepositorInsertLumiscarlet
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-dKeNLuc
Plasmid#208856PurposeExpresses FLAG epitope-tagged dKeNLuc "Red" BRET reporter in mammalian cells.DepositorInsertdKeNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNLuc
Plasmid#208838PurposeExpresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNL
Plasmid#208839PurposeExpresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.DepositorInsertCeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GeNL
Plasmid#208841PurposeExpresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.DepositorInsertGeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA1
Plasmid#215398PurposeCm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)DepositorInsertcspA1
UseTags6xHis TagExpressionBacterialMutationCodon-optimized for E. coli BL21(DE3)PromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-GFP
Plasmid#220199Purposemini-Tn7 GFP expression vector - AraC/pBAD promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterAraC/pBADAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-GFP
Plasmid#220200Purposemini-Tn7 GFP expression vector - XylS/pM promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterXylS/pMAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP
Plasmid#220075Purposeexpresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBPDepositorInsertOSBPL5 H478/9A (OSBPL5 Human)
UseTagsExpressionMammalianMutationH478/9A, deltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5deltaORDdeltaTMD-FKBP
Plasmid#220074Purposeexpresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.DepositorInsertOSBPL5deltaORDdeltaTMD (OSBPL5 Human)
UseTagsExpressionMammalianMutationdeltaORDdeltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4562
Plasmid#200780PurposePflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFPDepositorInsertGtAR2
UseTagsmCherryExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only