165,528 results

1. pJRH-1362 scFv entry plasmid

   Plasmid

   #201912

   Purpose

   Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV/SB11

   Plasmid

   #26552

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   Sleeping Beauty
   

   Expression

   Bacterial

   Mutation

   Methionine 243 to Glutamine Threonine 136 to Arg…

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. EcMscS

   Plasmid

   #78555

   Purpose

   E. coli Mechanosensitive Channel of Small Conductance

   Depositor

   Douglas Rees

   Article

   Bass et al Science. 2002 Nov 22;298(5598):1582-7.

   Insert

   Mechanosensitive Channel of Small Conductance
   

   Tags

   6x Histidine Tag

   Promoter

   T7 Promoter

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

   Plasmid

   #84251

   Purpose

   Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Bxb1

   Plasmid

   #182142

   Purpose

   Expression of human codon-optimized Bxb1

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   human codon optimized Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-U6-epegRNA-SV40-puro

   Plasmid

   #240539

   Purpose

   Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

   Depositor

   Wensheng Wei

   Article

   Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

   Insert

   epegRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_011

   Plasmid

   #59702

   Purpose

   This lentiviral vector can be used to assay Cas9 activity.

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-AP.Adamts2.1-GFP-WPRE

   Plasmid

   #211488

   Purpose

   expresses GFP under artificial promoter Adamts2

   Depositor

   Georg Keller

   Article

   O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

   Insert

   GFP
   

   Use

   AAV

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26-MBP-GLIC

    Plasmid

    #20887

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
    

    Tags

    His, MBP, and pelB

    Expression

    Bacterial

    Available Since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLM-CMV-R-Cre

    Plasmid

    #27546

    Purpose

    Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

    Insert

    mCherry_P2A_Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Promoter

    CMV

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-DLL4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156889

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    DLL4 (DLL4 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

    Plasmid

    #108770

    Purpose

    Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa397 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAC_N_FEV

    Plasmid

    #167757

    Purpose

    Expresses FEV in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    FEV (FEV Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-LAMP1-mCherry

    Plasmid

    #214274

    Purpose

    Mammalian expression of LAMP1-mCherry (lysosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    LAMP1-mCherry (Lamp1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CL20_mEGFP_TMPRSS2_ERG

    Plasmid

    #205943

    Purpose

    Express mEGFP-tagged fusion protein, TMPRSS2_ERG from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    TMPRSS2_ERG (TMPRSS2, ERG Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

    Plasmid

    #108833

    Purpose

    Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa28 to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS and GAI

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid

    #176027

    Purpose

    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    rtTA3
    Luc2
    mtagBFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. hSyn1 HRP-TM

    Plasmid

    #231500

    Purpose

    Expressing HRP on the cell surface of neurons

    Depositor

    Baljit Khakh

    Article

    Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

    Insert

    HRP
    

    Use

    AAV

    Tags

    Igk, HA, Myc, PDGFR-beta transmembrane domain

    Expression

    Mammalian

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pExp-His-zBasic-RBD

    Plasmid

    #194999

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli

    Depositor

    Marko Hyvönen

    Article

    Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

    Insert

    RBD (S SARS-CoV-2)
    

    Tags

    His8-Zbasic

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. RF-118-UB-BRCA1

    Plasmid

    #78960

    Purpose

    Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

    Depositor

    Richard Fahlman

    Article

    Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    3x Flag and ubiquitin

    Expression

    Mammalian

    Mutation

    delta 1-1155

    Promoter

    CMV

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLX401-INK4A

    Plasmid

    #121919

    Purpose

    Doxycycline-inducible expression of p16-INK4A product of CDKN2A

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    p16-INK4A (CDKN2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE Tet ON

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pNP3254 [pNP2922 - ISCro4 Enhanced DBL (DBL3, WT) + ISCro4 Recombianse (WT]

    Plasmid

    #247257

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA101-179 ins179(rc(101-111) (DBL3)
    

    Expression

    Mammalian

    Mutation

    ins179(rc(101-111)

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pNP3386 [For use in genome insertion and off-target assessment]

    Plasmid

    #247258

    Purpose

    Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pvPE-V2

    Plasmid

    #240286

    Purpose

    Encodes second generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

    Depositor

    Lusheng Huang

    Article

    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

    Insert

    pvPE-V2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pvPE-V3

    Plasmid

    #240287

    Purpose

    Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

    Depositor

    Lusheng Huang

    Article

    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

    Insert

    pvPE-V3
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NPY EGFP

    Plasmid

    #245034

    Purpose

    Secretory granule marker for endocrine cells

    Depositor

    Thomas Martin

    Insert

    NPY (NPY Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NanoLuc-BAZ2A Fusion Vector

    Plasmid

    #238659

    Purpose

    Express NanoLuc-BAZ2A Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    BAZ2A (BAZ2A Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. NanoLuc-EP300 Fusion Vector

    Plasmid

    #238648

    Purpose

    Express NanoLuc-EP300 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    EP300 (EP300 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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32. pcDNA-DEST47-SIRT1

    Plasmid

    #242131

    Purpose

    Expression in mammalian cells

    Depositor

    Izumi Horikawa, J. Carl Barrett

    Article

    Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

    Insert

    SIRT1 (SIRT1 Human)
    

    Tags

    Cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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33. pLenti-CMV-MFSD6-mNeon-Puro

    Plasmid

    #239955

    Purpose

    Lentiviral vector to generate mNeon-tagged MFSD6 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6-mNeon
    

    Use

    Lentiviral

    Tags

    mNeon

    Expression

    Mammalian

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-CMV-MFSD6-Puro

    Plasmid

    #239953

    Purpose

    Lentiviral vector to generate MFSD6 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6
    

    Use

    Lentiviral

    Tags

    myc-flag

    Expression

    Mammalian

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-CMV-MFSD8-Puro

    Plasmid

    #239954

    Purpose

    Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD8
    

    Use

    Lentiviral

    Tags

    myc-flag

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid

    #233034

    Purpose

    To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    hfCas13d and mCherry
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMR-A-3-Sasg

    Plasmid

    #214349

    Purpose

    Expresses T7-RNAP, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    T7-RNAP
    

    Expression

    Bacterial

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX6P1-NEDD4L_HECT

    Plasmid

    #187719

    Purpose

    Express GST-tagged NEDD4L HECT domain in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 594-975 (NEDD4L Human)
    

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-593

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MP6-SlugABEN-738

    Plasmid

    #214348

    Purpose

    Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
    

    Expression

    Bacterial

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBT120a17-sg-del

    Plasmid

    #214347

    Purpose

    Expresses pIII

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    gIII-xLuxAB
    

    Expression

    Bacterial

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge