4,073 results

1. pQLinkH

   Plasmid

   #13667

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   His, TEV, and attB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNW55

   Plasmid

   #22988

   Depositor

   Detlef Weigel

   Article

   Warthmann et al PLoS One. 2008 . 3(3):e1829.

   Insert

   osa-MIR528
   

   Use

   RNAi; Plant gene inactivation

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR207 SARS-CoV-2 E

   Plasmid

   #141273

   Purpose

   Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   E (E SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

   Plasmid

   #21845

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-BLM

   Plasmid

   #80070

   Purpose

   Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

   Depositor

   Nathan Ellis

   Article

   Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

   Insert

   BLM (BLM Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   If correct ID, the cDNA is from HeLa and has char…

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Casp3 C163A-myc

   Plasmid

   #11814

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

   Insert

   Caspase 3 catalytic mutant (CASP3 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C163A

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-NICD

   Plasmid

   #61540

   Purpose

   Expresses NOTCH-ICD in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   NOTCH-ICD (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NOTCH intracellular domain (aa1761-2555); A1943S…

   Promoter

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET17_K560_GFP_His

   Plasmid

   #15219

   Depositor

   Ron Vale

   Article

   Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

   Insert

   Human neuronal kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-DN-hCUL5-FLAG

   Plasmid

   #15823

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005 . 399():287-309.

   Insert

   Cullin5 (CUL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N441

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV::SypHy A4

    Plasmid

    #24478

    Depositor

    Leon Lagnado

    Article

    Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

    Insert

    SypHy (Syp Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV5S-F30-2xdBroccoli

    Plasmid

    #66845

    Purpose

    Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Use

    Tags

    5S rRNA

    Expression

    Mammalian

    Mutation

    Promoter

    5S

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mApple-VE-Cadherin-N-10

    Plasmid

    #54959

    Purpose

    Localization: Tight Junctions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    VE-Cadherin (CDH5 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    I503T

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PKC alpha CAT

    Plasmid

    #21234

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-325

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 MEKK1 WT

    Plasmid

    #12181

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Arp3-pmCherryC1

    Plasmid

    #27682

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Arp3 (ACTR3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLH-nmsgRNA1.1

    Plasmid

    #64115

    Purpose

    Vector for expression of Nm sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LacZ in pLX304

    Plasmid

    #42560

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2xV5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET23b-Casp3-His

    Plasmid

    #11821

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-Jmjd3-F

    Plasmid

    #17440

    Purpose

    Expression of Jmjd3 in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    JMJD3 (Kdm6b Mouse)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.2/v5-DEST hGlut1

    Plasmid

    #18085

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut1 (SLC2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDusk

    Plasmid

    #43795

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA 3.1(-) mouse C/EBP beta (LAP)

    Plasmid

    #12557

    Depositor

    Peter Johnson

    Insert

    CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWPT-GFP

    Plasmid

    #12255

    Depositor

    Didier Trono

    Insert

    EGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mOrange2-N1

    Plasmid

    #54568

    Purpose

    Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Nathan Shaner, Roger Tsien, Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. tetO-GATA4

    Plasmid

    #46030

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    R43Q, G50S and N197S

    Promoter

    tetO

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs-Stat3-C

    Plasmid

    #13373

    Purpose

    Retroviral expression of dominant active mouse Stat3

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Alanine 661 to cysteine and asparagine 66…

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LeGO-iC2

    Plasmid

    #27345

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EdTP

    Plasmid

    #24311

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP-C1-Ataxin3Q28

    Plasmid

    #22122

    Depositor

    Henry Paulson

    Article

    Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

    Plasmid

    #141387

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. PGC-1 alpha promoter luciferase delta CRE

    Plasmid

    #8888

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Site-directed mutagenesis to remove the CREB bind…

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-AKAR4

    Plasmid

    #61619

    Purpose

    FRET-based biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4
    

    Use

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMW#3

    Plasmid

    #13350

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    LacZ reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. aMHC-mCherry-Rex-Blasticidin

    Plasmid

    #21228

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin2 (CUL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge